Suitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers

Weijie Chen, Sanyuan HuDepartment of General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, People&#39;s Republic of ChinaBackground: Particulate carriers are necessary to control the release of endostar and prolong its circulation in vivo. The purpose of this study...

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Autores principales:	Chen W, Hu S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cbbd6c40a08b4f7d80aee2115f577f52
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spelling	oai:doaj.org-article:cbbd6c40a08b4f7d80aee2115f577f522021-12-02T04:54:58ZSuitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers1176-91141178-2013https://doaj.org/article/cbbd6c40a08b4f7d80aee2115f577f522011-07-01T00:00:00Zhttp://www.dovepress.com/suitable-carriers-for-encapsulation-and-distribution-of-endostar-compa-a7938https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Weijie Chen, Sanyuan HuDepartment of General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, People&#39;s Republic of ChinaBackground: Particulate carriers are necessary to control the release of endostar and prolong its circulation in vivo. The purpose of this study was to identify a suitable carrier for the capsulation and delivery of endostar.Methods: We prepared a series of poly (DL-lactide-co-glycolide) (PLGA) and poly (ethylene glycol) (PEG)-modified PLGA (PEG-PLGA) particulate carriers, and then characterized them according to their ability to prolong the circulation of endostar, their physicochemical properties, endostar-loading content, and in vitro and in vivo particulate carrier release profiles.Results: All the particulate carriers had spherical core shell structures. The PEG-PLGA material and nanosize range appeared to enable the carriers to encapsulate more endostar, release endostar faster in vitro, and accumulate more endostar in vivo. The drug loading capacity of PEG-PLGA and PLGA nanoparticles was 8.03% &plusmn; 3.41% and 3.27% &plusmn; 5.26%, respectively, and for PEG-PLGA and PLGA microspheres was 15.32% &plusmn; 5.61% and 9.21% &plusmn; 4.73%. The cumulative amount of endostar released from the carriers in phosphate-buffered saline over 21 days was 23.79%, 20.45%, 15.13%, and 10.41%, respectively. Moreover, the terminal elimination half-life of endostar in the rabbit was 26.91 &plusmn; 7.93 hours and 9.32 &plusmn; 5.53 hours in the PEG-PLGA group and the PLGA nanoparticle group. Peak endostar concentration was reached at day 7 in the group treated with subcutaneous injection of PEG-PLGA microspheres and at day 14 in the group receiving subcutaneous injection of PLGA microspheres. Endostar was detectable in vivo in both groups after injection of the particulate carriers.Conclusion: PEG-PLGA nanoparticles might be better than other nanoparticulate carriers for encapsulation and distribution of endostar.Keywords: poly(DL-lactide-co-glycolide), nanoparticle, microsphere, endostar, peptide deliveryChen WHu SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1535-1541 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen W Hu S Suitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers
description	Weijie Chen, Sanyuan HuDepartment of General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, People&#39;s Republic of ChinaBackground: Particulate carriers are necessary to control the release of endostar and prolong its circulation in vivo. The purpose of this study was to identify a suitable carrier for the capsulation and delivery of endostar.Methods: We prepared a series of poly (DL-lactide-co-glycolide) (PLGA) and poly (ethylene glycol) (PEG)-modified PLGA (PEG-PLGA) particulate carriers, and then characterized them according to their ability to prolong the circulation of endostar, their physicochemical properties, endostar-loading content, and in vitro and in vivo particulate carrier release profiles.Results: All the particulate carriers had spherical core shell structures. The PEG-PLGA material and nanosize range appeared to enable the carriers to encapsulate more endostar, release endostar faster in vitro, and accumulate more endostar in vivo. The drug loading capacity of PEG-PLGA and PLGA nanoparticles was 8.03% &plusmn; 3.41% and 3.27% &plusmn; 5.26%, respectively, and for PEG-PLGA and PLGA microspheres was 15.32% &plusmn; 5.61% and 9.21% &plusmn; 4.73%. The cumulative amount of endostar released from the carriers in phosphate-buffered saline over 21 days was 23.79%, 20.45%, 15.13%, and 10.41%, respectively. Moreover, the terminal elimination half-life of endostar in the rabbit was 26.91 &plusmn; 7.93 hours and 9.32 &plusmn; 5.53 hours in the PEG-PLGA group and the PLGA nanoparticle group. Peak endostar concentration was reached at day 7 in the group treated with subcutaneous injection of PEG-PLGA microspheres and at day 14 in the group receiving subcutaneous injection of PLGA microspheres. Endostar was detectable in vivo in both groups after injection of the particulate carriers.Conclusion: PEG-PLGA nanoparticles might be better than other nanoparticulate carriers for encapsulation and distribution of endostar.Keywords: poly(DL-lactide-co-glycolide), nanoparticle, microsphere, endostar, peptide delivery
format	article
author	Chen W Hu S
author_facet	Chen W Hu S
author_sort	Chen W
title	Suitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers
title_short	Suitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers
title_full	Suitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers
title_fullStr	Suitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers
title_full_unstemmed	Suitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers
title_sort	suitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/cbbd6c40a08b4f7d80aee2115f577f52
work_keys_str_mv	AT chenw suitablecarriersforencapsulationanddistributionofendostarcomparisonofendostarloadedparticulatecarriers AT hus suitablecarriersforencapsulationanddistributionofendostarcomparisonofendostarloadedparticulatecarriers
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Suitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers

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