Nuclear restriction of HIV-1 infection by SUN1

Abstract Overexpression of the human Sad-1-Unc-84 homology protein 2 (SUN2) blocks HIV-1 infection in a capsid-dependent manner. In agreement, we showed that overexpression of SUN1 (Sad1 and UNC-84a) also blocks HIV-1 infection in a capsid-dependent manner. SUN2 and the related protein SUN1 are tran...

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Autores principales:	Mirjana Persaud, Anastasia Selyutina, Cindy Buffone, Silvana Opp, Daniel A. Donahue, Oliver Schwartz, Felipe Diaz-Griffero
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cbba42676a13485ba4ce7bc81eba44ec
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spelling	oai:doaj.org-article:cbba42676a13485ba4ce7bc81eba44ec2021-12-02T18:51:15ZNuclear restriction of HIV-1 infection by SUN110.1038/s41598-021-98541-42045-2322https://doaj.org/article/cbba42676a13485ba4ce7bc81eba44ec2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98541-4https://doaj.org/toc/2045-2322Abstract Overexpression of the human Sad-1-Unc-84 homology protein 2 (SUN2) blocks HIV-1 infection in a capsid-dependent manner. In agreement, we showed that overexpression of SUN1 (Sad1 and UNC-84a) also blocks HIV-1 infection in a capsid-dependent manner. SUN2 and the related protein SUN1 are transmembrane proteins located in the inner membrane of the nuclear envelope. The N-terminal domains of SUN1/2 localizes to the nucleoplasm while the C-terminal domains are localized in the nuclear lamina. Because the N-terminal domains of SUN1/2 are located in the nucleoplasm, we hypothesized that SUN1/2 might be interacting with the HIV-1 replication complex in the nucleus leading to HIV-1 inhibition. Our results demonstrated that SUN1/2 interacts with the HIV-1 capsid, and in agreement with our hypothesis, the use of N-terminal deletion mutants showed that SUN1/2 proteins bind to the viral capsid by using its N-terminal domain. SUN1/2 deletion mutants correlated restriction of HIV-1 with capsid binding. Interestingly, the ability of SUN1/2 to restrict HIV-1 also correlated with perinuclear localization of these proteins. In agreement with the notion that SUN proteins interact with the HIV-1 capsid in the nucleus, we found that restriction of HIV-1 by overexpression of SUN proteins do not block the entry of the HIV-1 core into the nucleus. Our results showed that HIV-1 restriction is mediated by the interaction of SUN1/2N-terminal domains with the HIV-1 core in the nuclear compartment.Mirjana PersaudAnastasia SelyutinaCindy BuffoneSilvana OppDaniel A. DonahueOliver SchwartzFelipe Diaz-GrifferoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mirjana Persaud Anastasia Selyutina Cindy Buffone Silvana Opp Daniel A. Donahue Oliver Schwartz Felipe Diaz-Griffero Nuclear restriction of HIV-1 infection by SUN1
description	Abstract Overexpression of the human Sad-1-Unc-84 homology protein 2 (SUN2) blocks HIV-1 infection in a capsid-dependent manner. In agreement, we showed that overexpression of SUN1 (Sad1 and UNC-84a) also blocks HIV-1 infection in a capsid-dependent manner. SUN2 and the related protein SUN1 are transmembrane proteins located in the inner membrane of the nuclear envelope. The N-terminal domains of SUN1/2 localizes to the nucleoplasm while the C-terminal domains are localized in the nuclear lamina. Because the N-terminal domains of SUN1/2 are located in the nucleoplasm, we hypothesized that SUN1/2 might be interacting with the HIV-1 replication complex in the nucleus leading to HIV-1 inhibition. Our results demonstrated that SUN1/2 interacts with the HIV-1 capsid, and in agreement with our hypothesis, the use of N-terminal deletion mutants showed that SUN1/2 proteins bind to the viral capsid by using its N-terminal domain. SUN1/2 deletion mutants correlated restriction of HIV-1 with capsid binding. Interestingly, the ability of SUN1/2 to restrict HIV-1 also correlated with perinuclear localization of these proteins. In agreement with the notion that SUN proteins interact with the HIV-1 capsid in the nucleus, we found that restriction of HIV-1 by overexpression of SUN proteins do not block the entry of the HIV-1 core into the nucleus. Our results showed that HIV-1 restriction is mediated by the interaction of SUN1/2N-terminal domains with the HIV-1 core in the nuclear compartment.
format	article
author	Mirjana Persaud Anastasia Selyutina Cindy Buffone Silvana Opp Daniel A. Donahue Oliver Schwartz Felipe Diaz-Griffero
author_facet	Mirjana Persaud Anastasia Selyutina Cindy Buffone Silvana Opp Daniel A. Donahue Oliver Schwartz Felipe Diaz-Griffero
author_sort	Mirjana Persaud
title	Nuclear restriction of HIV-1 infection by SUN1
title_short	Nuclear restriction of HIV-1 infection by SUN1
title_full	Nuclear restriction of HIV-1 infection by SUN1
title_fullStr	Nuclear restriction of HIV-1 infection by SUN1
title_full_unstemmed	Nuclear restriction of HIV-1 infection by SUN1
title_sort	nuclear restriction of hiv-1 infection by sun1
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cbba42676a13485ba4ce7bc81eba44ec
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Nuclear restriction of HIV-1 infection by SUN1

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