Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin

Modified citrus pectin (MCP), a commercially available dietary supplement prepared from citrus pectin, contains several different polysaccharide domains, but its primary chemical structure and the binding epitopes that antagonize galectin-3 function remain unclear. In this study, five fractions were...

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Autores principales:	Tao Zhang, Guoqing Sun, Ming Shuai, Jingyu Ye, Jian Huang, Xiaodong Yao, Chengxin Sun, Xun Min
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Modified citrus pectin Polygalacturonase hydrolysis Purification Chemical analysis Galectin-3 inhibitor Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/cbc8fe2acec14da8b47ecaf0b61ea651
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spelling	oai:doaj.org-article:cbc8fe2acec14da8b47ecaf0b61ea6512021-11-26T04:39:48ZPurification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin2590-157510.1016/j.fochx.2021.100169https://doaj.org/article/cbc8fe2acec14da8b47ecaf0b61ea6512021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590157521000572https://doaj.org/toc/2590-1575Modified citrus pectin (MCP), a commercially available dietary supplement prepared from citrus pectin, contains several different polysaccharide domains, but its primary chemical structure and the binding epitopes that antagonize galectin-3 function remain unclear. In this study, five fractions were isolated from MCP after endo-polygalacturonase degradation (EMCP) and a combination of DEAE-cellulose and Sepharose CL-6B or Sephadex G-75 chromatography. Their primary structures, abilities to inhibit galectin-3-mediated hemagglutination, and antiproliferation activities on MCF-7 and A549 cell lines were studied. Results showed that EMCP-3p, one of the five fractions, was composed of Glc (89.8%), Gal (3.8%), Ara (3.1%), GalA (1.1%), Man (0.9%), and Rha (1.3%) with an average molecular weight of 88.4 KDa, which had the most substantial degree of galectin-3 inhibition with an MIC of 31.25 μg/mL, and it exhibited remarkable cytotoxicity against MCF-7 (36.7%) and A549 (57.4%) cell lines. These results provide new insight into the structure–function relationships of EMCP-derived polysaccharides.Tao ZhangGuoqing SunMing ShuaiJingyu YeJian HuangXiaodong YaoChengxin SunXun MinElsevierarticleModified citrus pectinPolygalacturonase hydrolysisPurificationChemical analysisGalectin-3 inhibitorNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENFood Chemistry: X, Vol 12, Iss , Pp 100169- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Modified citrus pectin Polygalacturonase hydrolysis Purification Chemical analysis Galectin-3 inhibitor Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
spellingShingle	Modified citrus pectin Polygalacturonase hydrolysis Purification Chemical analysis Galectin-3 inhibitor Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Tao Zhang Guoqing Sun Ming Shuai Jingyu Ye Jian Huang Xiaodong Yao Chengxin Sun Xun Min Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin
description	Modified citrus pectin (MCP), a commercially available dietary supplement prepared from citrus pectin, contains several different polysaccharide domains, but its primary chemical structure and the binding epitopes that antagonize galectin-3 function remain unclear. In this study, five fractions were isolated from MCP after endo-polygalacturonase degradation (EMCP) and a combination of DEAE-cellulose and Sepharose CL-6B or Sephadex G-75 chromatography. Their primary structures, abilities to inhibit galectin-3-mediated hemagglutination, and antiproliferation activities on MCF-7 and A549 cell lines were studied. Results showed that EMCP-3p, one of the five fractions, was composed of Glc (89.8%), Gal (3.8%), Ara (3.1%), GalA (1.1%), Man (0.9%), and Rha (1.3%) with an average molecular weight of 88.4 KDa, which had the most substantial degree of galectin-3 inhibition with an MIC of 31.25 μg/mL, and it exhibited remarkable cytotoxicity against MCF-7 (36.7%) and A549 (57.4%) cell lines. These results provide new insight into the structure–function relationships of EMCP-derived polysaccharides.
format	article
author	Tao Zhang Guoqing Sun Ming Shuai Jingyu Ye Jian Huang Xiaodong Yao Chengxin Sun Xun Min
author_facet	Tao Zhang Guoqing Sun Ming Shuai Jingyu Ye Jian Huang Xiaodong Yao Chengxin Sun Xun Min
author_sort	Tao Zhang
title	Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin
title_short	Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin
title_full	Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin
title_fullStr	Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin
title_full_unstemmed	Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin
title_sort	purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic ph-modified citrus pectin
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/cbc8fe2acec14da8b47ecaf0b61ea651
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Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin

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