Isolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks

Background: Foodborne diseases are a major problem worldwide. The epidemiological investigations in many parts of the world have shown an increase in infections caused by Salmonella serovars. Furthermore, the emergence of drug resistance among them has become a major global concern and awareness of...

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Autores principales:	Mohammad Mehdi Soltan Dallal, Milad Abdi, Mahya Khalilian, Zahra Rajabi, Ronak Bakhtiari, Mohammad Kazem Sharifi Yazdi, Somayeh Yaslianifard, Seyedeh Masoumeh Abrishamchian Langroudi
Formato:	article
Lenguaje:	EN
Publicado:	Alborz University of Medical Sciences 2020
Materias:	salmonella foodborne diseases outbreak diarrhea antibiotic susceptibility testing Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/cc05b0f5d64b4da68b30a8bcef98ba74
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spelling	oai:doaj.org-article:cc05b0f5d64b4da68b30a8bcef98ba742021-11-18T06:56:46ZIsolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks2345-33622322-586610.34172/ijep.2020.18https://doaj.org/article/cc05b0f5d64b4da68b30a8bcef98ba742020-08-01T00:00:00Zhttp://enterpathog.abzums.ac.ir/PDF/ijep-4342https://doaj.org/toc/2345-3362https://doaj.org/toc/2322-5866Background: Foodborne diseases are a major problem worldwide. The epidemiological investigations in many parts of the world have shown an increase in infections caused by Salmonella serovars. Furthermore, the emergence of drug resistance among them has become a major global concern and awareness of the resistance patterns of Salmonella could be very useful in treatment of diseases. Objective: This study aimed to investigate Salmonella serotypes in foodborne outbreaks by sequencing of ITS region of 16S-23SrRNA gene and to determine their antimicrobial susceptibility pattern. Materials and Methods: A total of 614 diarrheal stool samples were collected from 173 foodborne outbreaks in different provinces of Iran during one year. Identification of Salmonella was carried out by phenotypic and molecular (16s-23srRNA gene detection) methods and antibiotic susceptibility was performed using disc diffusion method. Results: Out of 614 samples, 18 isolates were identified as Salmonella of which 16 (88.9%) isolates were Salmonella Enteritidis and 2 (11.1%) isolates as Salmonella Paratyphi A. All isolates were sensitive to ceftazidime, and high resistance was seen with nalidixic acid with 14 (77.8%) isolates. Conclusion: Increasing antibiotic resistance in many bacterial pathogens such as Salmonella has been a major threat for human health. Therefore, identifying the antibiotic resistance patterns of Salmonella serovars may help in treatment of the associated infections.Mohammad Mehdi Soltan DallalMilad AbdiMahya KhalilianZahra RajabiRonak BakhtiariMohammad Kazem Sharifi YazdiSomayeh YaslianifardSeyedeh Masoumeh Abrishamchian LangroudiAlborz University of Medical Sciencesarticlesalmonella foodborne diseases outbreak diarrhea antibiotic susceptibility testing Diseases of the digestive system. GastroenterologyRC799-869ENInternational Journal of Enteric Pathogens, Vol 8, Iss 3, Pp 80-83 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	salmonella foodborne diseases outbreak diarrhea antibiotic susceptibility testing Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	salmonella foodborne diseases outbreak diarrhea antibiotic susceptibility testing Diseases of the digestive system. Gastroenterology RC799-869 Mohammad Mehdi Soltan Dallal Milad Abdi Mahya Khalilian Zahra Rajabi Ronak Bakhtiari Mohammad Kazem Sharifi Yazdi Somayeh Yaslianifard Seyedeh Masoumeh Abrishamchian Langroudi Isolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks
description	Background: Foodborne diseases are a major problem worldwide. The epidemiological investigations in many parts of the world have shown an increase in infections caused by Salmonella serovars. Furthermore, the emergence of drug resistance among them has become a major global concern and awareness of the resistance patterns of Salmonella could be very useful in treatment of diseases. Objective: This study aimed to investigate Salmonella serotypes in foodborne outbreaks by sequencing of ITS region of 16S-23SrRNA gene and to determine their antimicrobial susceptibility pattern. Materials and Methods: A total of 614 diarrheal stool samples were collected from 173 foodborne outbreaks in different provinces of Iran during one year. Identification of Salmonella was carried out by phenotypic and molecular (16s-23srRNA gene detection) methods and antibiotic susceptibility was performed using disc diffusion method. Results: Out of 614 samples, 18 isolates were identified as Salmonella of which 16 (88.9%) isolates were Salmonella Enteritidis and 2 (11.1%) isolates as Salmonella Paratyphi A. All isolates were sensitive to ceftazidime, and high resistance was seen with nalidixic acid with 14 (77.8%) isolates. Conclusion: Increasing antibiotic resistance in many bacterial pathogens such as Salmonella has been a major threat for human health. Therefore, identifying the antibiotic resistance patterns of Salmonella serovars may help in treatment of the associated infections.
format	article
author	Mohammad Mehdi Soltan Dallal Milad Abdi Mahya Khalilian Zahra Rajabi Ronak Bakhtiari Mohammad Kazem Sharifi Yazdi Somayeh Yaslianifard Seyedeh Masoumeh Abrishamchian Langroudi
author_facet	Mohammad Mehdi Soltan Dallal Milad Abdi Mahya Khalilian Zahra Rajabi Ronak Bakhtiari Mohammad Kazem Sharifi Yazdi Somayeh Yaslianifard Seyedeh Masoumeh Abrishamchian Langroudi
author_sort	Mohammad Mehdi Soltan Dallal
title	Isolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks
title_short	Isolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks
title_full	Isolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks
title_fullStr	Isolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks
title_full_unstemmed	Isolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks
title_sort	isolation, identification, and antibiotic susceptibility testing of salmonella isolated from foodborne outbreaks
publisher	Alborz University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/cc05b0f5d64b4da68b30a8bcef98ba74
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Isolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks

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