An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in <i>Lycoris aurea</i>

As a kind of Amaryllidaceae alkaloid which is accumulated in the species of Lycoris plants, lycorine has a range of physiological effects. The biosynthesis pathway of lycorine has been partly revealed, but the transport and accumulation mechanisms of lycorine have rarely been studied. In this study,...

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Autores principales:	Rong Wang, Yantong Liu, Sheng Xu, Jie Li, Jiayu Zhou, Ren Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ABC transporter lycorine galantamine <i>Lycoris aurea</i> Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/cbf365fc7d56438fae4fab06e1aaf251
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spelling	oai:doaj.org-article:cbf365fc7d56438fae4fab06e1aaf2512021-11-11T16:55:19ZAn ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in <i>Lycoris aurea</i>10.3390/ijms2221114581422-00671661-6596https://doaj.org/article/cbf365fc7d56438fae4fab06e1aaf2512021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11458https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067As a kind of Amaryllidaceae alkaloid which is accumulated in the species of Lycoris plants, lycorine has a range of physiological effects. The biosynthesis pathway of lycorine has been partly revealed, but the transport and accumulation mechanisms of lycorine have rarely been studied. In this study, an ATP-binding cassette (ABC) transporter from <i>Lycoris aurea</i> (L’Hér) Herb., namely LaABCB11, was cloned and functionally characterized. Heterologous expression showed that LaABCB11 transported lycorine in an outward direction, increased the tolerance of yeast cells to lycorine, and caused a lower lycorine accumulation in transformants than control or mutant in yeast. LaABCB11 is associated with the plasma membrane, and in situ hybridization indicated that <i>LaABCB11</i> was mainly expressed in the phloem of leaves and bulbs, as well as in the cortical cells of roots. These findings suggest that LaABCB11 functions as a lycorine transport and it might be related to the translocation and accumulation of lycorine from the leaves and bulbs to the roots.Rong WangYantong LiuSheng XuJie LiJiayu ZhouRen WangMDPI AGarticleABC transporterlycorinegalantamine<i>Lycoris aurea</i>Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11458, p 11458 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ABC transporter lycorine galantamine <i>Lycoris aurea</i> Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	ABC transporter lycorine galantamine <i>Lycoris aurea</i> Biology (General) QH301-705.5 Chemistry QD1-999 Rong Wang Yantong Liu Sheng Xu Jie Li Jiayu Zhou Ren Wang An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in <i>Lycoris aurea</i>
description	As a kind of Amaryllidaceae alkaloid which is accumulated in the species of Lycoris plants, lycorine has a range of physiological effects. The biosynthesis pathway of lycorine has been partly revealed, but the transport and accumulation mechanisms of lycorine have rarely been studied. In this study, an ATP-binding cassette (ABC) transporter from <i>Lycoris aurea</i> (L’Hér) Herb., namely LaABCB11, was cloned and functionally characterized. Heterologous expression showed that LaABCB11 transported lycorine in an outward direction, increased the tolerance of yeast cells to lycorine, and caused a lower lycorine accumulation in transformants than control or mutant in yeast. LaABCB11 is associated with the plasma membrane, and in situ hybridization indicated that <i>LaABCB11</i> was mainly expressed in the phloem of leaves and bulbs, as well as in the cortical cells of roots. These findings suggest that LaABCB11 functions as a lycorine transport and it might be related to the translocation and accumulation of lycorine from the leaves and bulbs to the roots.
format	article
author	Rong Wang Yantong Liu Sheng Xu Jie Li Jiayu Zhou Ren Wang
author_facet	Rong Wang Yantong Liu Sheng Xu Jie Li Jiayu Zhou Ren Wang
author_sort	Rong Wang
title	An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in <i>Lycoris aurea</i>
title_short	An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in <i>Lycoris aurea</i>
title_full	An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in <i>Lycoris aurea</i>
title_fullStr	An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in <i>Lycoris aurea</i>
title_full_unstemmed	An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in <i>Lycoris aurea</i>
title_sort	atp-binding cassette transporter, laabcb11, contributes to alkaloid transport in <i>lycoris aurea</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cbf365fc7d56438fae4fab06e1aaf251
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An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in <i>Lycoris aurea</i>

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