The destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells

Abstract Hemorrhagic disease caused by grass carp reovirus (GCRV) has severely threatened the grass carp (Ctenopharyngodon idella) cultivation industry. It is noteworthy that the resistance against GCRV infection was reported to be inheritable, and identified at both individual and cellular levels....

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Autores principales:	Xueying Shang, Chunrong Yang, Quanyuan Wan, Youliang Rao, Jianguo Su
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb9adcc74f0d4bf2835409106a6bfffb
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spelling	oai:doaj.org-article:cb9adcc74f0d4bf2835409106a6bfffb2021-12-02T11:52:31ZThe destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells10.1038/s41598-017-03990-52045-2322https://doaj.org/article/cb9adcc74f0d4bf2835409106a6bfffb2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03990-5https://doaj.org/toc/2045-2322Abstract Hemorrhagic disease caused by grass carp reovirus (GCRV) has severely threatened the grass carp (Ctenopharyngodon idella) cultivation industry. It is noteworthy that the resistance against GCRV infection was reported to be inheritable, and identified at both individual and cellular levels. Therefore, this work was inspired and dedicated to unravel the molecular mechanisms of fate decision post GCRV infection in related immune cells. Foremost, the resistant and susceptible CIK (C. idella kidney) monoclonal cells were established by single cell sorting, subculturing and infection screening successively. RNA-Seq, MeDIP-Seq and small RNA-Seq were carried out with C1 (CIK cells), R2 (resistant cells) and S3 (susceptible cells) groups. It was demonstrated that genome-wide DNA methylation, mRNA and microRNA expression levels in S3 were the highest among three groups. Transcriptome analysis elucidated that pathways associated with antioxidant activity, cell proliferation regulation, apoptosis activity and energy consuming might contribute to the decision of cell fates post infection. And a series of immune-related genes were identified differentially expressed across resistant and susceptible groups, which were negatively modulated by DNA methylation or microRNAs. To conclude, this study systematically uncovered the regulatory mechanism on the resistance from epigenetic perspective and provided potential biomarkers for future studies on resistance breeding.Xueying ShangChunrong YangQuanyuan WanYouliang RaoJianguo SuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xueying Shang Chunrong Yang Quanyuan Wan Youliang Rao Jianguo Su The destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells
description	Abstract Hemorrhagic disease caused by grass carp reovirus (GCRV) has severely threatened the grass carp (Ctenopharyngodon idella) cultivation industry. It is noteworthy that the resistance against GCRV infection was reported to be inheritable, and identified at both individual and cellular levels. Therefore, this work was inspired and dedicated to unravel the molecular mechanisms of fate decision post GCRV infection in related immune cells. Foremost, the resistant and susceptible CIK (C. idella kidney) monoclonal cells were established by single cell sorting, subculturing and infection screening successively. RNA-Seq, MeDIP-Seq and small RNA-Seq were carried out with C1 (CIK cells), R2 (resistant cells) and S3 (susceptible cells) groups. It was demonstrated that genome-wide DNA methylation, mRNA and microRNA expression levels in S3 were the highest among three groups. Transcriptome analysis elucidated that pathways associated with antioxidant activity, cell proliferation regulation, apoptosis activity and energy consuming might contribute to the decision of cell fates post infection. And a series of immune-related genes were identified differentially expressed across resistant and susceptible groups, which were negatively modulated by DNA methylation or microRNAs. To conclude, this study systematically uncovered the regulatory mechanism on the resistance from epigenetic perspective and provided potential biomarkers for future studies on resistance breeding.
format	article
author	Xueying Shang Chunrong Yang Quanyuan Wan Youliang Rao Jianguo Su
author_facet	Xueying Shang Chunrong Yang Quanyuan Wan Youliang Rao Jianguo Su
author_sort	Xueying Shang
title	The destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells
title_short	The destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells
title_full	The destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells
title_fullStr	The destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells
title_full_unstemmed	The destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells
title_sort	destiny of the resistance/susceptibility against gcrv is controlled by epigenetic mechanisms in cik cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cb9adcc74f0d4bf2835409106a6bfffb
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The destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells

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