Post-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (Felis catus).

The effects of pre- (i.e., gestation and during lactation) and post-weaning diet on the composition of faecal bacterial communities and adipose expression of key genes in the glucose and insulin pathways were investigated in the cat. Queens were maintained on a moderate protein:fat:carbohydrate kibb...

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Autores principales:	Emma N Bermingham, Sandra Kittelmann, Wayne Young, Katherine R Kerr, Kelly S Swanson, Nicole C Roy, David G Thomas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cbaaf34255834694b785482e40161cda
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spelling	oai:doaj.org-article:cbaaf34255834694b785482e40161cda2021-11-18T08:44:18ZPost-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (Felis catus).1932-620310.1371/journal.pone.0080992https://doaj.org/article/cbaaf34255834694b785482e40161cda2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24312255/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The effects of pre- (i.e., gestation and during lactation) and post-weaning diet on the composition of faecal bacterial communities and adipose expression of key genes in the glucose and insulin pathways were investigated in the cat. Queens were maintained on a moderate protein:fat:carbohydrate kibbled ("Diet A"; 35:20:28% DM; n = 4) or high protein:fat:carbohydrate canned ("Diet B"; 45:37:2% DM; n = 3) diet throughout pregnancy and lactation. Offspring were weaned onto these diets in a nested design (n = 5 per treatment). Faecal samples were collected at wk 8 and 17 of age. DNA was isolated from faeces and bacterial 16S rRNA gene amplicons were analysed by pyrosequencing. RNA was extracted from blood (wk 18) and adipose tissue and ovarian/testicular tissues (wk 24) and gene expression levels determined using RT-qPCR. Differences (P<0.05) in composition of faecal bacteria were observed between pregnant queens fed Diet A or B. However, pre-weaning diet had little effect on faecal bacterial composition in weaned kittens. In contrast, post-weaning diet altered bacterial population profiles in the kittens. Increased (P<0.05) abundance of Firmicutes (77% vs 52% of total reads) and Actinobacteria (0.8% vs 0.2% of total reads), and decreased (P<0.05) abundance of Fusobacteria (1.6% vs 18.4% of total reads) were observed for kittens fed the Diet A compared to those fed Diet B post-weaning. Feeding Diet B pre-weaning increased (P<0.05) the expression levels of INRS, LEPT, PAI-1 and tended to increase GLUT1, while the expression levels of IRS-1 in blood increased in kittens fed Diet A pre-weaning. Post-weaning diet had no effect on expression levels of target genes. Correlations between the expression levels of genes involved in glucose and insulin pathways and faecal Bacteriodetes and Firmicutes phyla were identified. The reasons for why post-weaning diet affects microbial populations and not gene expression levels are of interest.Emma N BerminghamSandra KittelmannWayne YoungKatherine R KerrKelly S SwansonNicole C RoyDavid G ThomasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80992 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emma N Bermingham Sandra Kittelmann Wayne Young Katherine R Kerr Kelly S Swanson Nicole C Roy David G Thomas Post-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (Felis catus).
description	The effects of pre- (i.e., gestation and during lactation) and post-weaning diet on the composition of faecal bacterial communities and adipose expression of key genes in the glucose and insulin pathways were investigated in the cat. Queens were maintained on a moderate protein:fat:carbohydrate kibbled ("Diet A"; 35:20:28% DM; n = 4) or high protein:fat:carbohydrate canned ("Diet B"; 45:37:2% DM; n = 3) diet throughout pregnancy and lactation. Offspring were weaned onto these diets in a nested design (n = 5 per treatment). Faecal samples were collected at wk 8 and 17 of age. DNA was isolated from faeces and bacterial 16S rRNA gene amplicons were analysed by pyrosequencing. RNA was extracted from blood (wk 18) and adipose tissue and ovarian/testicular tissues (wk 24) and gene expression levels determined using RT-qPCR. Differences (P<0.05) in composition of faecal bacteria were observed between pregnant queens fed Diet A or B. However, pre-weaning diet had little effect on faecal bacterial composition in weaned kittens. In contrast, post-weaning diet altered bacterial population profiles in the kittens. Increased (P<0.05) abundance of Firmicutes (77% vs 52% of total reads) and Actinobacteria (0.8% vs 0.2% of total reads), and decreased (P<0.05) abundance of Fusobacteria (1.6% vs 18.4% of total reads) were observed for kittens fed the Diet A compared to those fed Diet B post-weaning. Feeding Diet B pre-weaning increased (P<0.05) the expression levels of INRS, LEPT, PAI-1 and tended to increase GLUT1, while the expression levels of IRS-1 in blood increased in kittens fed Diet A pre-weaning. Post-weaning diet had no effect on expression levels of target genes. Correlations between the expression levels of genes involved in glucose and insulin pathways and faecal Bacteriodetes and Firmicutes phyla were identified. The reasons for why post-weaning diet affects microbial populations and not gene expression levels are of interest.
format	article
author	Emma N Bermingham Sandra Kittelmann Wayne Young Katherine R Kerr Kelly S Swanson Nicole C Roy David G Thomas
author_facet	Emma N Bermingham Sandra Kittelmann Wayne Young Katherine R Kerr Kelly S Swanson Nicole C Roy David G Thomas
author_sort	Emma N Bermingham
title	Post-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (Felis catus).
title_short	Post-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (Felis catus).
title_full	Post-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (Felis catus).
title_fullStr	Post-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (Felis catus).
title_full_unstemmed	Post-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (Felis catus).
title_sort	post-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (felis catus).
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cbaaf34255834694b785482e40161cda
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Post-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (Felis catus).

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