Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.

Tomato (Solanum lycopersicum L.) is the second most important cultivated crop next to potato, worldwide. Tomato serves as an important source of antioxidants in human diet. Alternaria solani and Fusarium oxysporum cause early blight and vascular wilt of tomato, respectively, resulting in severe crop...

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Autores principales:	Waheed Arshad, Ihsan-ul- Haq, Mohammad Tahir Waheed, Kirankumar S Mysore, Bushra Mirza
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb7fc2e36d7b4163b84737f43080bfc5
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spelling	oai:doaj.org-article:cb7fc2e36d7b4163b84737f43080bfc52021-11-18T08:19:51ZAgrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.1932-620310.1371/journal.pone.0096979https://doaj.org/article/cb7fc2e36d7b4163b84737f43080bfc52014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24817272/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Tomato (Solanum lycopersicum L.) is the second most important cultivated crop next to potato, worldwide. Tomato serves as an important source of antioxidants in human diet. Alternaria solani and Fusarium oxysporum cause early blight and vascular wilt of tomato, respectively, resulting in severe crop losses. The foremost objective of the present study was to generate transgenic tomato plants with rolB gene and evaluate its effect on plant morphology, nutritional contents, yield and resistance against fungal infection. Tomato cv. Rio Grande was transformed via Agrobacterium tumefaciens harbouring rolB gene of Agrobacterium rhizogenes. rolB. Biochemical analyses showed considerable improvement in nutritional quality of transgenic tomato fruits as indicated by 62% increase in lycopene content, 225% in ascorbic acid content, 58% in total phenolics and 26% in free radical scavenging activity. Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum. Contrarily, transformed plants exhibited altered morphology and reduced fruit yield. In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.Waheed ArshadIhsan-ul- HaqMohammad Tahir WaheedKirankumar S MysoreBushra MirzaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96979 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Waheed Arshad Ihsan-ul- Haq Mohammad Tahir Waheed Kirankumar S Mysore Bushra Mirza Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.
description	Tomato (Solanum lycopersicum L.) is the second most important cultivated crop next to potato, worldwide. Tomato serves as an important source of antioxidants in human diet. Alternaria solani and Fusarium oxysporum cause early blight and vascular wilt of tomato, respectively, resulting in severe crop losses. The foremost objective of the present study was to generate transgenic tomato plants with rolB gene and evaluate its effect on plant morphology, nutritional contents, yield and resistance against fungal infection. Tomato cv. Rio Grande was transformed via Agrobacterium tumefaciens harbouring rolB gene of Agrobacterium rhizogenes. rolB. Biochemical analyses showed considerable improvement in nutritional quality of transgenic tomato fruits as indicated by 62% increase in lycopene content, 225% in ascorbic acid content, 58% in total phenolics and 26% in free radical scavenging activity. Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum. Contrarily, transformed plants exhibited altered morphology and reduced fruit yield. In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.
format	article
author	Waheed Arshad Ihsan-ul- Haq Mohammad Tahir Waheed Kirankumar S Mysore Bushra Mirza
author_facet	Waheed Arshad Ihsan-ul- Haq Mohammad Tahir Waheed Kirankumar S Mysore Bushra Mirza
author_sort	Waheed Arshad
title	Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.
title_short	Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.
title_full	Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.
title_fullStr	Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.
title_full_unstemmed	Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.
title_sort	agrobacterium-mediated transformation of tomato with rolb gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/cb7fc2e36d7b4163b84737f43080bfc5
work_keys_str_mv	AT waheedarshad agrobacteriummediatedtransformationoftomatowithrolbgeneresultsinenhancementoffruitqualityandfoliarresistanceagainstfungalpathogens AT ihsanulhaq agrobacteriummediatedtransformationoftomatowithrolbgeneresultsinenhancementoffruitqualityandfoliarresistanceagainstfungalpathogens AT mohammadtahirwaheed agrobacteriummediatedtransformationoftomatowithrolbgeneresultsinenhancementoffruitqualityandfoliarresistanceagainstfungalpathogens AT kirankumarsmysore agrobacteriummediatedtransformationoftomatowithrolbgeneresultsinenhancementoffruitqualityandfoliarresistanceagainstfungalpathogens AT bushramirza agrobacteriummediatedtransformationoftomatowithrolbgeneresultsinenhancementoffruitqualityandfoliarresistanceagainstfungalpathogens
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Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.

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