Next generation sequencing-aided comprehensive geographic coverage sheds light on the status of rare and extinct populations of Aporia butterflies (Lepidoptera: Pieridae)

Abstract The Black-veined White Aporia crataegi (Linnaeus, 1758), a common and widespread butterfly ranging from northwestern Africa to Europe and Asia, has been extinct in Britain since the 1920s and is on a steady decline in several other parts of its range. In order to investigate genetic diversi...

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Autores principales:	Valentina Todisco, Raluca Vodă, Sean W. J. Prosser, Vazrick Nazari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb833d059dce4dc08a2ce028e4c86224
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spelling	oai:doaj.org-article:cb833d059dce4dc08a2ce028e4c862242021-12-02T16:45:40ZNext generation sequencing-aided comprehensive geographic coverage sheds light on the status of rare and extinct populations of Aporia butterflies (Lepidoptera: Pieridae)10.1038/s41598-020-70957-42045-2322https://doaj.org/article/cb833d059dce4dc08a2ce028e4c862242020-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-70957-4https://doaj.org/toc/2045-2322Abstract The Black-veined White Aporia crataegi (Linnaeus, 1758), a common and widespread butterfly ranging from northwestern Africa to Europe and Asia, has been extinct in Britain since the 1920s and is on a steady decline in several other parts of its range. In order to investigate genetic diversity within A. crataegi and its correspondence with current subspecies-level taxonomy, we barcoded 173 specimens from across its range including, for the first time, extinct populations from Britain and Korea. Using next generation sequencing we also obtained a sequence for Aporia joubini, a peculiar taxon from China known only by its type specimen collected in the early twentieth century. Our phylogenetic analysis placed A. joubini sister to A. oberthuri, although further taxon sampling may reveal a different scheme. Within A. crataegi, we observed a shallow and weak mitogenomic structure with only a few distinct lineages in North Africa, Sicily, Iran, and Japan. Eurasian populations, including those extinct in Britain and Korea, clustered into a large set of closely allied lineages, consistent with a recent expansion during the Late Pleistocene glacial period. This study highlights the importance of museum collections and the unique opportunities they provide in documenting species diversity and helping conservation efforts.Valentina TodiscoRaluca VodăSean W. J. ProsserVazrick NazariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valentina Todisco Raluca Vodă Sean W. J. Prosser Vazrick Nazari Next generation sequencing-aided comprehensive geographic coverage sheds light on the status of rare and extinct populations of Aporia butterflies (Lepidoptera: Pieridae)
description	Abstract The Black-veined White Aporia crataegi (Linnaeus, 1758), a common and widespread butterfly ranging from northwestern Africa to Europe and Asia, has been extinct in Britain since the 1920s and is on a steady decline in several other parts of its range. In order to investigate genetic diversity within A. crataegi and its correspondence with current subspecies-level taxonomy, we barcoded 173 specimens from across its range including, for the first time, extinct populations from Britain and Korea. Using next generation sequencing we also obtained a sequence for Aporia joubini, a peculiar taxon from China known only by its type specimen collected in the early twentieth century. Our phylogenetic analysis placed A. joubini sister to A. oberthuri, although further taxon sampling may reveal a different scheme. Within A. crataegi, we observed a shallow and weak mitogenomic structure with only a few distinct lineages in North Africa, Sicily, Iran, and Japan. Eurasian populations, including those extinct in Britain and Korea, clustered into a large set of closely allied lineages, consistent with a recent expansion during the Late Pleistocene glacial period. This study highlights the importance of museum collections and the unique opportunities they provide in documenting species diversity and helping conservation efforts.
format	article
author	Valentina Todisco Raluca Vodă Sean W. J. Prosser Vazrick Nazari
author_facet	Valentina Todisco Raluca Vodă Sean W. J. Prosser Vazrick Nazari
author_sort	Valentina Todisco
title	Next generation sequencing-aided comprehensive geographic coverage sheds light on the status of rare and extinct populations of Aporia butterflies (Lepidoptera: Pieridae)
title_short	Next generation sequencing-aided comprehensive geographic coverage sheds light on the status of rare and extinct populations of Aporia butterflies (Lepidoptera: Pieridae)
title_full	Next generation sequencing-aided comprehensive geographic coverage sheds light on the status of rare and extinct populations of Aporia butterflies (Lepidoptera: Pieridae)
title_fullStr	Next generation sequencing-aided comprehensive geographic coverage sheds light on the status of rare and extinct populations of Aporia butterflies (Lepidoptera: Pieridae)
title_full_unstemmed	Next generation sequencing-aided comprehensive geographic coverage sheds light on the status of rare and extinct populations of Aporia butterflies (Lepidoptera: Pieridae)
title_sort	next generation sequencing-aided comprehensive geographic coverage sheds light on the status of rare and extinct populations of aporia butterflies (lepidoptera: pieridae)
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/cb833d059dce4dc08a2ce028e4c86224
work_keys_str_mv	AT valentinatodisco nextgenerationsequencingaidedcomprehensivegeographiccoverageshedslightonthestatusofrareandextinctpopulationsofaporiabutterflieslepidopterapieridae AT ralucavoda nextgenerationsequencingaidedcomprehensivegeographiccoverageshedslightonthestatusofrareandextinctpopulationsofaporiabutterflieslepidopterapieridae AT seanwjprosser nextgenerationsequencingaidedcomprehensivegeographiccoverageshedslightonthestatusofrareandextinctpopulationsofaporiabutterflieslepidopterapieridae AT vazricknazari nextgenerationsequencingaidedcomprehensivegeographiccoverageshedslightonthestatusofrareandextinctpopulationsofaporiabutterflieslepidopterapieridae
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Next generation sequencing-aided comprehensive geographic coverage sheds light on the status of rare and extinct populations of Aporia butterflies (Lepidoptera: Pieridae)

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