Chemokine-like Receptor 1 in Brain of Spontaneously Hypertensive Rats Mediates Systemic Hypertension

Adipocytokine chemerin is a biologically active molecule secreted from adipose tissue. Chemerin elicits a variety of functions via chemokine-like receptor 1 (CMKLR1). The cardiovascular center in brain that regulates blood pressure (BP) is involved in pathophysiology of systemic hypertension. Thus,...

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Autores principales:	Atsunori Yamamoto, Kosuke Otani, Muneyoshi Okada, Hideyuki Yamawaki
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adipocytokine chemerin CMKLR1 PVN hypertension Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/cbf23053e9694d2faa35d98454f038c6
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spelling	oai:doaj.org-article:cbf23053e9694d2faa35d98454f038c62021-11-11T17:15:13ZChemokine-like Receptor 1 in Brain of Spontaneously Hypertensive Rats Mediates Systemic Hypertension10.3390/ijms2221118121422-00671661-6596https://doaj.org/article/cbf23053e9694d2faa35d98454f038c62021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11812https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Adipocytokine chemerin is a biologically active molecule secreted from adipose tissue. Chemerin elicits a variety of functions via chemokine-like receptor 1 (CMKLR1). The cardiovascular center in brain that regulates blood pressure (BP) is involved in pathophysiology of systemic hypertension. Thus, we explored the roles of brain chemerin/CMKLR1 on regulation of BP in spontaneously hypertensive rats (SHR). For this aim, we examined effects of intracerebroventricular (i.c.v.) injection of CMKLR1 small interfering (si)RNA on both systemic BP as measured by tail cuff system and protein expression in paraventricular nucleus (PVN) of SHR as determined by Western blotting. We also examined both central and peripheral protein expression of chemerin by Western blotting. Systolic BP of SHR but not normotensive Wistar Kyoto rats (WKY) was decreased by CMKLR1 siRNA. The decrease of BP by CMKLR1 siRNA persisted for 3 days. Protein expression of CMKLR1 in PVN of SHR tended to be increased compared with WKY, which was suppressed by CMKLR1 siRNA. Protein expression of chemerin in brain, peripheral plasma, and adipose tissue was not different between WKY and SHR. In summary, we for the first time revealed that the increased protein expression of CMKLR1 in PVN is at least partly responsible for systemic hypertension in SHR.Atsunori YamamotoKosuke OtaniMuneyoshi OkadaHideyuki YamawakiMDPI AGarticleadipocytokinechemerinCMKLR1PVNhypertensionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11812, p 11812 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipocytokine chemerin CMKLR1 PVN hypertension Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	adipocytokine chemerin CMKLR1 PVN hypertension Biology (General) QH301-705.5 Chemistry QD1-999 Atsunori Yamamoto Kosuke Otani Muneyoshi Okada Hideyuki Yamawaki Chemokine-like Receptor 1 in Brain of Spontaneously Hypertensive Rats Mediates Systemic Hypertension
description	Adipocytokine chemerin is a biologically active molecule secreted from adipose tissue. Chemerin elicits a variety of functions via chemokine-like receptor 1 (CMKLR1). The cardiovascular center in brain that regulates blood pressure (BP) is involved in pathophysiology of systemic hypertension. Thus, we explored the roles of brain chemerin/CMKLR1 on regulation of BP in spontaneously hypertensive rats (SHR). For this aim, we examined effects of intracerebroventricular (i.c.v.) injection of CMKLR1 small interfering (si)RNA on both systemic BP as measured by tail cuff system and protein expression in paraventricular nucleus (PVN) of SHR as determined by Western blotting. We also examined both central and peripheral protein expression of chemerin by Western blotting. Systolic BP of SHR but not normotensive Wistar Kyoto rats (WKY) was decreased by CMKLR1 siRNA. The decrease of BP by CMKLR1 siRNA persisted for 3 days. Protein expression of CMKLR1 in PVN of SHR tended to be increased compared with WKY, which was suppressed by CMKLR1 siRNA. Protein expression of chemerin in brain, peripheral plasma, and adipose tissue was not different between WKY and SHR. In summary, we for the first time revealed that the increased protein expression of CMKLR1 in PVN is at least partly responsible for systemic hypertension in SHR.
format	article
author	Atsunori Yamamoto Kosuke Otani Muneyoshi Okada Hideyuki Yamawaki
author_facet	Atsunori Yamamoto Kosuke Otani Muneyoshi Okada Hideyuki Yamawaki
author_sort	Atsunori Yamamoto
title	Chemokine-like Receptor 1 in Brain of Spontaneously Hypertensive Rats Mediates Systemic Hypertension
title_short	Chemokine-like Receptor 1 in Brain of Spontaneously Hypertensive Rats Mediates Systemic Hypertension
title_full	Chemokine-like Receptor 1 in Brain of Spontaneously Hypertensive Rats Mediates Systemic Hypertension
title_fullStr	Chemokine-like Receptor 1 in Brain of Spontaneously Hypertensive Rats Mediates Systemic Hypertension
title_full_unstemmed	Chemokine-like Receptor 1 in Brain of Spontaneously Hypertensive Rats Mediates Systemic Hypertension
title_sort	chemokine-like receptor 1 in brain of spontaneously hypertensive rats mediates systemic hypertension
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cbf23053e9694d2faa35d98454f038c6
work_keys_str_mv	AT atsunoriyamamoto chemokinelikereceptor1inbrainofspontaneouslyhypertensiveratsmediatessystemichypertension AT kosukeotani chemokinelikereceptor1inbrainofspontaneouslyhypertensiveratsmediatessystemichypertension AT muneyoshiokada chemokinelikereceptor1inbrainofspontaneouslyhypertensiveratsmediatessystemichypertension AT hideyukiyamawaki chemokinelikereceptor1inbrainofspontaneouslyhypertensiveratsmediatessystemichypertension
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Chemokine-like Receptor 1 in Brain of Spontaneously Hypertensive Rats Mediates Systemic Hypertension

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