Exosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis

Abstract In order to explore the role of exosomal circRNAs in the occurrence and development of sepsis, we looked for potential diagnostic markers to accurately identify sepsis and to lay a molecular basis for precise treatment. Ultracentrifugation was used to extract exosomes from the serum of pati...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chang Tian, Jiaying Liu, Xin Di, Shan Cong, Min Zhao, Ke Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cc02260f2eb1435083a6e3747fe47271
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cc02260f2eb1435083a6e3747fe47271
record_format	dspace
spelling	oai:doaj.org-article:cc02260f2eb1435083a6e3747fe472712021-12-02T16:14:46ZExosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis10.1038/s41598-021-93246-02045-2322https://doaj.org/article/cc02260f2eb1435083a6e3747fe472712021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93246-0https://doaj.org/toc/2045-2322Abstract In order to explore the role of exosomal circRNAs in the occurrence and development of sepsis, we looked for potential diagnostic markers to accurately identify sepsis and to lay a molecular basis for precise treatment. Ultracentrifugation was used to extract exosomes from the serum of patients with sepsis and healthy individuals. Then, changes in circRNA expression in exosomes were studied by circRNA microarray analysis. Gene ontology (GO) analysis and Kyoto City Encyclopaedia of Genes and Genomes (KEGG) pathway analysis were used to annotate the biological functions and pathways of genes, and a circRNA-miRNA-mRNA regulatory network was constructed. In the microarray analysis, 132 circRNAs were significantly differentially expressed, including 80 and 52 that were upregulated and downregulated, respectively. RT-qPCR verified the results of microarray analysis: hsa_circRNA_104484 and hsa_circRNA_104670 were upregulated in sepsis serum exosomes. ROC analysis showed that hsa_circRNA_104484 and hsa_circRNA_104670 in serum exosomes have the potential to be used as diagnostic markers for sepsis. The circRNA-miRNA-mRNA network predicted the potential regulatory pathways of differentially expressed circRNAs. There are differences in the expression of circRNA in serum exosomes between patients with sepsis and healthy individuals, which may be involved in the occurrence and development of the disease. Among them, elevations in hsa_circRNA_104484 and hsa_circRNA_104670 could be used as novel diagnostic biomarkers and molecular therapeutic targets.Chang TianJiaying LiuXin DiShan CongMin ZhaoKe WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chang Tian Jiaying Liu Xin Di Shan Cong Min Zhao Ke Wang Exosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis
description	Abstract In order to explore the role of exosomal circRNAs in the occurrence and development of sepsis, we looked for potential diagnostic markers to accurately identify sepsis and to lay a molecular basis for precise treatment. Ultracentrifugation was used to extract exosomes from the serum of patients with sepsis and healthy individuals. Then, changes in circRNA expression in exosomes were studied by circRNA microarray analysis. Gene ontology (GO) analysis and Kyoto City Encyclopaedia of Genes and Genomes (KEGG) pathway analysis were used to annotate the biological functions and pathways of genes, and a circRNA-miRNA-mRNA regulatory network was constructed. In the microarray analysis, 132 circRNAs were significantly differentially expressed, including 80 and 52 that were upregulated and downregulated, respectively. RT-qPCR verified the results of microarray analysis: hsa_circRNA_104484 and hsa_circRNA_104670 were upregulated in sepsis serum exosomes. ROC analysis showed that hsa_circRNA_104484 and hsa_circRNA_104670 in serum exosomes have the potential to be used as diagnostic markers for sepsis. The circRNA-miRNA-mRNA network predicted the potential regulatory pathways of differentially expressed circRNAs. There are differences in the expression of circRNA in serum exosomes between patients with sepsis and healthy individuals, which may be involved in the occurrence and development of the disease. Among them, elevations in hsa_circRNA_104484 and hsa_circRNA_104670 could be used as novel diagnostic biomarkers and molecular therapeutic targets.
format	article
author	Chang Tian Jiaying Liu Xin Di Shan Cong Min Zhao Ke Wang
author_facet	Chang Tian Jiaying Liu Xin Di Shan Cong Min Zhao Ke Wang
author_sort	Chang Tian
title	Exosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis
title_short	Exosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis
title_full	Exosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis
title_fullStr	Exosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis
title_full_unstemmed	Exosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis
title_sort	exosomal hsa_circrna_104484 and hsa_circrna_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cc02260f2eb1435083a6e3747fe47271
work_keys_str_mv	AT changtian exosomalhsacircrna104484andhsacircrna104670mayserveaspotentialnovelbiomarkersandtherapeutictargetsforsepsis AT jiayingliu exosomalhsacircrna104484andhsacircrna104670mayserveaspotentialnovelbiomarkersandtherapeutictargetsforsepsis AT xindi exosomalhsacircrna104484andhsacircrna104670mayserveaspotentialnovelbiomarkersandtherapeutictargetsforsepsis AT shancong exosomalhsacircrna104484andhsacircrna104670mayserveaspotentialnovelbiomarkersandtherapeutictargetsforsepsis AT minzhao exosomalhsacircrna104484andhsacircrna104670mayserveaspotentialnovelbiomarkersandtherapeutictargetsforsepsis AT kewang exosomalhsacircrna104484andhsacircrna104670mayserveaspotentialnovelbiomarkersandtherapeutictargetsforsepsis
_version_	1718384318677516288

Exosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis

Ejemplares similares