TRPV6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency

Summary: The precise effect of estrogen (E2) on osteoclast function is still poorly understood. The aim of this study was to investigate the potential role of transient receptor potential vanilloid 6 (TRPV6) in E2-mediated osteoclast function and to characterize the relevant underlying mechanisms. H...

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Autores principales:	Jun Ma, Jiajia Lu, Zhibin Zhou, Nan Lu, Jia He, Lei Zhu, Tianwen Ye
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Molecular biology Endocrinology Developmental biology Science Q
Acceso en línea:	https://doaj.org/article/cbca6f0d021a4b45a58607d0ee3b1ff1
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spelling	oai:doaj.org-article:cbca6f0d021a4b45a58607d0ee3b1ff12021-11-20T05:08:59ZTRPV6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency2589-004210.1016/j.isci.2021.103261https://doaj.org/article/cbca6f0d021a4b45a58607d0ee3b1ff12021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S258900422101230Xhttps://doaj.org/toc/2589-0042Summary: The precise effect of estrogen (E2) on osteoclast function is still poorly understood. The aim of this study was to investigate the potential role of transient receptor potential vanilloid 6 (TRPV6) in E2-mediated osteoclast function and to characterize the relevant underlying mechanisms. Here, we found that Trpv6 is drastically decreased in ovariectomy operation animals and the administration of E2 results in an increased expression of Trpv6 in osteoclasts. In contrast, Trpv6 depletion significantly blocked the inhibitory effects of E2 on bone resorption activity, and silencing Trpv6 alleviated E2-induced osteoclast apoptosis. In addition, we found that E2 regulates the transcription of Trpv6 through ERα, by interacting with C/EBPβ and NF-κB. Chip assay analysis indicated that C/EBPβ regulates Trpv6 transcription by binding to Trpv6 promoter fragments −1,866 nt to −1,761 nt and −2,685 nt to −2,580 nt, whereas NF-κB binds to the −953 nt to −851 nt region. We conclude that TRPV6 has a significant effect on E2-mediated osteoclast function.Jun MaJiajia LuZhibin ZhouNan LuJia HeLei ZhuTianwen YeElsevierarticleMolecular biologyEndocrinologyDevelopmental biologyScienceQENiScience, Vol 24, Iss 11, Pp 103261- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Molecular biology Endocrinology Developmental biology Science Q
spellingShingle	Molecular biology Endocrinology Developmental biology Science Q Jun Ma Jiajia Lu Zhibin Zhou Nan Lu Jia He Lei Zhu Tianwen Ye TRPV6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency
description	Summary: The precise effect of estrogen (E2) on osteoclast function is still poorly understood. The aim of this study was to investigate the potential role of transient receptor potential vanilloid 6 (TRPV6) in E2-mediated osteoclast function and to characterize the relevant underlying mechanisms. Here, we found that Trpv6 is drastically decreased in ovariectomy operation animals and the administration of E2 results in an increased expression of Trpv6 in osteoclasts. In contrast, Trpv6 depletion significantly blocked the inhibitory effects of E2 on bone resorption activity, and silencing Trpv6 alleviated E2-induced osteoclast apoptosis. In addition, we found that E2 regulates the transcription of Trpv6 through ERα, by interacting with C/EBPβ and NF-κB. Chip assay analysis indicated that C/EBPβ regulates Trpv6 transcription by binding to Trpv6 promoter fragments −1,866 nt to −1,761 nt and −2,685 nt to −2,580 nt, whereas NF-κB binds to the −953 nt to −851 nt region. We conclude that TRPV6 has a significant effect on E2-mediated osteoclast function.
format	article
author	Jun Ma Jiajia Lu Zhibin Zhou Nan Lu Jia He Lei Zhu Tianwen Ye
author_facet	Jun Ma Jiajia Lu Zhibin Zhou Nan Lu Jia He Lei Zhu Tianwen Ye
author_sort	Jun Ma
title	TRPV6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency
title_short	TRPV6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency
title_full	TRPV6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency
title_fullStr	TRPV6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency
title_full_unstemmed	TRPV6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency
title_sort	trpv6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/cbca6f0d021a4b45a58607d0ee3b1ff1
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TRPV6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency

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