Antifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from <i>Pichia norvegensis</i> Grown on Tofu Wastewater

Yeast can be isolated from tofu wastewater and the cell wall in the form of β-glucan can act as a natural decontaminant agent. This study aimed to isolate and characterize native yeast from tofu wastewater, which can be extracted to obtain β-glucan and then identify the yeast and its β-glucan activi...

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Autores principales:	Gemilang Lara Utama, Mahardhika Puspa Arum Suraloka, Tita Rialita, Roostita Lobo Balia
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pichia</i> β-glucan antifungal aflatoxin reducer Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/cbe2fb84d84b4525bb845e20e3492880
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spelling	oai:doaj.org-article:cbe2fb84d84b4525bb845e20e34928802021-11-25T17:33:30ZAntifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from <i>Pichia norvegensis</i> Grown on Tofu Wastewater10.3390/foods101126192304-8158https://doaj.org/article/cbe2fb84d84b4525bb845e20e34928802021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2619https://doaj.org/toc/2304-8158Yeast can be isolated from tofu wastewater and the cell wall in the form of β-glucan can act as a natural decontaminant agent. This study aimed to isolate and characterize native yeast from tofu wastewater, which can be extracted to obtain β-glucan and then identify the yeast and its β-glucan activity regarding antifungal ability against <i>Aspergillus flavus</i> and aflatoxin-reducing activity towards aflatoxin B1 (AFB1) and B2 (AFB2). Tofu wastewater native yeast was molecularly identified, and the growth observed based on optical density for 96 h and the pH also measured. β-glucan was extracted from native yeast cell walls with the acid-base method and then the inhibition activity towards <i>A. flavus</i> was tested using the well diffusion method and microscopic observation. AFB1 and AFB2 reduction were identified using HPLC LC-MS/MS. The results showed that the native yeast isolated was <i>Pichia norvegensis</i> with a β-glucan yield of 6.59%. <i>Pichia norvegensis</i> and its β-glucan showed an inhibition zone against <i>Aspergillus flavus</i> of 11.33 ± 4.93 and 7.33 ± 3.51 mm, respectively. Total aflatoxin-reducing activity was also shown by <i>Pichia norvegensis</i> of 26.85 ± 2.87%, and β-glucan of 27.30 ± 1.49%, while AFB1- and AFB2-reducing activity by <i>Pichia norvegensis</i> was 36.97 ± 3.07% and 27.13 ± 1.69%, and β-glucan was 27.13 ± 1.69% and 32.59 ± 4.20%, respectively.Gemilang Lara UtamaMahardhika Puspa Arum SuralokaTita RialitaRoostita Lobo BaliaMDPI AGarticle<i>Pichia</i>β-glucanantifungalaflatoxin reducerChemical technologyTP1-1185ENFoods, Vol 10, Iss 2619, p 2619 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pichia</i> β-glucan antifungal aflatoxin reducer Chemical technology TP1-1185
spellingShingle	<i>Pichia</i> β-glucan antifungal aflatoxin reducer Chemical technology TP1-1185 Gemilang Lara Utama Mahardhika Puspa Arum Suraloka Tita Rialita Roostita Lobo Balia Antifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from <i>Pichia norvegensis</i> Grown on Tofu Wastewater
description	Yeast can be isolated from tofu wastewater and the cell wall in the form of β-glucan can act as a natural decontaminant agent. This study aimed to isolate and characterize native yeast from tofu wastewater, which can be extracted to obtain β-glucan and then identify the yeast and its β-glucan activity regarding antifungal ability against <i>Aspergillus flavus</i> and aflatoxin-reducing activity towards aflatoxin B1 (AFB1) and B2 (AFB2). Tofu wastewater native yeast was molecularly identified, and the growth observed based on optical density for 96 h and the pH also measured. β-glucan was extracted from native yeast cell walls with the acid-base method and then the inhibition activity towards <i>A. flavus</i> was tested using the well diffusion method and microscopic observation. AFB1 and AFB2 reduction were identified using HPLC LC-MS/MS. The results showed that the native yeast isolated was <i>Pichia norvegensis</i> with a β-glucan yield of 6.59%. <i>Pichia norvegensis</i> and its β-glucan showed an inhibition zone against <i>Aspergillus flavus</i> of 11.33 ± 4.93 and 7.33 ± 3.51 mm, respectively. Total aflatoxin-reducing activity was also shown by <i>Pichia norvegensis</i> of 26.85 ± 2.87%, and β-glucan of 27.30 ± 1.49%, while AFB1- and AFB2-reducing activity by <i>Pichia norvegensis</i> was 36.97 ± 3.07% and 27.13 ± 1.69%, and β-glucan was 27.13 ± 1.69% and 32.59 ± 4.20%, respectively.
format	article
author	Gemilang Lara Utama Mahardhika Puspa Arum Suraloka Tita Rialita Roostita Lobo Balia
author_facet	Gemilang Lara Utama Mahardhika Puspa Arum Suraloka Tita Rialita Roostita Lobo Balia
author_sort	Gemilang Lara Utama
title	Antifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from <i>Pichia norvegensis</i> Grown on Tofu Wastewater
title_short	Antifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from <i>Pichia norvegensis</i> Grown on Tofu Wastewater
title_full	Antifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from <i>Pichia norvegensis</i> Grown on Tofu Wastewater
title_fullStr	Antifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from <i>Pichia norvegensis</i> Grown on Tofu Wastewater
title_full_unstemmed	Antifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from <i>Pichia norvegensis</i> Grown on Tofu Wastewater
title_sort	antifungal and aflatoxin-reducing activity of β-glucan isolated from <i>pichia norvegensis</i> grown on tofu wastewater
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cbe2fb84d84b4525bb845e20e3492880
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Antifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from <i>Pichia norvegensis</i> Grown on Tofu Wastewater

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