Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture

Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of cul...

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Autores principales:	Ratna Dewi Eskundari, Taryono Taryono, Didik Indradewa, Yekti Asih Purwestri
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	auxin camellia sinensis cytokinin micropropagation tissue culture Agriculture (General) S1-972 Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/cba50bbd0d2848749d78c6f4b7d799cb
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spelling	oai:doaj.org-article:cba50bbd0d2848749d78c6f4b7d799cb2021-11-08T08:40:57ZMorphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture2540-95732540-958110.22146/jtbb.64403https://doaj.org/article/cba50bbd0d2848749d78c6f4b7d799cb2021-10-01T00:00:00Zhttps://jurnal.ugm.ac.id/jtbb/article/view/64403https://doaj.org/toc/2540-9573https://doaj.org/toc/2540-9581Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of culturing time has not been reported yet. This study aimed to determine the differences between those pathways, in the context of morphology, histology, and protein profile. The explants were the embryo axis of TRI2025 tea clone cultured on two different induction mediums; somatic embryogenesis and in vitro organogenesis induction medium. The results showed that most of the explants cultured on A medium developed to be a globular-like structure at 11-day after culture (DAC), while all explants cultured on B medium showed the initiation stage of in vitro organogenesis. Histological analysis showed meristem reconstruction at shoot apical meristem (SAM) and root apical meristem (RAM) at 11-DAC at explants cultured on B medium, while explants cultured on A medium showed callusing at 21-DAC. Protein profile analysis using SDS-PAGE showed protein bands of 54 and 81 KDa that only appeared at explants cultured on A medium start from 14-DAC, and those two protein bands thought to be a differentiator at the early stages of the two tissue culture techniques. Thus, these parameters can be used as early detection for plant tissue culture, especially in tea.Ratna Dewi EskundariTaryono TaryonoDidik IndradewaYekti Asih PurwestriUniversitas Gadjah Madaarticleauxincamellia sinensiscytokininmicropropagationtissue cultureAgriculture (General)S1-972Plant cultureSB1-1110ENJournal of Tropical Biodiversity and Biotechnology, Vol 6, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	auxin camellia sinensis cytokinin micropropagation tissue culture Agriculture (General) S1-972 Plant culture SB1-1110
spellingShingle	auxin camellia sinensis cytokinin micropropagation tissue culture Agriculture (General) S1-972 Plant culture SB1-1110 Ratna Dewi Eskundari Taryono Taryono Didik Indradewa Yekti Asih Purwestri Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture
description	Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of culturing time has not been reported yet. This study aimed to determine the differences between those pathways, in the context of morphology, histology, and protein profile. The explants were the embryo axis of TRI2025 tea clone cultured on two different induction mediums; somatic embryogenesis and in vitro organogenesis induction medium. The results showed that most of the explants cultured on A medium developed to be a globular-like structure at 11-day after culture (DAC), while all explants cultured on B medium showed the initiation stage of in vitro organogenesis. Histological analysis showed meristem reconstruction at shoot apical meristem (SAM) and root apical meristem (RAM) at 11-DAC at explants cultured on B medium, while explants cultured on A medium showed callusing at 21-DAC. Protein profile analysis using SDS-PAGE showed protein bands of 54 and 81 KDa that only appeared at explants cultured on A medium start from 14-DAC, and those two protein bands thought to be a differentiator at the early stages of the two tissue culture techniques. Thus, these parameters can be used as early detection for plant tissue culture, especially in tea.
format	article
author	Ratna Dewi Eskundari Taryono Taryono Didik Indradewa Yekti Asih Purwestri
author_facet	Ratna Dewi Eskundari Taryono Taryono Didik Indradewa Yekti Asih Purwestri
author_sort	Ratna Dewi Eskundari
title	Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture
title_short	Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture
title_full	Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture
title_fullStr	Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture
title_full_unstemmed	Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture
title_sort	morphological, histological, and protein profiling of tea embryo axis at early stage of culture
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/cba50bbd0d2848749d78c6f4b7d799cb
work_keys_str_mv	AT ratnadewieskundari morphologicalhistologicalandproteinprofilingofteaembryoaxisatearlystageofculture AT taryonotaryono morphologicalhistologicalandproteinprofilingofteaembryoaxisatearlystageofculture AT didikindradewa morphologicalhistologicalandproteinprofilingofteaembryoaxisatearlystageofculture AT yektiasihpurwestri morphologicalhistologicalandproteinprofilingofteaembryoaxisatearlystageofculture
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Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture

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