Control of axillary bud growth in tobacco through toxin gene expression system

Abstract The control of axillary bud development after removing the terminal buds (topping) of plants is a research hotspot, and the control of gene expression, like switching on and off, allows us to further study biological traits of interest, such as plant branching and fertility. In this study,...

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Autores principales:	Jing Lv, Ya-Qiong Chen, An-Ming Ding, Bo Lei, Jing Yu, Xiao-Ming Gao, Chang-Bo Dai, Yu-He Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cbf08862ac2a463a9dfcb3d3dcb6358c
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spelling	oai:doaj.org-article:cbf08862ac2a463a9dfcb3d3dcb6358c2021-12-02T17:51:12ZControl of axillary bud growth in tobacco through toxin gene expression system10.1038/s41598-021-96976-32045-2322https://doaj.org/article/cbf08862ac2a463a9dfcb3d3dcb6358c2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96976-3https://doaj.org/toc/2045-2322Abstract The control of axillary bud development after removing the terminal buds (topping) of plants is a research hotspot, and the control of gene expression, like switching on and off, allows us to further study biological traits of interest, such as plant branching and fertility. In this study, a toxin gene control system for plants based on dexamethasone (DEX) induction was constructed, and the positive transgenic tobacco exhibited growth retardation in the application area (axillary bud). The expression level of the lethal Diphtheria toxin A (DTA) gene under different DEX concentrations at different application days was analyzed. The highest expression levels appeared at 5 days after the leaf injection of DEX. The DTA transcripts were induced by 5 µM DEX and peaked in response to 50 µM DEX at 5 days after leaf injection. Here, a chemical induction system, combined with a toxin gene, were used to successfully control the growth of tobacco axillary buds after topping. The DTA expression system under DEX induction was sensitive and efficient, therefore, can be used to control axillary bud growth and development in tobacco.Jing LvYa-Qiong ChenAn-Ming DingBo LeiJing YuXiao-Ming GaoChang-Bo DaiYu-He SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Lv Ya-Qiong Chen An-Ming Ding Bo Lei Jing Yu Xiao-Ming Gao Chang-Bo Dai Yu-He Sun Control of axillary bud growth in tobacco through toxin gene expression system
description	Abstract The control of axillary bud development after removing the terminal buds (topping) of plants is a research hotspot, and the control of gene expression, like switching on and off, allows us to further study biological traits of interest, such as plant branching and fertility. In this study, a toxin gene control system for plants based on dexamethasone (DEX) induction was constructed, and the positive transgenic tobacco exhibited growth retardation in the application area (axillary bud). The expression level of the lethal Diphtheria toxin A (DTA) gene under different DEX concentrations at different application days was analyzed. The highest expression levels appeared at 5 days after the leaf injection of DEX. The DTA transcripts were induced by 5 µM DEX and peaked in response to 50 µM DEX at 5 days after leaf injection. Here, a chemical induction system, combined with a toxin gene, were used to successfully control the growth of tobacco axillary buds after topping. The DTA expression system under DEX induction was sensitive and efficient, therefore, can be used to control axillary bud growth and development in tobacco.
format	article
author	Jing Lv Ya-Qiong Chen An-Ming Ding Bo Lei Jing Yu Xiao-Ming Gao Chang-Bo Dai Yu-He Sun
author_facet	Jing Lv Ya-Qiong Chen An-Ming Ding Bo Lei Jing Yu Xiao-Ming Gao Chang-Bo Dai Yu-He Sun
author_sort	Jing Lv
title	Control of axillary bud growth in tobacco through toxin gene expression system
title_short	Control of axillary bud growth in tobacco through toxin gene expression system
title_full	Control of axillary bud growth in tobacco through toxin gene expression system
title_fullStr	Control of axillary bud growth in tobacco through toxin gene expression system
title_full_unstemmed	Control of axillary bud growth in tobacco through toxin gene expression system
title_sort	control of axillary bud growth in tobacco through toxin gene expression system
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cbf08862ac2a463a9dfcb3d3dcb6358c
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Control of axillary bud growth in tobacco through toxin gene expression system

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