A Simple Route to the Complexation of Lutein with Reduced Graphene Oxide Nanocarriers and Antioxidant Protection Against Blue Light

Seon Yeong Chae,1 Min Chan Shin,2 Sangheon Jeon,2 Moon Sung Kang,2 Dong-Wook Han,2 Suck Won Hong2 1Research Center for Dielectric and Advanced Matter Physics, Pusan National University, Busan, 46241, Republic of Korea; 2Department of Cogno-Mechatronics Engineering, Department of Optics and Mechatron...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chae SY, Shin MC, Jeon S, Kang MS, Han DW, Hong SW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	reduced graphene oxide lutein antioxidant blue light nanocarrier Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cc0328cfd21243be935983b9d56234da
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cc0328cfd21243be935983b9d56234da
record_format	dspace
spelling	oai:doaj.org-article:cc0328cfd21243be935983b9d56234da2021-12-02T18:07:11ZA Simple Route to the Complexation of Lutein with Reduced Graphene Oxide Nanocarriers and Antioxidant Protection Against Blue Light1178-2013https://doaj.org/article/cc0328cfd21243be935983b9d56234da2021-10-01T00:00:00Zhttps://www.dovepress.com/a-simple-route-to-the-complexation-of-lutein-with-reduced-graphene-oxi-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Seon Yeong Chae,1 Min Chan Shin,2 Sangheon Jeon,2 Moon Sung Kang,2 Dong-Wook Han,2 Suck Won Hong2 1Research Center for Dielectric and Advanced Matter Physics, Pusan National University, Busan, 46241, Republic of Korea; 2Department of Cogno-Mechatronics Engineering, Department of Optics and Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan, 46241, Republic of KoreaCorrespondence: Suck Won Hong Email swhong@pusan.ac.krBackground: The excellent physicochemical properties of graphene-based materials, including graphene oxide (GO) and reduced GO (rGO), offer significant technological potential as multifunctional nanomaterials in biomedical fields. Lutein is a type of carotenoid that forms human macular pigments in the retina, where it inhibits harmful blue light and contributes to the strengthening of the antioxidant defense of retinal pigment epithelium cells.Methods: Synthesis of the Lutein-rGO (Lu–rGO) complex was carried out for the optimized concentration. Then characterization of material was analyzed through ultraviolet-visible spectrophotometer (UV-Vis spectra), Fourier-transform infrared spectroscopy (FT-IR), Raman spectroscopy, x-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM). Antioxidant activity of Lu–rGO complex was measured by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2.2-diphenyl-1-picrylhydrazyl (DPPH), glutathione (GSH) oxidation assay. Then, oxidative stress induction by blue light and analyzed intracellular reactive oxygen species (ROS).Results and Conclusion: Based on the FT-IR measurement, the reduction efficiency defined by area was found to be 87.3%, the ID/IG ratio of 0.98 demonstrated by the Lu–rGO complex in the Raman spectrum was slightly higher than that of the original GO. The exhibited significant decrease in the peak intensities of the oxygen functional groups of the XPS spectra of the Lu–rGO complex was observed compared with the GO. In the TEM image for the Lu–rGO complex, folded and wrinkled nanostructures over the lutein-covered rGO surface were evidenced by tight molecular binding. The Lu–rGO complex provided superior DPPH and ABTS radical scavenging activity than GO and lutein alone, and the oxidation of GSH was suppressed. It was confirmed that the content of intracellular ROS and lysosomes, increased by blue light, was reduced after treatment with the Lu–rGO complex on ARPE-19 cells. In summary, graphene-based nanocarriers could function as preventative antioxidants during photochemical ROS generation based on the mechanism of antioxidant action.Keywords: reduced graphene oxide, lutein, antioxidant, blue light, nanocarrierChae SYShin MCJeon SKang MSHan DWHong SWDove Medical Pressarticlereduced graphene oxideluteinantioxidantblue lightnanocarrierMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 6843-6860 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	reduced graphene oxide lutein antioxidant blue light nanocarrier Medicine (General) R5-920
spellingShingle	reduced graphene oxide lutein antioxidant blue light nanocarrier Medicine (General) R5-920 Chae SY Shin MC Jeon S Kang MS Han DW Hong SW A Simple Route to the Complexation of Lutein with Reduced Graphene Oxide Nanocarriers and Antioxidant Protection Against Blue Light
description	Seon Yeong Chae,1 Min Chan Shin,2 Sangheon Jeon,2 Moon Sung Kang,2 Dong-Wook Han,2 Suck Won Hong2 1Research Center for Dielectric and Advanced Matter Physics, Pusan National University, Busan, 46241, Republic of Korea; 2Department of Cogno-Mechatronics Engineering, Department of Optics and Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan, 46241, Republic of KoreaCorrespondence: Suck Won Hong Email swhong@pusan.ac.krBackground: The excellent physicochemical properties of graphene-based materials, including graphene oxide (GO) and reduced GO (rGO), offer significant technological potential as multifunctional nanomaterials in biomedical fields. Lutein is a type of carotenoid that forms human macular pigments in the retina, where it inhibits harmful blue light and contributes to the strengthening of the antioxidant defense of retinal pigment epithelium cells.Methods: Synthesis of the Lutein-rGO (Lu–rGO) complex was carried out for the optimized concentration. Then characterization of material was analyzed through ultraviolet-visible spectrophotometer (UV-Vis spectra), Fourier-transform infrared spectroscopy (FT-IR), Raman spectroscopy, x-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM). Antioxidant activity of Lu–rGO complex was measured by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2.2-diphenyl-1-picrylhydrazyl (DPPH), glutathione (GSH) oxidation assay. Then, oxidative stress induction by blue light and analyzed intracellular reactive oxygen species (ROS).Results and Conclusion: Based on the FT-IR measurement, the reduction efficiency defined by area was found to be 87.3%, the ID/IG ratio of 0.98 demonstrated by the Lu–rGO complex in the Raman spectrum was slightly higher than that of the original GO. The exhibited significant decrease in the peak intensities of the oxygen functional groups of the XPS spectra of the Lu–rGO complex was observed compared with the GO. In the TEM image for the Lu–rGO complex, folded and wrinkled nanostructures over the lutein-covered rGO surface were evidenced by tight molecular binding. The Lu–rGO complex provided superior DPPH and ABTS radical scavenging activity than GO and lutein alone, and the oxidation of GSH was suppressed. It was confirmed that the content of intracellular ROS and lysosomes, increased by blue light, was reduced after treatment with the Lu–rGO complex on ARPE-19 cells. In summary, graphene-based nanocarriers could function as preventative antioxidants during photochemical ROS generation based on the mechanism of antioxidant action.Keywords: reduced graphene oxide, lutein, antioxidant, blue light, nanocarrier
format	article
author	Chae SY Shin MC Jeon S Kang MS Han DW Hong SW
author_facet	Chae SY Shin MC Jeon S Kang MS Han DW Hong SW
author_sort	Chae SY
title	A Simple Route to the Complexation of Lutein with Reduced Graphene Oxide Nanocarriers and Antioxidant Protection Against Blue Light
title_short	A Simple Route to the Complexation of Lutein with Reduced Graphene Oxide Nanocarriers and Antioxidant Protection Against Blue Light
title_full	A Simple Route to the Complexation of Lutein with Reduced Graphene Oxide Nanocarriers and Antioxidant Protection Against Blue Light
title_fullStr	A Simple Route to the Complexation of Lutein with Reduced Graphene Oxide Nanocarriers and Antioxidant Protection Against Blue Light
title_full_unstemmed	A Simple Route to the Complexation of Lutein with Reduced Graphene Oxide Nanocarriers and Antioxidant Protection Against Blue Light
title_sort	simple route to the complexation of lutein with reduced graphene oxide nanocarriers and antioxidant protection against blue light
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/cc0328cfd21243be935983b9d56234da
work_keys_str_mv	AT chaesy asimpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT shinmc asimpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT jeons asimpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT kangms asimpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT handw asimpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT hongsw asimpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT chaesy simpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT shinmc simpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT jeons simpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT kangms simpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT handw simpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight AT hongsw simpleroutetothecomplexationofluteinwithreducedgrapheneoxidenanocarriersandantioxidantprotectionagainstbluelight
_version_	1718378657641136128

A Simple Route to the Complexation of Lutein with Reduced Graphene Oxide Nanocarriers and Antioxidant Protection Against Blue Light

Ejemplares similares