Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort.

MicroRNA (miRNA) circulating in plasma have been proposed as biomarkers for a variety of conditions and diseases, including complications during pregnancy. During pregnancy, about 15-25% of maternal plasma exosomes, a small size-class of EVs, are hypothesized to originate in the placenta, and may pl...

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Autores principales: Helen Bermudez Foley, Caitlin G Howe, Sandrah P Eckel, Thomas Chavez, Lili Gevorkian, Eileen Granada Reyes, Bethany Kapanke, Danilo Martinez, Shanyan Xue, Shakira F Suglia, Theresa M Bastain, Carmen Marsit, Carrie V Breton
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Publicado: Public Library of Science (PLoS) 2021
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Acceso en línea:https://doaj.org/article/cbda2d27b537441f9f35ab7db7df2c12
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spelling oai:doaj.org-article:cbda2d27b537441f9f35ab7db7df2c122021-12-02T20:05:40ZExtracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort.1932-620310.1371/journal.pone.0251259https://doaj.org/article/cbda2d27b537441f9f35ab7db7df2c122021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251259https://doaj.org/toc/1932-6203MicroRNA (miRNA) circulating in plasma have been proposed as biomarkers for a variety of conditions and diseases, including complications during pregnancy. During pregnancy, about 15-25% of maternal plasma exosomes, a small size-class of EVs, are hypothesized to originate in the placenta, and may play a role in communication between the fetus and mother. However, few studies have addressed changes in miRNA over the course of pregnancy with repeated measures, nor focused on diverse populations. We describe changes in miRNA in early and late pregnancy from the MADRES cohort of primarily low-income Hispanic women based in Los Angeles, CA. miRNA derived from extracellular-vesicles (EVs) were isolated from maternal blood plasma samples collected in early and late pregnancy. In this study, we identified 64 of 130 detectable miRNA which significantly increased with gestational age at the time of collection (GA), and 26 which decreased with GA. Possible fetal sex-specific associations were observed for 30 of these 90 significant miRNA. Predicted gene targets for miRNA significantly associated with GA were identified using MirDIP and were found to be enriched for Gene Ontology categories that included energetic and metabolic processes but were underrepresented in immune-related categories. Circulating EV-associated miRNA during pregnancy are likely important for maternal-fetal communication, and may play roles in supporting and maintaining a healthy pregnancy, given the changing needs of the fetus.Helen Bermudez FoleyCaitlin G HoweSandrah P EckelThomas ChavezLili GevorkianEileen Granada ReyesBethany KapankeDanilo MartinezShanyan XueShakira F SugliaTheresa M BastainCarmen MarsitCarrie V BretonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251259 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Helen Bermudez Foley
Caitlin G Howe
Sandrah P Eckel
Thomas Chavez
Lili Gevorkian
Eileen Granada Reyes
Bethany Kapanke
Danilo Martinez
Shanyan Xue
Shakira F Suglia
Theresa M Bastain
Carmen Marsit
Carrie V Breton
Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort.
description MicroRNA (miRNA) circulating in plasma have been proposed as biomarkers for a variety of conditions and diseases, including complications during pregnancy. During pregnancy, about 15-25% of maternal plasma exosomes, a small size-class of EVs, are hypothesized to originate in the placenta, and may play a role in communication between the fetus and mother. However, few studies have addressed changes in miRNA over the course of pregnancy with repeated measures, nor focused on diverse populations. We describe changes in miRNA in early and late pregnancy from the MADRES cohort of primarily low-income Hispanic women based in Los Angeles, CA. miRNA derived from extracellular-vesicles (EVs) were isolated from maternal blood plasma samples collected in early and late pregnancy. In this study, we identified 64 of 130 detectable miRNA which significantly increased with gestational age at the time of collection (GA), and 26 which decreased with GA. Possible fetal sex-specific associations were observed for 30 of these 90 significant miRNA. Predicted gene targets for miRNA significantly associated with GA were identified using MirDIP and were found to be enriched for Gene Ontology categories that included energetic and metabolic processes but were underrepresented in immune-related categories. Circulating EV-associated miRNA during pregnancy are likely important for maternal-fetal communication, and may play roles in supporting and maintaining a healthy pregnancy, given the changing needs of the fetus.
format article
author Helen Bermudez Foley
Caitlin G Howe
Sandrah P Eckel
Thomas Chavez
Lili Gevorkian
Eileen Granada Reyes
Bethany Kapanke
Danilo Martinez
Shanyan Xue
Shakira F Suglia
Theresa M Bastain
Carmen Marsit
Carrie V Breton
author_facet Helen Bermudez Foley
Caitlin G Howe
Sandrah P Eckel
Thomas Chavez
Lili Gevorkian
Eileen Granada Reyes
Bethany Kapanke
Danilo Martinez
Shanyan Xue
Shakira F Suglia
Theresa M Bastain
Carmen Marsit
Carrie V Breton
author_sort Helen Bermudez Foley
title Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort.
title_short Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort.
title_full Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort.
title_fullStr Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort.
title_full_unstemmed Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort.
title_sort extracellular vesicle-enriched mirna profiles across pregnancy in the madres cohort.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/cbda2d27b537441f9f35ab7db7df2c12
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