The endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (SW 872).

Esters of phthalic acid (phthalates) are largely used in industrial plastics, medical devices, and pharmaceutical formulations. They are easily released from plastics into the environment and can be found in measurable levels in human fluids. Phthalates are agonists for peroxisome proliferator-activ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Enrico Campioli, Amani Batarseh, Jiehan Li, Vassilios Papadopoulos
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cbf555d460d84c1688f6ecb0bb2b5913
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cbf555d460d84c1688f6ecb0bb2b5913
record_format	dspace
spelling	oai:doaj.org-article:cbf555d460d84c1688f6ecb0bb2b59132021-11-18T07:31:45ZThe endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (SW 872).1932-620310.1371/journal.pone.0028750https://doaj.org/article/cbf555d460d84c1688f6ecb0bb2b59132011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22205965/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Esters of phthalic acid (phthalates) are largely used in industrial plastics, medical devices, and pharmaceutical formulations. They are easily released from plastics into the environment and can be found in measurable levels in human fluids. Phthalates are agonists for peroxisome proliferator-activated receptors (PPARs), through which they regulate translocator protein (TSPO; 18 kDa) transcription in a tissue-specific manner. TSPO is a drug- and cholesterol-binding protein involved in mitochondrial respiration, steroid formation, and cell proliferation. TSPO has been shown to increase during differentiation and decrease during maturation in mouse adipocytes. The purpose of this study was to establish the effect of mono-(2-ethylhexyl) phthalate (MEHP) on the differentiation of human SW 872 preadipocyte cells, and examine the role of TSPO in the process. After 4 days of treatment with 10 µM MEHP, we observed changes in the transcription of acetyl-CoA carboxylase alpha, adenosine triphosphate citrate lyase, glucose transporters 1 and 4, and the S100 calcium binding protein B, all of which are markers of preadipocyte differentiation. These observed gene expression changes coincided with a decrease in cellular proliferation without affecting cellular triglyceride content. Taken together, these data suggest that MEHP exerts a differentiating effect on human preadipocytes. Interestingly, MEHP was able to temporarily increase TSPO mRNA levels through the PPAR-α and β/δ pathways. These results suggest that TSPO can be considered an important player in the differentiation process itself, or alternatively a factor whose presence is essential for adipocyte development.Enrico CampioliAmani BatarsehJiehan LiVassilios PapadopoulosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28750 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Enrico Campioli Amani Batarseh Jiehan Li Vassilios Papadopoulos The endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (SW 872).
description	Esters of phthalic acid (phthalates) are largely used in industrial plastics, medical devices, and pharmaceutical formulations. They are easily released from plastics into the environment and can be found in measurable levels in human fluids. Phthalates are agonists for peroxisome proliferator-activated receptors (PPARs), through which they regulate translocator protein (TSPO; 18 kDa) transcription in a tissue-specific manner. TSPO is a drug- and cholesterol-binding protein involved in mitochondrial respiration, steroid formation, and cell proliferation. TSPO has been shown to increase during differentiation and decrease during maturation in mouse adipocytes. The purpose of this study was to establish the effect of mono-(2-ethylhexyl) phthalate (MEHP) on the differentiation of human SW 872 preadipocyte cells, and examine the role of TSPO in the process. After 4 days of treatment with 10 µM MEHP, we observed changes in the transcription of acetyl-CoA carboxylase alpha, adenosine triphosphate citrate lyase, glucose transporters 1 and 4, and the S100 calcium binding protein B, all of which are markers of preadipocyte differentiation. These observed gene expression changes coincided with a decrease in cellular proliferation without affecting cellular triglyceride content. Taken together, these data suggest that MEHP exerts a differentiating effect on human preadipocytes. Interestingly, MEHP was able to temporarily increase TSPO mRNA levels through the PPAR-α and β/δ pathways. These results suggest that TSPO can be considered an important player in the differentiation process itself, or alternatively a factor whose presence is essential for adipocyte development.
format	article
author	Enrico Campioli Amani Batarseh Jiehan Li Vassilios Papadopoulos
author_facet	Enrico Campioli Amani Batarseh Jiehan Li Vassilios Papadopoulos
author_sort	Enrico Campioli
title	The endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (SW 872).
title_short	The endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (SW 872).
title_full	The endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (SW 872).
title_fullStr	The endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (SW 872).
title_full_unstemmed	The endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (SW 872).
title_sort	endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (sw 872).
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/cbf555d460d84c1688f6ecb0bb2b5913
work_keys_str_mv	AT enricocampioli theendocrinedisruptormono2ethylhexylphthalateaffectsthedifferentiationofhumanliposarcomacellssw872 AT amanibatarseh theendocrinedisruptormono2ethylhexylphthalateaffectsthedifferentiationofhumanliposarcomacellssw872 AT jiehanli theendocrinedisruptormono2ethylhexylphthalateaffectsthedifferentiationofhumanliposarcomacellssw872 AT vassiliospapadopoulos theendocrinedisruptormono2ethylhexylphthalateaffectsthedifferentiationofhumanliposarcomacellssw872 AT enricocampioli endocrinedisruptormono2ethylhexylphthalateaffectsthedifferentiationofhumanliposarcomacellssw872 AT amanibatarseh endocrinedisruptormono2ethylhexylphthalateaffectsthedifferentiationofhumanliposarcomacellssw872 AT jiehanli endocrinedisruptormono2ethylhexylphthalateaffectsthedifferentiationofhumanliposarcomacellssw872 AT vassiliospapadopoulos endocrinedisruptormono2ethylhexylphthalateaffectsthedifferentiationofhumanliposarcomacellssw872
_version_	1718423314166185984

The endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (SW 872).

Ejemplares similares