Comprehensive Transcriptome Profiles of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159 Map Core Streptococcal Competence Genes

ABSTRACT In Streptococcus mutans, an oral colonizer associated with dental caries, development of competence for natural genetic transformation is triggered by either of two types of peptide pheromones, competence-stimulating peptides (CSPs) (18 amino acids [aa]) or SigX-inducing peptides (XIPs) (7...

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Autores principales:	R. Khan, H. V. Rukke, H. Høvik, H. A. Åmdal, T. Chen, D. A. Morrison, F. C. Petersen
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	CSP Streptococcus XIP genetic competence natural transformation systems pheromone Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/cbad80e3dc6546c1b6ff7206a82d1870
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spelling	oai:doaj.org-article:cbad80e3dc6546c1b6ff7206a82d18702021-12-02T19:48:49ZComprehensive Transcriptome Profiles of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159 Map Core Streptococcal Competence Genes10.1128/mSystems.00038-152379-5077https://doaj.org/article/cbad80e3dc6546c1b6ff7206a82d18702016-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00038-15https://doaj.org/toc/2379-5077ABSTRACT In Streptococcus mutans, an oral colonizer associated with dental caries, development of competence for natural genetic transformation is triggered by either of two types of peptide pheromones, competence-stimulating peptides (CSPs) (18 amino acids [aa]) or SigX-inducing peptides (XIPs) (7 aa). Competence induced by CSP is a late response to the pheromone that requires the response regulator ComE and the XIP-encoding gene comS. XIP binds to ComR to allow expression of the alternative sigma factor SigX and the effector genes it controls. While these regulatory links are established, the precise set of effectors controlled by each regulator is poorly defined. To improve the definition of all three regulons, we used a high-resolution tiling array to map global changes in gene expression in the early and late phases of the CSP response. The early phase of the CSP response was limited to increased gene expression at four loci associated with bacteriocin production and immunity. In the late phase, upregulated regions expanded to a total of 29 loci, including comS and genes required for DNA uptake and recombination. The results indicate that the entire late response to CSP depends on the expression of comS and that the immediate transcriptional response to CSP, mediated by ComE, is restricted to just four bacteriocin-related loci. Comparison of the new data with published transcriptome data permitted the identification of all of the operons in each regulon: 4 for ComE, 2 for ComR, and 21 for SigX. Finally, a core set of 27 panstreptococcal competence genes was identified within the SigX regulon by comparison of transcriptome data from diverse streptococcal species. IMPORTANCE S. mutans has the hard surfaces of the oral cavity as its natural habitat, where it depends on its ability to form biofilms in order to survive. The comprehensive identification of S. mutans regulons activated in response to peptide pheromones provides an important basis for understanding how S. mutans can transition from individual to social behavior. Our study placed 27 of the 29 transcripts activated during competence within three major regulons and revealed a core set of 27 panstreptococcal competence-activated genes within the SigX regulon.R. KhanH. V. RukkeH. HøvikH. A. ÅmdalT. ChenD. A. MorrisonF. C. PetersenAmerican Society for MicrobiologyarticleCSPStreptococcusXIPgenetic competencenatural transformation systemspheromoneMicrobiologyQR1-502ENmSystems, Vol 1, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	CSP Streptococcus XIP genetic competence natural transformation systems pheromone Microbiology QR1-502
spellingShingle	CSP Streptococcus XIP genetic competence natural transformation systems pheromone Microbiology QR1-502 R. Khan H. V. Rukke H. Høvik H. A. Åmdal T. Chen D. A. Morrison F. C. Petersen Comprehensive Transcriptome Profiles of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159 Map Core Streptococcal Competence Genes
description	ABSTRACT In Streptococcus mutans, an oral colonizer associated with dental caries, development of competence for natural genetic transformation is triggered by either of two types of peptide pheromones, competence-stimulating peptides (CSPs) (18 amino acids [aa]) or SigX-inducing peptides (XIPs) (7 aa). Competence induced by CSP is a late response to the pheromone that requires the response regulator ComE and the XIP-encoding gene comS. XIP binds to ComR to allow expression of the alternative sigma factor SigX and the effector genes it controls. While these regulatory links are established, the precise set of effectors controlled by each regulator is poorly defined. To improve the definition of all three regulons, we used a high-resolution tiling array to map global changes in gene expression in the early and late phases of the CSP response. The early phase of the CSP response was limited to increased gene expression at four loci associated with bacteriocin production and immunity. In the late phase, upregulated regions expanded to a total of 29 loci, including comS and genes required for DNA uptake and recombination. The results indicate that the entire late response to CSP depends on the expression of comS and that the immediate transcriptional response to CSP, mediated by ComE, is restricted to just four bacteriocin-related loci. Comparison of the new data with published transcriptome data permitted the identification of all of the operons in each regulon: 4 for ComE, 2 for ComR, and 21 for SigX. Finally, a core set of 27 panstreptococcal competence genes was identified within the SigX regulon by comparison of transcriptome data from diverse streptococcal species. IMPORTANCE S. mutans has the hard surfaces of the oral cavity as its natural habitat, where it depends on its ability to form biofilms in order to survive. The comprehensive identification of S. mutans regulons activated in response to peptide pheromones provides an important basis for understanding how S. mutans can transition from individual to social behavior. Our study placed 27 of the 29 transcripts activated during competence within three major regulons and revealed a core set of 27 panstreptococcal competence-activated genes within the SigX regulon.
format	article
author	R. Khan H. V. Rukke H. Høvik H. A. Åmdal T. Chen D. A. Morrison F. C. Petersen
author_facet	R. Khan H. V. Rukke H. Høvik H. A. Åmdal T. Chen D. A. Morrison F. C. Petersen
author_sort	R. Khan
title	Comprehensive Transcriptome Profiles of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159 Map Core Streptococcal Competence Genes
title_short	Comprehensive Transcriptome Profiles of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159 Map Core Streptococcal Competence Genes
title_full	Comprehensive Transcriptome Profiles of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159 Map Core Streptococcal Competence Genes
title_fullStr	Comprehensive Transcriptome Profiles of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159 Map Core Streptococcal Competence Genes
title_full_unstemmed	Comprehensive Transcriptome Profiles of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159 Map Core Streptococcal Competence Genes
title_sort	comprehensive transcriptome profiles of <named-content content-type="genus-species">streptococcus mutans</named-content> ua159 map core streptococcal competence genes
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/cbad80e3dc6546c1b6ff7206a82d1870
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Comprehensive Transcriptome Profiles of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159 Map Core Streptococcal Competence Genes

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