Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W

UBE2W catalyzes the ubiquitination of protein N-termini but its substrate spectrum is largely unknown. Here, the authors discover mAbs selective for peptides derived from N-terminally ubiquitinated proteins, solve the structure of a peptide-bound mAb and apply the mAbs to map endogenous UBE2W substr...

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Autores principales:	Christopher W. Davies, Simon E. Vidal, Lilian Phu, Jawahar Sudhamsu, Trent B. Hinkle, Scott Chan Rosenberg, Frances-Rose Schumacher, Yi Jimmy Zeng, Carsten Schwerdtfeger, Andrew S. Peterson, Jennie R. Lill, Christopher M. Rose, Andrey S. Shaw, Ingrid E. Wertz, Donald S. Kirkpatrick, James T. Koerber
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/cbfb2674260f4b51a1c495c83e07dcb5
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spelling	oai:doaj.org-article:cbfb2674260f4b51a1c495c83e07dcb52021-12-02T16:31:49ZAntibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W10.1038/s41467-021-24669-62041-1723https://doaj.org/article/cbfb2674260f4b51a1c495c83e07dcb52021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24669-6https://doaj.org/toc/2041-1723UBE2W catalyzes the ubiquitination of protein N-termini but its substrate spectrum is largely unknown. Here, the authors discover mAbs selective for peptides derived from N-terminally ubiquitinated proteins, solve the structure of a peptide-bound mAb and apply the mAbs to map endogenous UBE2W substrates by proteomics.Christopher W. DaviesSimon E. VidalLilian PhuJawahar SudhamsuTrent B. HinkleScott Chan RosenbergFrances-Rose SchumacherYi Jimmy ZengCarsten SchwerdtfegerAndrew S. PetersonJennie R. LillChristopher M. RoseAndrey S. ShawIngrid E. WertzDonald S. KirkpatrickJames T. KoerberNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Christopher W. Davies Simon E. Vidal Lilian Phu Jawahar Sudhamsu Trent B. Hinkle Scott Chan Rosenberg Frances-Rose Schumacher Yi Jimmy Zeng Carsten Schwerdtfeger Andrew S. Peterson Jennie R. Lill Christopher M. Rose Andrey S. Shaw Ingrid E. Wertz Donald S. Kirkpatrick James T. Koerber Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
description	UBE2W catalyzes the ubiquitination of protein N-termini but its substrate spectrum is largely unknown. Here, the authors discover mAbs selective for peptides derived from N-terminally ubiquitinated proteins, solve the structure of a peptide-bound mAb and apply the mAbs to map endogenous UBE2W substrates by proteomics.
format	article
author	Christopher W. Davies Simon E. Vidal Lilian Phu Jawahar Sudhamsu Trent B. Hinkle Scott Chan Rosenberg Frances-Rose Schumacher Yi Jimmy Zeng Carsten Schwerdtfeger Andrew S. Peterson Jennie R. Lill Christopher M. Rose Andrey S. Shaw Ingrid E. Wertz Donald S. Kirkpatrick James T. Koerber
author_facet	Christopher W. Davies Simon E. Vidal Lilian Phu Jawahar Sudhamsu Trent B. Hinkle Scott Chan Rosenberg Frances-Rose Schumacher Yi Jimmy Zeng Carsten Schwerdtfeger Andrew S. Peterson Jennie R. Lill Christopher M. Rose Andrey S. Shaw Ingrid E. Wertz Donald S. Kirkpatrick James T. Koerber
author_sort	Christopher W. Davies
title	Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
title_short	Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
title_full	Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
title_fullStr	Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
title_full_unstemmed	Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
title_sort	antibody toolkit reveals n-terminally ubiquitinated substrates of ube2w
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cbfb2674260f4b51a1c495c83e07dcb5
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Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W

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