Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene

Abstract. Geraldi A, Tay CC, Ni’matuzahroh, Fatimah, Hanafi WNW. 2021. Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene. Biodiversitas 22: 4060-4066. This study is the first attempt at using the Next Generation Sequencing (NGS) method...

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Autores principales:	Almando Geraldi, Chia Chay Tay, Ni’matuzahroh Ni’matuzahroh, Fatimah FATIMAH, Wan Nurhayati Wan Hanafi
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	aquatic ecosystems hot spring bacteriome next generation sequencing 16s rrna Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cbbae462a6f14e20adeb3c6b8b5bce14
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spelling	oai:doaj.org-article:cbbae462a6f14e20adeb3c6b8b5bce142021-11-22T12:18:55ZUnraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene1412-033X2085-472210.13057/biodiv/d220955https://doaj.org/article/cbbae462a6f14e20adeb3c6b8b5bce142021-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8845https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Geraldi A, Tay CC, Ni’matuzahroh, Fatimah, Hanafi WNW. 2021. Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene. Biodiversitas 22: 4060-4066. This study is the first attempt at using the Next Generation Sequencing (NGS) method with 16S rRNA to understand the bacterial community structure in an Indonesian hot spring. This study aims to unravel the bacterial diversity of the Cangar Hot Spring as one of the most explored natural hot springs in East Java, Indonesia. We found Proteobacteria and Bacteroidetes as the two most abundant phyla. We discovered the first occurrence of genera Cloacibacterium and Methylobacillus in the hot spring ecosystem, which was the most dominant genera at Cangar Hot Spring. We also found several potential bacteria for bioindustry and bioremediation, such as Acinetobacter junii and Pseudomonas alcaligenes. Besides that, we also observed opportunistic pathogens from genera Comamonas and Vogesella. This study result will provide valuable information for further bioprospecting of bacteria with commercial potential and the development of health and safety measures in the Cangar Hot Spring, among others. Hopefully, this report would encourage the use of NGS technology for studying other hot springs in Indonesia.Almando GeraldiChia Chay TayNi’matuzahroh Ni’matuzahrohFatimah FATIMAHWan Nurhayati Wan HanafiMBI & UNS Soloarticleaquatic ecosystemshot springbacteriomenext generation sequencing16s rrnaBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aquatic ecosystems hot spring bacteriome next generation sequencing 16s rrna Biology (General) QH301-705.5
spellingShingle	aquatic ecosystems hot spring bacteriome next generation sequencing 16s rrna Biology (General) QH301-705.5 Almando Geraldi Chia Chay Tay Ni’matuzahroh Ni’matuzahroh Fatimah FATIMAH Wan Nurhayati Wan Hanafi Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene
description	Abstract. Geraldi A, Tay CC, Ni’matuzahroh, Fatimah, Hanafi WNW. 2021. Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene. Biodiversitas 22: 4060-4066. This study is the first attempt at using the Next Generation Sequencing (NGS) method with 16S rRNA to understand the bacterial community structure in an Indonesian hot spring. This study aims to unravel the bacterial diversity of the Cangar Hot Spring as one of the most explored natural hot springs in East Java, Indonesia. We found Proteobacteria and Bacteroidetes as the two most abundant phyla. We discovered the first occurrence of genera Cloacibacterium and Methylobacillus in the hot spring ecosystem, which was the most dominant genera at Cangar Hot Spring. We also found several potential bacteria for bioindustry and bioremediation, such as Acinetobacter junii and Pseudomonas alcaligenes. Besides that, we also observed opportunistic pathogens from genera Comamonas and Vogesella. This study result will provide valuable information for further bioprospecting of bacteria with commercial potential and the development of health and safety measures in the Cangar Hot Spring, among others. Hopefully, this report would encourage the use of NGS technology for studying other hot springs in Indonesia.
format	article
author	Almando Geraldi Chia Chay Tay Ni’matuzahroh Ni’matuzahroh Fatimah FATIMAH Wan Nurhayati Wan Hanafi
author_facet	Almando Geraldi Chia Chay Tay Ni’matuzahroh Ni’matuzahroh Fatimah FATIMAH Wan Nurhayati Wan Hanafi
author_sort	Almando Geraldi
title	Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene
title_short	Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene
title_full	Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene
title_fullStr	Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene
title_full_unstemmed	Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene
title_sort	unraveling the bacterial diversity of cangar hot spring, indonesia by next generation sequencing of 16s rrna gene
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/cbbae462a6f14e20adeb3c6b8b5bce14
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Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene

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