Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network

Climate change has been associated with a higher incidence of combined adverse environmental conditions that can promote a significant decrease in crop productivity. However, knowledge on how a combination of stresses might affect plant development is still scarce. MicroRNAs (miRNAs) have been propo...

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Autores principales:	Pascual Villalba-Bermell, Joan Marquez-Molins, María-Carmen Marques, Andrea G. Hernandez-Azurdia, Julia Corell-Sierra, Belén Picó, Antonio J. Monforte, Santiago F. Elena, Gustavo G. Gomez
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	crop production and climate change miRNAs and stress response in Cucumis melo RNA regulatory networks RNA-seq and systems biology biotic and abiotic stress Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/cbdb6775997a4596a07f5cdd1f201a5d
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spelling	oai:doaj.org-article:cbdb6775997a4596a07f5cdd1f201a5d2021-12-01T02:38:00ZCombined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network1664-462X10.3389/fpls.2021.769093https://doaj.org/article/cbdb6775997a4596a07f5cdd1f201a5d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.769093/fullhttps://doaj.org/toc/1664-462XClimate change has been associated with a higher incidence of combined adverse environmental conditions that can promote a significant decrease in crop productivity. However, knowledge on how a combination of stresses might affect plant development is still scarce. MicroRNAs (miRNAs) have been proposed as potential targets for improving crop productivity. Here, we have combined deep-sequencing, computational characterization of responsive miRNAs and validation of their regulatory role in a comprehensive analysis of response of melon to several combinations of four stresses (cold, salinity, short day, and infection with a fungus). Twenty-two miRNA families responding to double and/or triple stresses were identified. The regulatory role of the differentially expressed miRNAs was validated by quantitative measurements of the expression of the corresponding target genes. A high proportion (ca. 60%) of these families (mainly highly conserved miRNAs targeting transcription factors) showed a non-additive response to multiple stresses in comparison with that observed under each one of the stresses individually. Among those miRNAs showing non-additive response to stress combinations, most interactions were negative, suggesting the existence of functional convergence in the miRNA-mediated response to combined stresses. Taken together, our results provide compelling pieces of evidence that the response to combined stresses cannot be easily predicted from the study individual stresses.Pascual Villalba-BermellJoan Marquez-MolinsMaría-Carmen MarquesAndrea G. Hernandez-AzurdiaJulia Corell-SierraBelén PicóAntonio J. MonforteSantiago F. ElenaSantiago F. ElenaGustavo G. GomezFrontiers Media S.A.articlecrop production and climate changemiRNAs and stress response in Cucumis meloRNA regulatory networksRNA-seq and systems biologybiotic and abiotic stressPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	crop production and climate change miRNAs and stress response in Cucumis melo RNA regulatory networks RNA-seq and systems biology biotic and abiotic stress Plant culture SB1-1110
spellingShingle	crop production and climate change miRNAs and stress response in Cucumis melo RNA regulatory networks RNA-seq and systems biology biotic and abiotic stress Plant culture SB1-1110 Pascual Villalba-Bermell Joan Marquez-Molins María-Carmen Marques Andrea G. Hernandez-Azurdia Julia Corell-Sierra Belén Picó Antonio J. Monforte Santiago F. Elena Santiago F. Elena Gustavo G. Gomez Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network
description	Climate change has been associated with a higher incidence of combined adverse environmental conditions that can promote a significant decrease in crop productivity. However, knowledge on how a combination of stresses might affect plant development is still scarce. MicroRNAs (miRNAs) have been proposed as potential targets for improving crop productivity. Here, we have combined deep-sequencing, computational characterization of responsive miRNAs and validation of their regulatory role in a comprehensive analysis of response of melon to several combinations of four stresses (cold, salinity, short day, and infection with a fungus). Twenty-two miRNA families responding to double and/or triple stresses were identified. The regulatory role of the differentially expressed miRNAs was validated by quantitative measurements of the expression of the corresponding target genes. A high proportion (ca. 60%) of these families (mainly highly conserved miRNAs targeting transcription factors) showed a non-additive response to multiple stresses in comparison with that observed under each one of the stresses individually. Among those miRNAs showing non-additive response to stress combinations, most interactions were negative, suggesting the existence of functional convergence in the miRNA-mediated response to combined stresses. Taken together, our results provide compelling pieces of evidence that the response to combined stresses cannot be easily predicted from the study individual stresses.
format	article
author	Pascual Villalba-Bermell Joan Marquez-Molins María-Carmen Marques Andrea G. Hernandez-Azurdia Julia Corell-Sierra Belén Picó Antonio J. Monforte Santiago F. Elena Santiago F. Elena Gustavo G. Gomez
author_facet	Pascual Villalba-Bermell Joan Marquez-Molins María-Carmen Marques Andrea G. Hernandez-Azurdia Julia Corell-Sierra Belén Picó Antonio J. Monforte Santiago F. Elena Santiago F. Elena Gustavo G. Gomez
author_sort	Pascual Villalba-Bermell
title	Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network
title_short	Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network
title_full	Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network
title_fullStr	Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network
title_full_unstemmed	Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network
title_sort	combined stress conditions in melon induce non-additive effects in the core mirna regulatory network
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/cbdb6775997a4596a07f5cdd1f201a5d
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Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network

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