Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor

Abstract A philosophical shift has occurred in the field of biomedical sciences from treatment of late-stage disease symptoms to early detection and prevention. Ceria nanoparticles (CNPs) have been demonstrated to neutralize free radical chemical species associated with many life-threatening disease...

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Autores principales:	Craig J. Neal, Ankur Gupta, Swetha Barkam, Shashank Saraf, Soumen Das, Hyoung J. Cho, Sudipta Seal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cba56e62a741437bb5874cc7857a7480
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spelling	oai:doaj.org-article:cba56e62a741437bb5874cc7857a74802021-12-02T12:32:32ZPicomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor10.1038/s41598-017-01356-52045-2322https://doaj.org/article/cba56e62a741437bb5874cc7857a74802017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01356-5https://doaj.org/toc/2045-2322Abstract A philosophical shift has occurred in the field of biomedical sciences from treatment of late-stage disease symptoms to early detection and prevention. Ceria nanoparticles (CNPs) have been demonstrated to neutralize free radical chemical species associated with many life-threatening disease states such as cancers and neurodegenerative diseases by undergoing redox changes (Ce3+ ↔ Ce4+). Herein, we investigate the electrochemical response of multi-valent CNPs in presence of hydrogen peroxide and demonstrate an enzyme-free CNP-based biosensor capable of ultra-low (limit of quantitation: 0.1 pM) detection. Several preparations of CNPs with varying Ce3+:Ce4+ are produced and are analyzed by electrochemical methods. We find that an increasing magnitude of response in cyclic voltammetry and chronoamperometry correlates with increasing Ce4+ relative to Ce3+ and utilize this finding in the design of the sensor platform. The sensor retains sensitivity across a range of pH’s and temperatures, wherein enzyme-based sensors will not function, and in blood serum: reflecting selectivity and robustness as a potential implantable biomedical device.Craig J. NealAnkur GuptaSwetha BarkamShashank SarafSoumen DasHyoung J. ChoSudipta SealNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Craig J. Neal Ankur Gupta Swetha Barkam Shashank Saraf Soumen Das Hyoung J. Cho Sudipta Seal Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor
description	Abstract A philosophical shift has occurred in the field of biomedical sciences from treatment of late-stage disease symptoms to early detection and prevention. Ceria nanoparticles (CNPs) have been demonstrated to neutralize free radical chemical species associated with many life-threatening disease states such as cancers and neurodegenerative diseases by undergoing redox changes (Ce3+ ↔ Ce4+). Herein, we investigate the electrochemical response of multi-valent CNPs in presence of hydrogen peroxide and demonstrate an enzyme-free CNP-based biosensor capable of ultra-low (limit of quantitation: 0.1 pM) detection. Several preparations of CNPs with varying Ce3+:Ce4+ are produced and are analyzed by electrochemical methods. We find that an increasing magnitude of response in cyclic voltammetry and chronoamperometry correlates with increasing Ce4+ relative to Ce3+ and utilize this finding in the design of the sensor platform. The sensor retains sensitivity across a range of pH’s and temperatures, wherein enzyme-based sensors will not function, and in blood serum: reflecting selectivity and robustness as a potential implantable biomedical device.
format	article
author	Craig J. Neal Ankur Gupta Swetha Barkam Shashank Saraf Soumen Das Hyoung J. Cho Sudipta Seal
author_facet	Craig J. Neal Ankur Gupta Swetha Barkam Shashank Saraf Soumen Das Hyoung J. Cho Sudipta Seal
author_sort	Craig J. Neal
title	Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor
title_short	Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor
title_full	Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor
title_fullStr	Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor
title_full_unstemmed	Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor
title_sort	picomolar detection of hydrogen peroxide using enzyme-free inorganic nanoparticle-based sensor
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cba56e62a741437bb5874cc7857a7480
work_keys_str_mv	AT craigjneal picomolardetectionofhydrogenperoxideusingenzymefreeinorganicnanoparticlebasedsensor AT ankurgupta picomolardetectionofhydrogenperoxideusingenzymefreeinorganicnanoparticlebasedsensor AT swethabarkam picomolardetectionofhydrogenperoxideusingenzymefreeinorganicnanoparticlebasedsensor AT shashanksaraf picomolardetectionofhydrogenperoxideusingenzymefreeinorganicnanoparticlebasedsensor AT soumendas picomolardetectionofhydrogenperoxideusingenzymefreeinorganicnanoparticlebasedsensor AT hyoungjcho picomolardetectionofhydrogenperoxideusingenzymefreeinorganicnanoparticlebasedsensor AT sudiptaseal picomolardetectionofhydrogenperoxideusingenzymefreeinorganicnanoparticlebasedsensor
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Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor

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