Multi-Tissue Characterization of GILZ Expression in Dendritic Cell Subsets at Steady State and in Inflammatory Contexts

Dendritic cells (DCs) are key players in the control of tolerance and immunity. Glucocorticoids (GCs) are known to regulate DC function by promoting their tolerogenic differentiation through the induction of inhibitory ligands, cytokines, and enzymes. The GC-induced effects in DCs were shown to crit...

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Autores principales:	Molène Docq, Mathias Vétillard, Carmen Gallego, Agnieszka Jaracz-Ros, Françoise Mercier-Nomé, Françoise Bachelerie, Géraldine Schlecht-Louf
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dendritic cells <i>Tsc22d3</i>/GILZ skin inflammation cancer Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cb975d3aa3e144928b435c25dff584c6
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spelling	oai:doaj.org-article:cb975d3aa3e144928b435c25dff584c62021-11-25T17:12:02ZMulti-Tissue Characterization of GILZ Expression in Dendritic Cell Subsets at Steady State and in Inflammatory Contexts10.3390/cells101131532073-4409https://doaj.org/article/cb975d3aa3e144928b435c25dff584c62021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3153https://doaj.org/toc/2073-4409Dendritic cells (DCs) are key players in the control of tolerance and immunity. Glucocorticoids (GCs) are known to regulate DC function by promoting their tolerogenic differentiation through the induction of inhibitory ligands, cytokines, and enzymes. The GC-induced effects in DCs were shown to critically depend on increased expression of the Glucocorticoid-Induced Leucine Zipper protein (GILZ). GILZ expression levels were further shown to control antigen-presenting cell function, as well as T-cell priming capacity of DCs. However, the pattern of GILZ expression in DC subsets across tissues remains poorly described, as well as the modulation of its expression levels in different pathological settings. To fill in this knowledge gap, we conducted an exhaustive analysis of GILZ relative expression levels in DC subsets from various tissues using multiparametric flow cytometry. This study was performed at steady state, in the context of acute as well as chronic skin inflammation, and in a model of cancer. Our results show the heterogeneity of GILZ expression among DC subsets as well as the complexity of its modulation, that varies in a cell subset- and context-specific manner. Considering the contribution of GILZ in the control of DC functions and its potential as an immune checkpoint in cancer settings, these results are of high relevance for optimal GILZ targeting in therapeutic strategies.Molène DocqMathias VétillardCarmen GallegoAgnieszka Jaracz-RosFrançoise Mercier-NoméFrançoise BachelerieGéraldine Schlecht-LoufMDPI AGarticledendritic cells<i>Tsc22d3</i>/GILZskininflammationcancerBiology (General)QH301-705.5ENCells, Vol 10, Iss 3153, p 3153 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dendritic cells <i>Tsc22d3</i>/GILZ skin inflammation cancer Biology (General) QH301-705.5
spellingShingle	dendritic cells <i>Tsc22d3</i>/GILZ skin inflammation cancer Biology (General) QH301-705.5 Molène Docq Mathias Vétillard Carmen Gallego Agnieszka Jaracz-Ros Françoise Mercier-Nomé Françoise Bachelerie Géraldine Schlecht-Louf Multi-Tissue Characterization of GILZ Expression in Dendritic Cell Subsets at Steady State and in Inflammatory Contexts
description	Dendritic cells (DCs) are key players in the control of tolerance and immunity. Glucocorticoids (GCs) are known to regulate DC function by promoting their tolerogenic differentiation through the induction of inhibitory ligands, cytokines, and enzymes. The GC-induced effects in DCs were shown to critically depend on increased expression of the Glucocorticoid-Induced Leucine Zipper protein (GILZ). GILZ expression levels were further shown to control antigen-presenting cell function, as well as T-cell priming capacity of DCs. However, the pattern of GILZ expression in DC subsets across tissues remains poorly described, as well as the modulation of its expression levels in different pathological settings. To fill in this knowledge gap, we conducted an exhaustive analysis of GILZ relative expression levels in DC subsets from various tissues using multiparametric flow cytometry. This study was performed at steady state, in the context of acute as well as chronic skin inflammation, and in a model of cancer. Our results show the heterogeneity of GILZ expression among DC subsets as well as the complexity of its modulation, that varies in a cell subset- and context-specific manner. Considering the contribution of GILZ in the control of DC functions and its potential as an immune checkpoint in cancer settings, these results are of high relevance for optimal GILZ targeting in therapeutic strategies.
format	article
author	Molène Docq Mathias Vétillard Carmen Gallego Agnieszka Jaracz-Ros Françoise Mercier-Nomé Françoise Bachelerie Géraldine Schlecht-Louf
author_facet	Molène Docq Mathias Vétillard Carmen Gallego Agnieszka Jaracz-Ros Françoise Mercier-Nomé Françoise Bachelerie Géraldine Schlecht-Louf
author_sort	Molène Docq
title	Multi-Tissue Characterization of GILZ Expression in Dendritic Cell Subsets at Steady State and in Inflammatory Contexts
title_short	Multi-Tissue Characterization of GILZ Expression in Dendritic Cell Subsets at Steady State and in Inflammatory Contexts
title_full	Multi-Tissue Characterization of GILZ Expression in Dendritic Cell Subsets at Steady State and in Inflammatory Contexts
title_fullStr	Multi-Tissue Characterization of GILZ Expression in Dendritic Cell Subsets at Steady State and in Inflammatory Contexts
title_full_unstemmed	Multi-Tissue Characterization of GILZ Expression in Dendritic Cell Subsets at Steady State and in Inflammatory Contexts
title_sort	multi-tissue characterization of gilz expression in dendritic cell subsets at steady state and in inflammatory contexts
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cb975d3aa3e144928b435c25dff584c6
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Multi-Tissue Characterization of GILZ Expression in Dendritic Cell Subsets at Steady State and in Inflammatory Contexts

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