Early life stress induces age-dependent epigenetic changes in p11 gene expression in male mice

Abstract Early life stress (ELS) causes long-lasting changes in gene expression through epigenetic mechanisms. However, little is known about the effects of ELS in adulthood, specifically across different age groups. In this study, the epigenetic modifications of p11 expression in adult mice subject...

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Autores principales:	Mi Kyoung Seo, Jung Goo Lee, Sung Woo Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cbb4ceffde4a4676832084b69846e2b8
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spelling	oai:doaj.org-article:cbb4ceffde4a4676832084b69846e2b82021-12-02T15:28:57ZEarly life stress induces age-dependent epigenetic changes in p11 gene expression in male mice10.1038/s41598-021-89593-72045-2322https://doaj.org/article/cbb4ceffde4a4676832084b69846e2b82021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89593-7https://doaj.org/toc/2045-2322Abstract Early life stress (ELS) causes long-lasting changes in gene expression through epigenetic mechanisms. However, little is known about the effects of ELS in adulthood, specifically across different age groups. In this study, the epigenetic modifications of p11 expression in adult mice subjected to ELS were investigated in different stages of adulthood. Pups experienced maternal separation (MS) for 3 h daily from postnatal day 1 to 21. At young and middle adulthood, behavioral test, hippocampal p11 expression levels, and levels of histone acetylation and methylation and DNA methylation at the hippocampal p11 promoter were measured. Middle-aged, but not young adult, MS mice exhibited increased immobility time in the forced swimming test. Concurrent with reduced hippocampal p11 levels, mice in both age groups showed a decrease in histone acetylation (AcH3) and permissive histone methylation (H3K4me3) at the p11 promoter, as well as an increase in repressive histone methylation (H3K27me3). Moreover, our results showed that the expression, AcH3 and H3Kme3 levels of p11 gene in response to MS were reduced with age. DNA methylation analysis of the p11 promoter revealed increased CpG methylation in middle-aged MS mice only. The results highlight the age-dependent deleterious effects of ELS on the epigenetic modifications of p11 transcription.Mi Kyoung SeoJung Goo LeeSung Woo ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mi Kyoung Seo Jung Goo Lee Sung Woo Park Early life stress induces age-dependent epigenetic changes in p11 gene expression in male mice
description	Abstract Early life stress (ELS) causes long-lasting changes in gene expression through epigenetic mechanisms. However, little is known about the effects of ELS in adulthood, specifically across different age groups. In this study, the epigenetic modifications of p11 expression in adult mice subjected to ELS were investigated in different stages of adulthood. Pups experienced maternal separation (MS) for 3 h daily from postnatal day 1 to 21. At young and middle adulthood, behavioral test, hippocampal p11 expression levels, and levels of histone acetylation and methylation and DNA methylation at the hippocampal p11 promoter were measured. Middle-aged, but not young adult, MS mice exhibited increased immobility time in the forced swimming test. Concurrent with reduced hippocampal p11 levels, mice in both age groups showed a decrease in histone acetylation (AcH3) and permissive histone methylation (H3K4me3) at the p11 promoter, as well as an increase in repressive histone methylation (H3K27me3). Moreover, our results showed that the expression, AcH3 and H3Kme3 levels of p11 gene in response to MS were reduced with age. DNA methylation analysis of the p11 promoter revealed increased CpG methylation in middle-aged MS mice only. The results highlight the age-dependent deleterious effects of ELS on the epigenetic modifications of p11 transcription.
format	article
author	Mi Kyoung Seo Jung Goo Lee Sung Woo Park
author_facet	Mi Kyoung Seo Jung Goo Lee Sung Woo Park
author_sort	Mi Kyoung Seo
title	Early life stress induces age-dependent epigenetic changes in p11 gene expression in male mice
title_short	Early life stress induces age-dependent epigenetic changes in p11 gene expression in male mice
title_full	Early life stress induces age-dependent epigenetic changes in p11 gene expression in male mice
title_fullStr	Early life stress induces age-dependent epigenetic changes in p11 gene expression in male mice
title_full_unstemmed	Early life stress induces age-dependent epigenetic changes in p11 gene expression in male mice
title_sort	early life stress induces age-dependent epigenetic changes in p11 gene expression in male mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cbb4ceffde4a4676832084b69846e2b8
work_keys_str_mv	AT mikyoungseo earlylifestressinducesagedependentepigeneticchangesinp11geneexpressioninmalemice AT junggoolee earlylifestressinducesagedependentepigeneticchangesinp11geneexpressioninmalemice AT sungwoopark earlylifestressinducesagedependentepigeneticchangesinp11geneexpressioninmalemice
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Early life stress induces age-dependent epigenetic changes in p11 gene expression in male mice

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