Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR

Mustopa AZ, Puspitasari IF, Fatimah, Triratna L, Kartina G. 2018. Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR. Biodiversitas 19: 1714-1721. Mastitis in cow is caused by several pathogenic bacteria, including antibiotic-resistant bacteria. Identification of the pathogenic bact...

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Autores principales:	APON ZAENAL MUSTOPA, INES FERDIANA PUSPITASARI, FATIMAH FATIMAH, LITA TRIRATNA, GINA KARTINA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	16s rrna analysis mastitis cow’s milk bacteria rapd-pcr analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cba9446d4b5a4a5fa99dcbeceb4b4e7f
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spelling	oai:doaj.org-article:cba9446d4b5a4a5fa99dcbeceb4b4e7f2021-11-16T14:04:05ZGenetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR1412-033X2085-472210.13057/biodiv/d190517https://doaj.org/article/cba9446d4b5a4a5fa99dcbeceb4b4e7f2018-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2934https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Mustopa AZ, Puspitasari IF, Fatimah, Triratna L, Kartina G. 2018. Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR. Biodiversitas 19: 1714-1721. Mastitis in cow is caused by several pathogenic bacteria, including antibiotic-resistant bacteria. Identification of the pathogenic bacteria’s diversity that is contaminating cow’s milk needs to be done. The aim of this research was to conduct molecular identification in mastitis cow’s milk bacteria through RAPD-PCR (Random Amplified Polymorphism DNA-Polymerase Chain Reaction) analysis. Bacteria from mastitis cow’s milk were enumerated using selective media. Based on the result of media selection, there were 72 isolates of bacteria from mastitis cows in Ciguha, Guranteng, Cikarenceng Village (Pagerageung, Tasikmalaya), and Warnasari Village (Pangalengan, Bandung), West Java, Indonesia. The genomes from these isolates were extracted and then subjected to RAPD-PCR analysis. The results of RAPD-PCR analysis showed 8 clusters of dendrogram which 4 dominant clusters were selected. Identification of 4 dominant clusters, which contained representative strains, using 16s rRNA showed the isolates BPA-12, MHA-6, L-4, and XLDA-8 were identified as Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, and Enterobacter bugandensis.APON ZAENAL MUSTOPAINES FERDIANA PUSPITASARIFATIMAH FATIMAHLITA TRIRATNAGINA KARTINAMBI & UNS Soloarticle16s rrna analysismastitis cow’s milk bacteriarapd-pcr analysisBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 5, Pp 1714-1721 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna analysis mastitis cow’s milk bacteria rapd-pcr analysis Biology (General) QH301-705.5
spellingShingle	16s rrna analysis mastitis cow’s milk bacteria rapd-pcr analysis Biology (General) QH301-705.5 APON ZAENAL MUSTOPA INES FERDIANA PUSPITASARI FATIMAH FATIMAH LITA TRIRATNA GINA KARTINA Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR
description	Mustopa AZ, Puspitasari IF, Fatimah, Triratna L, Kartina G. 2018. Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR. Biodiversitas 19: 1714-1721. Mastitis in cow is caused by several pathogenic bacteria, including antibiotic-resistant bacteria. Identification of the pathogenic bacteria’s diversity that is contaminating cow’s milk needs to be done. The aim of this research was to conduct molecular identification in mastitis cow’s milk bacteria through RAPD-PCR (Random Amplified Polymorphism DNA-Polymerase Chain Reaction) analysis. Bacteria from mastitis cow’s milk were enumerated using selective media. Based on the result of media selection, there were 72 isolates of bacteria from mastitis cows in Ciguha, Guranteng, Cikarenceng Village (Pagerageung, Tasikmalaya), and Warnasari Village (Pangalengan, Bandung), West Java, Indonesia. The genomes from these isolates were extracted and then subjected to RAPD-PCR analysis. The results of RAPD-PCR analysis showed 8 clusters of dendrogram which 4 dominant clusters were selected. Identification of 4 dominant clusters, which contained representative strains, using 16s rRNA showed the isolates BPA-12, MHA-6, L-4, and XLDA-8 were identified as Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, and Enterobacter bugandensis.
format	article
author	APON ZAENAL MUSTOPA INES FERDIANA PUSPITASARI FATIMAH FATIMAH LITA TRIRATNA GINA KARTINA
author_facet	APON ZAENAL MUSTOPA INES FERDIANA PUSPITASARI FATIMAH FATIMAH LITA TRIRATNA GINA KARTINA
author_sort	APON ZAENAL MUSTOPA
title	Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR
title_short	Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR
title_full	Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR
title_fullStr	Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR
title_full_unstemmed	Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR
title_sort	genetic diversity of mastitis cow’s milk bacteria based on rapd-pcr
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/cba9446d4b5a4a5fa99dcbeceb4b4e7f
work_keys_str_mv	AT aponzaenalmustopa geneticdiversityofmastitiscowsmilkbacteriabasedonrapdpcr AT inesferdianapuspitasari geneticdiversityofmastitiscowsmilkbacteriabasedonrapdpcr AT fatimahfatimah geneticdiversityofmastitiscowsmilkbacteriabasedonrapdpcr AT litatriratna geneticdiversityofmastitiscowsmilkbacteriabasedonrapdpcr AT ginakartina geneticdiversityofmastitiscowsmilkbacteriabasedonrapdpcr
_version_	1718426449173544960

Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR

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