Discrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy

Fourier transform infrared (FTIR) micro-spectroscopy has been attracting the interest of many cytologists and histopathologists for several years. This is related to the possibility of FTIR translation in the clinical diagnostic field. In fact, FTIR spectra are able to detect changes in biochemical...

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Autores principales:	Maria Lasalvia, Vito Capozzi, Giuseppe Perna
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	FTIR micro-spectroscopy breast cell lines principal component analysis Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/cbb02a25b3984f35b7a6dc1db9c56b79
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spelling	oai:doaj.org-article:cbb02a25b3984f35b7a6dc1db9c56b792021-11-11T19:02:04ZDiscrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy10.3390/s212169921424-8220https://doaj.org/article/cbb02a25b3984f35b7a6dc1db9c56b792021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/21/6992https://doaj.org/toc/1424-8220Fourier transform infrared (FTIR) micro-spectroscopy has been attracting the interest of many cytologists and histopathologists for several years. This is related to the possibility of FTIR translation in the clinical diagnostic field. In fact, FTIR spectra are able to detect changes in biochemical cellular components occurring when the cells pass to a pathological state. Recently, this interest has increased because it has been shown that FTIR spectra carried out just in the high wavenumber spectral range (2500–4000 cm<sup>−1</sup>), where information mainly relating to lipids and proteins can be obtained, are able to discriminate cell lines related to different tissues. This possibility allows to perform IR absorption measurements of cellular samples deposited onto microscopy glass slides (widely used in the medical environment) which are transparent to IR radiation only for wavenumber values larger than 2000 cm<sup>−1</sup>. For these reasons, we show that FTIR spectra in the 2800–3000 cm<sup>−1</sup> spectral range can discriminate three different cell lines from breast tissue: a non-malignant cell line (MCF10A), a non-metastatic adenocarcinoma cell line (MCF7) and a metastatic adenocarcinoma cell line (MDA). All the cells were grown onto glass slides. The spectra were discriminated by means of a principal component analysis, according to the PC1 component, whose values have the opposite sign in the pairwise score plots. This result supports the wide studies that are being carried out to promote the translation of the FTIR technique in medical practice, as a complementary diagnostic tool.Maria LasalviaVito CapozziGiuseppe PernaMDPI AGarticleFTIR micro-spectroscopybreast cell linesprincipal component analysisChemical technologyTP1-1185ENSensors, Vol 21, Iss 6992, p 6992 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	FTIR micro-spectroscopy breast cell lines principal component analysis Chemical technology TP1-1185
spellingShingle	FTIR micro-spectroscopy breast cell lines principal component analysis Chemical technology TP1-1185 Maria Lasalvia Vito Capozzi Giuseppe Perna Discrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy
description	Fourier transform infrared (FTIR) micro-spectroscopy has been attracting the interest of many cytologists and histopathologists for several years. This is related to the possibility of FTIR translation in the clinical diagnostic field. In fact, FTIR spectra are able to detect changes in biochemical cellular components occurring when the cells pass to a pathological state. Recently, this interest has increased because it has been shown that FTIR spectra carried out just in the high wavenumber spectral range (2500–4000 cm<sup>−1</sup>), where information mainly relating to lipids and proteins can be obtained, are able to discriminate cell lines related to different tissues. This possibility allows to perform IR absorption measurements of cellular samples deposited onto microscopy glass slides (widely used in the medical environment) which are transparent to IR radiation only for wavenumber values larger than 2000 cm<sup>−1</sup>. For these reasons, we show that FTIR spectra in the 2800–3000 cm<sup>−1</sup> spectral range can discriminate three different cell lines from breast tissue: a non-malignant cell line (MCF10A), a non-metastatic adenocarcinoma cell line (MCF7) and a metastatic adenocarcinoma cell line (MDA). All the cells were grown onto glass slides. The spectra were discriminated by means of a principal component analysis, according to the PC1 component, whose values have the opposite sign in the pairwise score plots. This result supports the wide studies that are being carried out to promote the translation of the FTIR technique in medical practice, as a complementary diagnostic tool.
format	article
author	Maria Lasalvia Vito Capozzi Giuseppe Perna
author_facet	Maria Lasalvia Vito Capozzi Giuseppe Perna
author_sort	Maria Lasalvia
title	Discrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy
title_short	Discrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy
title_full	Discrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy
title_fullStr	Discrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy
title_full_unstemmed	Discrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy
title_sort	discrimination of different breast cell lines on glass substrate by means of fourier transform infrared spectroscopy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cbb02a25b3984f35b7a6dc1db9c56b79
work_keys_str_mv	AT marialasalvia discriminationofdifferentbreastcelllinesonglasssubstratebymeansoffouriertransforminfraredspectroscopy AT vitocapozzi discriminationofdifferentbreastcelllinesonglasssubstratebymeansoffouriertransforminfraredspectroscopy AT giuseppeperna discriminationofdifferentbreastcelllinesonglasssubstratebymeansoffouriertransforminfraredspectroscopy
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Discrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy

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