Characterization of Virus Replication, Pathogenesis, and Cytokine Responses in Syrian Hamsters Inoculated with SARS-CoV-2

Shiu-Ju Yang,1 Ting-Chun Wei,1 Chih-Hao Hsu,1 Sin-Ni Ho,1 Chi-Yun Lai,2 Shiu-Feng Huang,2,3 Yih-Yuan Chen,4 Shih-Jen Liu,1 Guann-Yi Yu,1 Horng-Yunn Dou1,5 1National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Miaoli, 35053, Taiwan; 2Pathology Core L...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yang SJ, Wei TC, Hsu CH, Ho SN, Lai CY, Huang SF, Chen YY, Liu SJ, Yu GY, Dou HY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	sars-cov-2 hamster immune response pathogenesis Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/cbb47704206a4ca5b9beb108c462f414
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cbb47704206a4ca5b9beb108c462f414
record_format	dspace
spelling	oai:doaj.org-article:cbb47704206a4ca5b9beb108c462f4142021-12-02T18:50:07ZCharacterization of Virus Replication, Pathogenesis, and Cytokine Responses in Syrian Hamsters Inoculated with SARS-CoV-21178-7031https://doaj.org/article/cbb47704206a4ca5b9beb108c462f4142021-08-01T00:00:00Zhttps://www.dovepress.com/characterization-of-virus-replication-pathogenesis-and-cytokine-respon-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Shiu-Ju Yang,1 Ting-Chun Wei,1 Chih-Hao Hsu,1 Sin-Ni Ho,1 Chi-Yun Lai,2 Shiu-Feng Huang,2,3 Yih-Yuan Chen,4 Shih-Jen Liu,1 Guann-Yi Yu,1 Horng-Yunn Dou1,5 1National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Miaoli, 35053, Taiwan; 2Pathology Core Laboratory, National Health Research Institutes, Zhunan, Miaoli, 35053, Taiwan; 3National Institute of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan, Miaoli, 35053, Taiwan; 4Department of Biochemical Science and Technology, National Chiayi University, Chia-Yi, 60070, Taiwan; 5Department of Biological Science & Technology, National Yang Ming Chiao Tung University, Hsinchu, TaiwanCorrespondence: Horng-Yunn DouNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, 35, Keyan Road, Zhunan Town, Miaoli County, 35053, TaiwanTel +886-37-206-166 ext 35529Fax +886-37-583-009Email hydou@nhri.edu.twBackground: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus which caused a global respiratory disease pandemic beginning in December 2019. Understanding the pathogenesis of infection and the immune responses in a SARS-CoV-2-infected animal model is urgently needed for vaccine development.Methods: Syrian hamsters (Mesocricetus auratus) were intranasally inoculated with 105, 5× 105, and 106 TCID50 of SARS-CoV-2 per animal and studied for up to 14 days. Body weight, viral load and real-time PCR amplification of the SARS-CoV-2 N gene were measured. On days 3, 6 and 9, lung, blood, liver, pancreas, heart, kidney, and bone marrow were harvested and processed for pathology, viral load, and cytokine expression.Results: Body weight loss, increased viral load, immune cell infiltration, upregulated cytokine expression, viral RNA, SARS-CoV-2 nucleoprotein, and mucus were detected in the lungs, particularly on day 3 post-infection. Extremely high expression of the pro-inflammatory cytokines MIP-1 and RANTES was detected in lung tissue, as was high expression of IL-1β, IL-6, IL-12, and PD-L1. The glutamic oxalacetic transaminase/glutamic pyruvic transaminase (GOT/GPT) ratio in blood was significantly increased at 6 days post-infection, and plasma amylase and lipase levels were also elevated in infected hamsters.Conclusion: Our results provide new information on immunological cytokines and biological parameters related to the pathogenesis and immune response profile in the Syrian hamster model of SARS-CoV-2 infection.Keywords: SARS-CoV-2, hamster, immune response, pathogenesisYang SJWei TCHsu CHHo SNLai CYHuang SFChen YYLiu SJYu GYDou HYDove Medical Pressarticlesars-cov-2hamsterimmune responsepathogenesisPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 3781-3795 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sars-cov-2 hamster immune response pathogenesis Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	sars-cov-2 hamster immune response pathogenesis Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Yang SJ Wei TC Hsu CH Ho SN Lai CY Huang SF Chen YY Liu SJ Yu GY Dou HY Characterization of Virus Replication, Pathogenesis, and Cytokine Responses in Syrian Hamsters Inoculated with SARS-CoV-2
description	Shiu-Ju Yang,1 Ting-Chun Wei,1 Chih-Hao Hsu,1 Sin-Ni Ho,1 Chi-Yun Lai,2 Shiu-Feng Huang,2,3 Yih-Yuan Chen,4 Shih-Jen Liu,1 Guann-Yi Yu,1 Horng-Yunn Dou1,5 1National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Miaoli, 35053, Taiwan; 2Pathology Core Laboratory, National Health Research Institutes, Zhunan, Miaoli, 35053, Taiwan; 3National Institute of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan, Miaoli, 35053, Taiwan; 4Department of Biochemical Science and Technology, National Chiayi University, Chia-Yi, 60070, Taiwan; 5Department of Biological Science & Technology, National Yang Ming Chiao Tung University, Hsinchu, TaiwanCorrespondence: Horng-Yunn DouNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, 35, Keyan Road, Zhunan Town, Miaoli County, 35053, TaiwanTel +886-37-206-166 ext 35529Fax +886-37-583-009Email hydou@nhri.edu.twBackground: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus which caused a global respiratory disease pandemic beginning in December 2019. Understanding the pathogenesis of infection and the immune responses in a SARS-CoV-2-infected animal model is urgently needed for vaccine development.Methods: Syrian hamsters (Mesocricetus auratus) were intranasally inoculated with 105, 5× 105, and 106 TCID50 of SARS-CoV-2 per animal and studied for up to 14 days. Body weight, viral load and real-time PCR amplification of the SARS-CoV-2 N gene were measured. On days 3, 6 and 9, lung, blood, liver, pancreas, heart, kidney, and bone marrow were harvested and processed for pathology, viral load, and cytokine expression.Results: Body weight loss, increased viral load, immune cell infiltration, upregulated cytokine expression, viral RNA, SARS-CoV-2 nucleoprotein, and mucus were detected in the lungs, particularly on day 3 post-infection. Extremely high expression of the pro-inflammatory cytokines MIP-1 and RANTES was detected in lung tissue, as was high expression of IL-1β, IL-6, IL-12, and PD-L1. The glutamic oxalacetic transaminase/glutamic pyruvic transaminase (GOT/GPT) ratio in blood was significantly increased at 6 days post-infection, and plasma amylase and lipase levels were also elevated in infected hamsters.Conclusion: Our results provide new information on immunological cytokines and biological parameters related to the pathogenesis and immune response profile in the Syrian hamster model of SARS-CoV-2 infection.Keywords: SARS-CoV-2, hamster, immune response, pathogenesis
format	article
author	Yang SJ Wei TC Hsu CH Ho SN Lai CY Huang SF Chen YY Liu SJ Yu GY Dou HY
author_facet	Yang SJ Wei TC Hsu CH Ho SN Lai CY Huang SF Chen YY Liu SJ Yu GY Dou HY
author_sort	Yang SJ
title	Characterization of Virus Replication, Pathogenesis, and Cytokine Responses in Syrian Hamsters Inoculated with SARS-CoV-2
title_short	Characterization of Virus Replication, Pathogenesis, and Cytokine Responses in Syrian Hamsters Inoculated with SARS-CoV-2
title_full	Characterization of Virus Replication, Pathogenesis, and Cytokine Responses in Syrian Hamsters Inoculated with SARS-CoV-2
title_fullStr	Characterization of Virus Replication, Pathogenesis, and Cytokine Responses in Syrian Hamsters Inoculated with SARS-CoV-2
title_full_unstemmed	Characterization of Virus Replication, Pathogenesis, and Cytokine Responses in Syrian Hamsters Inoculated with SARS-CoV-2
title_sort	characterization of virus replication, pathogenesis, and cytokine responses in syrian hamsters inoculated with sars-cov-2
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/cbb47704206a4ca5b9beb108c462f414
work_keys_str_mv	AT yangsj characterizationofvirusreplicationpathogenesisandcytokineresponsesinsyrianhamstersinoculatedwithsarscov2 AT weitc characterizationofvirusreplicationpathogenesisandcytokineresponsesinsyrianhamstersinoculatedwithsarscov2 AT hsuch characterizationofvirusreplicationpathogenesisandcytokineresponsesinsyrianhamstersinoculatedwithsarscov2 AT hosn characterizationofvirusreplicationpathogenesisandcytokineresponsesinsyrianhamstersinoculatedwithsarscov2 AT laicy characterizationofvirusreplicationpathogenesisandcytokineresponsesinsyrianhamstersinoculatedwithsarscov2 AT huangsf characterizationofvirusreplicationpathogenesisandcytokineresponsesinsyrianhamstersinoculatedwithsarscov2 AT chenyy characterizationofvirusreplicationpathogenesisandcytokineresponsesinsyrianhamstersinoculatedwithsarscov2 AT liusj characterizationofvirusreplicationpathogenesisandcytokineresponsesinsyrianhamstersinoculatedwithsarscov2 AT yugy characterizationofvirusreplicationpathogenesisandcytokineresponsesinsyrianhamstersinoculatedwithsarscov2 AT douhy characterizationofvirusreplicationpathogenesisandcytokineresponsesinsyrianhamstersinoculatedwithsarscov2
_version_	1718377532520136704

Characterization of Virus Replication, Pathogenesis, and Cytokine Responses in Syrian Hamsters Inoculated with SARS-CoV-2

Ejemplares similares