Synapsis and meiotic recombination in male Chinese muntjac (Muntiacus reevesi).

The muntjacs (Muntiacus, Cervidae) have been extensively studied in terms of chromosomal and karyotypic evolution. However, little is known about their meiotic chromosomes particularly the recombination patterns of homologous chromosomes. We used immunostained surface spreads to visualise synaptonem...

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Autores principales:	Qingling Yang, Ding Zhang, Mei Leng, Ling Yang, Liangwen Zhong, Howard J Cooke, Qinghua Shi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cbbf1a77d74d4518a62681da963a4874
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spelling	oai:doaj.org-article:cbbf1a77d74d4518a62681da963a48742021-11-18T06:54:46ZSynapsis and meiotic recombination in male Chinese muntjac (Muntiacus reevesi).1932-620310.1371/journal.pone.0019255https://doaj.org/article/cbbf1a77d74d4518a62681da963a48742011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21559438/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The muntjacs (Muntiacus, Cervidae) have been extensively studied in terms of chromosomal and karyotypic evolution. However, little is known about their meiotic chromosomes particularly the recombination patterns of homologous chromosomes. We used immunostained surface spreads to visualise synaptonemal complexes (SCs), recombination foci and kinetochores with antibodies against marker proteins. As in other mammals pachytene was the longest stage of meiotic prophase. 39.4% of XY bivalents lacked MLH1 foci compared to less than 0.5% of autosomes. The average number of MLH1 foci per pachytene cell in M. reevesi was 29.8. The distribution of MLH1 foci differed from other mammals. On SCs with one focus, the distribution was more even in M. reevesi than in other mammals; for SCs that have two or more MLH1 foci, usually there was a larger peak in the sub-centromere region than other regions on SC in M. reevesi. Additionally, there was a lower level of interference between foci in M. reevesi than in mouse or human. These observations may suggest that the regulation of homologous recombination in M. reevesi is slightly different from other mammals and will improve our understanding of the regulation of meiotic recombination, with respect to recombination frequency and position.Qingling YangDing ZhangMei LengLing YangLiangwen ZhongHoward J CookeQinghua ShiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e19255 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qingling Yang Ding Zhang Mei Leng Ling Yang Liangwen Zhong Howard J Cooke Qinghua Shi Synapsis and meiotic recombination in male Chinese muntjac (Muntiacus reevesi).
description	The muntjacs (Muntiacus, Cervidae) have been extensively studied in terms of chromosomal and karyotypic evolution. However, little is known about their meiotic chromosomes particularly the recombination patterns of homologous chromosomes. We used immunostained surface spreads to visualise synaptonemal complexes (SCs), recombination foci and kinetochores with antibodies against marker proteins. As in other mammals pachytene was the longest stage of meiotic prophase. 39.4% of XY bivalents lacked MLH1 foci compared to less than 0.5% of autosomes. The average number of MLH1 foci per pachytene cell in M. reevesi was 29.8. The distribution of MLH1 foci differed from other mammals. On SCs with one focus, the distribution was more even in M. reevesi than in other mammals; for SCs that have two or more MLH1 foci, usually there was a larger peak in the sub-centromere region than other regions on SC in M. reevesi. Additionally, there was a lower level of interference between foci in M. reevesi than in mouse or human. These observations may suggest that the regulation of homologous recombination in M. reevesi is slightly different from other mammals and will improve our understanding of the regulation of meiotic recombination, with respect to recombination frequency and position.
format	article
author	Qingling Yang Ding Zhang Mei Leng Ling Yang Liangwen Zhong Howard J Cooke Qinghua Shi
author_facet	Qingling Yang Ding Zhang Mei Leng Ling Yang Liangwen Zhong Howard J Cooke Qinghua Shi
author_sort	Qingling Yang
title	Synapsis and meiotic recombination in male Chinese muntjac (Muntiacus reevesi).
title_short	Synapsis and meiotic recombination in male Chinese muntjac (Muntiacus reevesi).
title_full	Synapsis and meiotic recombination in male Chinese muntjac (Muntiacus reevesi).
title_fullStr	Synapsis and meiotic recombination in male Chinese muntjac (Muntiacus reevesi).
title_full_unstemmed	Synapsis and meiotic recombination in male Chinese muntjac (Muntiacus reevesi).
title_sort	synapsis and meiotic recombination in male chinese muntjac (muntiacus reevesi).
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/cbbf1a77d74d4518a62681da963a4874
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Synapsis and meiotic recombination in male Chinese muntjac (Muntiacus reevesi).

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