Effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing

Lim Jeong,1 Min Hee Kim,1 Ju-Young Jung,2 Byung Moo Min,3 Won Ho Park1 1Department of Advanced Organic Materials and Textile System Engineering, 2College of Veterinary Medicine, Chungnam National University, Daejeon, 3Department of Oral Biochemistry, School of Dentistry, Seoul National University,...

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Autores principales:	Jeong L, Kim MH, Jung JY, Min BM, Park WH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cb9f536dd8be47f39a977f9097a3e43a
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spelling	oai:doaj.org-article:cb9f536dd8be47f39a977f9097a3e43a2021-12-02T00:38:49ZEffect of silk fibroin nanofibers containing silver sulfadiazine on wound healing1178-2013https://doaj.org/article/cb9f536dd8be47f39a977f9097a3e43a2014-11-01T00:00:00Zhttp://www.dovepress.com/effect-of-silk-fibroin-nanofibers-containing-silver-sulfadiazine-on-wo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Lim Jeong,1 Min Hee Kim,1 Ju-Young Jung,2 Byung Moo Min,3 Won Ho Park1 1Department of Advanced Organic Materials and Textile System Engineering, 2College of Veterinary Medicine, Chungnam National University, Daejeon, 3Department of Oral Biochemistry, School of Dentistry, Seoul National University, Seoul, South Korea Background: One of the promising applications of silk fibroin (SF) in biomedical engineering is its use as a scaffolding material for skin regeneration. The purpose of this study was to determine the wound healing effect of SF nanofibrous matrices containing silver sulfadiazine (SSD) wound dressings.Methods: An SF nanofibrous matrix containing SSD was prepared by electrospinning. The cell attachment and spreading of normal human epidermal keratinocytes (NHEK) and normal human epidermal fibroblasts (NHEF) to SF nanofibers containing three different concentrations of SSD contents (0.1, 0.5, and 1.0 wt%) were determined. In addition, a rat wound model was used in this study to determine the wound healing effect of SF nanofibers containing SSD compared with that of Acticoat™, a commercially available wound dressing.Results: The number of NHEK and NHEF attached to SF nanofibers containing SSD decreased when the concentration of SSD increased. The number of attached NHEF cells was lower than that of attached NHEK cells. The SF matrix with 1.0 wt% SSD produced faster wound healing than Acticoat, although 1.0 wt% SSD inhibited the attachment of epidermal cells to SF nanofibers in vitro.Conclusion: The cytotoxic effects of SF nanofibers with SSD should be considered in the development of silver-release dressings for wound healing through its antimicrobial activity. It is challenging to design wound dressings that maximize antimicrobial activity and minimize cellular toxicity. Keywords: silk fibroin, silver sulfadiazine, nanofibrous matrix, wound healingJeong LKim MHJung JYMin BMPark WHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 5277-5287 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Jeong L Kim MH Jung JY Min BM Park WH Effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing
description	Lim Jeong,1 Min Hee Kim,1 Ju-Young Jung,2 Byung Moo Min,3 Won Ho Park1 1Department of Advanced Organic Materials and Textile System Engineering, 2College of Veterinary Medicine, Chungnam National University, Daejeon, 3Department of Oral Biochemistry, School of Dentistry, Seoul National University, Seoul, South Korea Background: One of the promising applications of silk fibroin (SF) in biomedical engineering is its use as a scaffolding material for skin regeneration. The purpose of this study was to determine the wound healing effect of SF nanofibrous matrices containing silver sulfadiazine (SSD) wound dressings.Methods: An SF nanofibrous matrix containing SSD was prepared by electrospinning. The cell attachment and spreading of normal human epidermal keratinocytes (NHEK) and normal human epidermal fibroblasts (NHEF) to SF nanofibers containing three different concentrations of SSD contents (0.1, 0.5, and 1.0 wt%) were determined. In addition, a rat wound model was used in this study to determine the wound healing effect of SF nanofibers containing SSD compared with that of Acticoat™, a commercially available wound dressing.Results: The number of NHEK and NHEF attached to SF nanofibers containing SSD decreased when the concentration of SSD increased. The number of attached NHEF cells was lower than that of attached NHEK cells. The SF matrix with 1.0 wt% SSD produced faster wound healing than Acticoat, although 1.0 wt% SSD inhibited the attachment of epidermal cells to SF nanofibers in vitro.Conclusion: The cytotoxic effects of SF nanofibers with SSD should be considered in the development of silver-release dressings for wound healing through its antimicrobial activity. It is challenging to design wound dressings that maximize antimicrobial activity and minimize cellular toxicity. Keywords: silk fibroin, silver sulfadiazine, nanofibrous matrix, wound healing
format	article
author	Jeong L Kim MH Jung JY Min BM Park WH
author_facet	Jeong L Kim MH Jung JY Min BM Park WH
author_sort	Jeong L
title	Effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing
title_short	Effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing
title_full	Effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing
title_fullStr	Effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing
title_full_unstemmed	Effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing
title_sort	effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/cb9f536dd8be47f39a977f9097a3e43a
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Effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing

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