Oxidative nanopatterning of titanium generates mesoporous surfaces with antimicrobial properties

Fabio Variola,1,2 Sylvia Francis Zalzal,3 Annie Leduc,3 Jean Barbeau,3 Antonio Nanci31Faculty of Engineering, Department of Mechanical Engineering, 2Faculty of Science, Department of Physics, University of Ottawa, Ottawa, ON, 3Faculty of Dental Medicine, Université de Montréal...

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Autores principales:	Variola F, Zalzal SF, Leduc A, Barbeau J, Nanci A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cbe1329f0e894842a29f393b9cdfc9ea
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spelling	oai:doaj.org-article:cbe1329f0e894842a29f393b9cdfc9ea2021-12-02T02:04:16ZOxidative nanopatterning of titanium generates mesoporous surfaces with antimicrobial properties1178-2013https://doaj.org/article/cbe1329f0e894842a29f393b9cdfc9ea2014-05-01T00:00:00Zhttp://www.dovepress.com/oxidative-nanopatterning-of-titanium-generates-mesoporous-surfaces-wit-a16801https://doaj.org/toc/1178-2013 Fabio Variola,1,2 Sylvia Francis Zalzal,3 Annie Leduc,3 Jean Barbeau,3 Antonio Nanci31Faculty of Engineering, Department of Mechanical Engineering, 2Faculty of Science, Department of Physics, University of Ottawa, Ottawa, ON, 3Faculty of Dental Medicine, Université de Montréal, Montreal, QC, CanadaAbstract: Mesoporous surfaces generated by oxidative nanopatterning have the capacity to selectively regulate cell behavior, but their impact on microorganisms has not yet been explored. The main objective of this study was to test the effects of such surfaces on the adherence of two common bacteria and one yeast strain that are responsible for nosocomial infections in clinical settings and biomedical applications. In addition, because surface characteristics are known to affect bacterial adhesion, we further characterized the physicochemical properties of the mesoporous surfaces. Focused ion beam (FIB) was used to generate ultrathin sections for elemental analysis by energy-dispersive X-ray spectroscopy (EDS), nanobeam electron diffraction (NBED), and high-angle annular dark field (HAADF) scanning transmission electron microscopy (STEM) imaging. The adherence of Staphylococcus aureus, Escherichia coli and Candida albicans onto titanium disks with mesoporous and polished surfaces was compared. Disks with the two surfaces side-by-side were also used for direct visual comparison. Qualitative and quantitative results from this study indicate that bacterial adhesion is significantly hindered by the mesoporous surface. In addition, we provide evidence that it alters structural parameters of C. albicans that determine its invasiveness potential, suggesting that microorganisms can sense and respond to the mesoporous surface. Our findings demonstrate the efficiency of a simple chemical oxidative treatment in generating nanotextured surfaces with antimicrobial capacity with potential applications in the implant manufacturing industry and hospital setting.Keywords: mesoporosity, surface characterization, microorganisms, adhesionVariola FZalzal SFLeduc ABarbeau JNanci ADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2319-2325 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Variola F Zalzal SF Leduc A Barbeau J Nanci A Oxidative nanopatterning of titanium generates mesoporous surfaces with antimicrobial properties
description	Fabio Variola,1,2 Sylvia Francis Zalzal,3 Annie Leduc,3 Jean Barbeau,3 Antonio Nanci31Faculty of Engineering, Department of Mechanical Engineering, 2Faculty of Science, Department of Physics, University of Ottawa, Ottawa, ON, 3Faculty of Dental Medicine, Université de Montréal, Montreal, QC, CanadaAbstract: Mesoporous surfaces generated by oxidative nanopatterning have the capacity to selectively regulate cell behavior, but their impact on microorganisms has not yet been explored. The main objective of this study was to test the effects of such surfaces on the adherence of two common bacteria and one yeast strain that are responsible for nosocomial infections in clinical settings and biomedical applications. In addition, because surface characteristics are known to affect bacterial adhesion, we further characterized the physicochemical properties of the mesoporous surfaces. Focused ion beam (FIB) was used to generate ultrathin sections for elemental analysis by energy-dispersive X-ray spectroscopy (EDS), nanobeam electron diffraction (NBED), and high-angle annular dark field (HAADF) scanning transmission electron microscopy (STEM) imaging. The adherence of Staphylococcus aureus, Escherichia coli and Candida albicans onto titanium disks with mesoporous and polished surfaces was compared. Disks with the two surfaces side-by-side were also used for direct visual comparison. Qualitative and quantitative results from this study indicate that bacterial adhesion is significantly hindered by the mesoporous surface. In addition, we provide evidence that it alters structural parameters of C. albicans that determine its invasiveness potential, suggesting that microorganisms can sense and respond to the mesoporous surface. Our findings demonstrate the efficiency of a simple chemical oxidative treatment in generating nanotextured surfaces with antimicrobial capacity with potential applications in the implant manufacturing industry and hospital setting.Keywords: mesoporosity, surface characterization, microorganisms, adhesion
format	article
author	Variola F Zalzal SF Leduc A Barbeau J Nanci A
author_facet	Variola F Zalzal SF Leduc A Barbeau J Nanci A
author_sort	Variola F
title	Oxidative nanopatterning of titanium generates mesoporous surfaces with antimicrobial properties
title_short	Oxidative nanopatterning of titanium generates mesoporous surfaces with antimicrobial properties
title_full	Oxidative nanopatterning of titanium generates mesoporous surfaces with antimicrobial properties
title_fullStr	Oxidative nanopatterning of titanium generates mesoporous surfaces with antimicrobial properties
title_full_unstemmed	Oxidative nanopatterning of titanium generates mesoporous surfaces with antimicrobial properties
title_sort	oxidative nanopatterning of titanium generates mesoporous surfaces with antimicrobial properties
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/cbe1329f0e894842a29f393b9cdfc9ea
work_keys_str_mv	AT variolaf oxidativenanopatterningoftitaniumgeneratesmesoporoussurfaceswithantimicrobialproperties AT zalzalsf oxidativenanopatterningoftitaniumgeneratesmesoporoussurfaceswithantimicrobialproperties AT leduca oxidativenanopatterningoftitaniumgeneratesmesoporoussurfaceswithantimicrobialproperties AT barbeauj oxidativenanopatterningoftitaniumgeneratesmesoporoussurfaceswithantimicrobialproperties AT nancia oxidativenanopatterningoftitaniumgeneratesmesoporoussurfaceswithantimicrobialproperties
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Oxidative nanopatterning of titanium generates mesoporous surfaces with antimicrobial properties

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