Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta

Abstract Trophoblast stem (TS) cells in the mouse derive from the polar trophectoderm of the blastocyst and persist through early gestation (to E8.5) to support placental development. Further development and growth is proposed to rely on layer-restricted progenitor cells. Stem cell antigen (Sca) -1...

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Autores principales:	Bryony V. Natale, Christina Schweitzer, Martha Hughes, Maria A. Globisch, Ramie Kotadia, Emilie Tremblay, Priscilla Vu, James C. Cross, David R. C. Natale
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cbd388ee15dd480eb87993b77afa4930
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spelling	oai:doaj.org-article:cbd388ee15dd480eb87993b77afa49302021-12-02T15:05:41ZSca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta10.1038/s41598-017-06008-22045-2322https://doaj.org/article/cbd388ee15dd480eb87993b77afa49302017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06008-2https://doaj.org/toc/2045-2322Abstract Trophoblast stem (TS) cells in the mouse derive from the polar trophectoderm of the blastocyst and persist through early gestation (to E8.5) to support placental development. Further development and growth is proposed to rely on layer-restricted progenitor cells. Stem cell antigen (Sca) -1 is a member of the Ly6 gene family and a known marker of stem cells in both hematopoietic and non-hematopoietic mouse tissues. Having identified that Sca-1 mRNA was highly expressed in mouse TS cells in culture, we found that it was also expressed in a subset of trophoblast within the chorion and labyrinth layer of the mouse placenta. Isolation and in vitro culture of Sca-1+ trophoblast cells from both differentiated TS cell cultures and dissected mouse placentae resulted in proliferating colonies that expressed known markers of TS cells. Furthermore, these cells could be stimulated to differentiate and expressed markers of both junctional zone and labyrinth trophoblast subtypes in a manner comparable to established mouse TS cell lines. Our results suggest that we have identified a subpopulation of TS cell-like cells that persist in the mid- to late- gestation mouse placenta as well as a cell surface protein that can be used to identify and isolate these cells.Bryony V. NataleChristina SchweitzerMartha HughesMaria A. GlobischRamie KotadiaEmilie TremblayPriscilla VuJames C. CrossDavid R. C. NataleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bryony V. Natale Christina Schweitzer Martha Hughes Maria A. Globisch Ramie Kotadia Emilie Tremblay Priscilla Vu James C. Cross David R. C. Natale Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta
description	Abstract Trophoblast stem (TS) cells in the mouse derive from the polar trophectoderm of the blastocyst and persist through early gestation (to E8.5) to support placental development. Further development and growth is proposed to rely on layer-restricted progenitor cells. Stem cell antigen (Sca) -1 is a member of the Ly6 gene family and a known marker of stem cells in both hematopoietic and non-hematopoietic mouse tissues. Having identified that Sca-1 mRNA was highly expressed in mouse TS cells in culture, we found that it was also expressed in a subset of trophoblast within the chorion and labyrinth layer of the mouse placenta. Isolation and in vitro culture of Sca-1+ trophoblast cells from both differentiated TS cell cultures and dissected mouse placentae resulted in proliferating colonies that expressed known markers of TS cells. Furthermore, these cells could be stimulated to differentiate and expressed markers of both junctional zone and labyrinth trophoblast subtypes in a manner comparable to established mouse TS cell lines. Our results suggest that we have identified a subpopulation of TS cell-like cells that persist in the mid- to late- gestation mouse placenta as well as a cell surface protein that can be used to identify and isolate these cells.
format	article
author	Bryony V. Natale Christina Schweitzer Martha Hughes Maria A. Globisch Ramie Kotadia Emilie Tremblay Priscilla Vu James C. Cross David R. C. Natale
author_facet	Bryony V. Natale Christina Schweitzer Martha Hughes Maria A. Globisch Ramie Kotadia Emilie Tremblay Priscilla Vu James C. Cross David R. C. Natale
author_sort	Bryony V. Natale
title	Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta
title_short	Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta
title_full	Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta
title_fullStr	Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta
title_full_unstemmed	Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta
title_sort	sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cbd388ee15dd480eb87993b77afa4930
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Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta

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