Hypoxia imaging in cells and tumor tissues using a highly selective fluorescent nitroreductase probe

Abstract Hypoxia is a characteristic of locally advanced solid tumors, resulting from an imbalance between oxygen consumption and supply. In hypoxic solid tumors, an increased expression of nitroreductase (NTR) is detected, therefore, the development of NTR-targeted fluorescent probes to selectively...

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Autores principales:	Dan Yang, Hang Yu Tian, Tie Nan Zang, Ming Li, Ying Zhou, Jun Feng Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cbf1821b71684008af08a97dbb458eea
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spelling	oai:doaj.org-article:cbf1821b71684008af08a97dbb458eea2021-12-02T15:06:27ZHypoxia imaging in cells and tumor tissues using a highly selective fluorescent nitroreductase probe10.1038/s41598-017-09525-22045-2322https://doaj.org/article/cbf1821b71684008af08a97dbb458eea2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09525-2https://doaj.org/toc/2045-2322Abstract Hypoxia is a characteristic of locally advanced solid tumors, resulting from an imbalance between oxygen consumption and supply. In hypoxic solid tumors, an increased expression of nitroreductase (NTR) is detected, therefore, the development of NTR-targeted fluorescent probes to selectively and efficiently detect hypoxia in vivo is of utmost importance. In this study, a probe (1) has been designed and tested for effective optical detection of NTR in vitro and in vivo. The reduction of probe (1), catalyzed by NTR, resulted in changes of the electron-withdrawn nitrogen group into an electron-donation amino group. In addition, breakage of the O-C bond ensured selective fluorescence enhancement. The in vitro response towards exogenous NTR, from rat liver microsomes, resulted in the optical enhancement during the detection process. In vivo imaging of caerorhabditis elegans (C.elegan) further confirmed the detection of NTR by probe (1). Moreover, probe (1) was successfully used for the detection of hypoxia in both HI5 cells, and a murine tumor model, which demonstrates the potential of probe (1) for application in fluorescence bioimaging studies, and tumor hypoxia diagnosis.Dan YangHang Yu TianTie Nan ZangMing LiYing ZhouJun Feng ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dan Yang Hang Yu Tian Tie Nan Zang Ming Li Ying Zhou Jun Feng Zhang Hypoxia imaging in cells and tumor tissues using a highly selective fluorescent nitroreductase probe
description	Abstract Hypoxia is a characteristic of locally advanced solid tumors, resulting from an imbalance between oxygen consumption and supply. In hypoxic solid tumors, an increased expression of nitroreductase (NTR) is detected, therefore, the development of NTR-targeted fluorescent probes to selectively and efficiently detect hypoxia in vivo is of utmost importance. In this study, a probe (1) has been designed and tested for effective optical detection of NTR in vitro and in vivo. The reduction of probe (1), catalyzed by NTR, resulted in changes of the electron-withdrawn nitrogen group into an electron-donation amino group. In addition, breakage of the O-C bond ensured selective fluorescence enhancement. The in vitro response towards exogenous NTR, from rat liver microsomes, resulted in the optical enhancement during the detection process. In vivo imaging of caerorhabditis elegans (C.elegan) further confirmed the detection of NTR by probe (1). Moreover, probe (1) was successfully used for the detection of hypoxia in both HI5 cells, and a murine tumor model, which demonstrates the potential of probe (1) for application in fluorescence bioimaging studies, and tumor hypoxia diagnosis.
format	article
author	Dan Yang Hang Yu Tian Tie Nan Zang Ming Li Ying Zhou Jun Feng Zhang
author_facet	Dan Yang Hang Yu Tian Tie Nan Zang Ming Li Ying Zhou Jun Feng Zhang
author_sort	Dan Yang
title	Hypoxia imaging in cells and tumor tissues using a highly selective fluorescent nitroreductase probe
title_short	Hypoxia imaging in cells and tumor tissues using a highly selective fluorescent nitroreductase probe
title_full	Hypoxia imaging in cells and tumor tissues using a highly selective fluorescent nitroreductase probe
title_fullStr	Hypoxia imaging in cells and tumor tissues using a highly selective fluorescent nitroreductase probe
title_full_unstemmed	Hypoxia imaging in cells and tumor tissues using a highly selective fluorescent nitroreductase probe
title_sort	hypoxia imaging in cells and tumor tissues using a highly selective fluorescent nitroreductase probe
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cbf1821b71684008af08a97dbb458eea
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_version_	1718388479061131264

Hypoxia imaging in cells and tumor tissues using a highly selective fluorescent nitroreductase probe

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