Molecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan. [version 3; peer review: 3 approved]

Background The presence of Extended-spectrum β-lactamase positive bacteria in hospital setting is an aggravating influential factor for hospitalized patients, and its consequences may be hazardous. Therefore, there is a need for rapid detection methods for newly emerging drug-resistant bacteria. Th...

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Autores principales:	Muzaheed Muzaheed, Naveed Sattar Shaikh, Saeed Sattar Shaikh, Sadananda Acharya, Shajiya Sarwar Moosa, Mohammad Habeeb Shaikh, Faisal M. Alzahrani, Amer Ibrahim Alomar
Formato:	article
Lenguaje:	EN
Publicado:	F1000 Research Ltd 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cc06c03fd97f4ff98783240a18b8208b
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spelling	oai:doaj.org-article:cc06c03fd97f4ff98783240a18b8208b2021-11-22T12:41:06ZMolecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan. [version 3; peer review: 3 approved]2046-140210.12688/f1000research.53221.3https://doaj.org/article/cc06c03fd97f4ff98783240a18b8208b2021-11-01T00:00:00Zhttps://f1000research.com/articles/10-444/v3https://doaj.org/toc/2046-1402Background The presence of Extended-spectrum β-lactamase positive bacteria in hospital setting is an aggravating influential factor for hospitalized patients, and its consequences may be hazardous. Therefore, there is a need for rapid detection methods for newly emerging drug-resistant bacteria. This study was aimed at the molecular characterization of Extended-spectrum β-lactamase -positive Klebsiella pneumoniae isolates recovered from the patients of a teaching hospital in Sindh, Pakistan. Methods A total of 513 K. pneumoniae isolates were obtained from various clinical samples during June 2019 to May 2020. The collected isolates were investigated for antimicrobial susceptibility (antibiogram), and PCR and DNA sequencing were performed to analyse the ESBL genes. Results Among the 513 isolates, as many as 359 (69.9%) were Extended-spectrum β-lactamase producers and 87.5% were multi-drug resistant, while none had resistance to imipenem. PCR scored 3% blaTEM, 3% blaSHV, and 60% blaCTX-M-15 genes for the tested isolates. Conclusion The study showed that CTX-M-15 was the major prevalent Extended-spectrum β-lactamase type among the isolates. Additionally, all the isolates were susceptible to carbapenems. Screening and detection of Extended-spectrum β-lactamase tests are necessary among all isolates from the enterobacteriaceae family in routine microbiology laboratory to prevent associated nosocomial infections. A larger study is essential to understand molecular epidemiology of Extended-spectrum β-lactamase producing organisms to minimize morbidities due to these multidrug resistant organisms.Muzaheed MuzaheedNaveed Sattar ShaikhSaeed Sattar ShaikhSadananda AcharyaShajiya Sarwar MoosaMohammad Habeeb ShaikhFaisal M. AlzahraniAmer Ibrahim AlomarF1000 Research LtdarticleMedicineRScienceQENF1000Research, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muzaheed Muzaheed Naveed Sattar Shaikh Saeed Sattar Shaikh Sadananda Acharya Shajiya Sarwar Moosa Mohammad Habeeb Shaikh Faisal M. Alzahrani Amer Ibrahim Alomar Molecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan. [version 3; peer review: 3 approved]
description	Background The presence of Extended-spectrum β-lactamase positive bacteria in hospital setting is an aggravating influential factor for hospitalized patients, and its consequences may be hazardous. Therefore, there is a need for rapid detection methods for newly emerging drug-resistant bacteria. This study was aimed at the molecular characterization of Extended-spectrum β-lactamase -positive Klebsiella pneumoniae isolates recovered from the patients of a teaching hospital in Sindh, Pakistan. Methods A total of 513 K. pneumoniae isolates were obtained from various clinical samples during June 2019 to May 2020. The collected isolates were investigated for antimicrobial susceptibility (antibiogram), and PCR and DNA sequencing were performed to analyse the ESBL genes. Results Among the 513 isolates, as many as 359 (69.9%) were Extended-spectrum β-lactamase producers and 87.5% were multi-drug resistant, while none had resistance to imipenem. PCR scored 3% blaTEM, 3% blaSHV, and 60% blaCTX-M-15 genes for the tested isolates. Conclusion The study showed that CTX-M-15 was the major prevalent Extended-spectrum β-lactamase type among the isolates. Additionally, all the isolates were susceptible to carbapenems. Screening and detection of Extended-spectrum β-lactamase tests are necessary among all isolates from the enterobacteriaceae family in routine microbiology laboratory to prevent associated nosocomial infections. A larger study is essential to understand molecular epidemiology of Extended-spectrum β-lactamase producing organisms to minimize morbidities due to these multidrug resistant organisms.
format	article
author	Muzaheed Muzaheed Naveed Sattar Shaikh Saeed Sattar Shaikh Sadananda Acharya Shajiya Sarwar Moosa Mohammad Habeeb Shaikh Faisal M. Alzahrani Amer Ibrahim Alomar
author_facet	Muzaheed Muzaheed Naveed Sattar Shaikh Saeed Sattar Shaikh Sadananda Acharya Shajiya Sarwar Moosa Mohammad Habeeb Shaikh Faisal M. Alzahrani Amer Ibrahim Alomar
author_sort	Muzaheed Muzaheed
title	Molecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan. [version 3; peer review: 3 approved]
title_short	Molecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan. [version 3; peer review: 3 approved]
title_full	Molecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan. [version 3; peer review: 3 approved]
title_fullStr	Molecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan. [version 3; peer review: 3 approved]
title_full_unstemmed	Molecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan. [version 3; peer review: 3 approved]
title_sort	molecular epidemiological surveillance of ctx-m-15-producing klebsiella pneumoniae from the patients of a teaching hospital in sindh, pakistan. [version 3; peer review: 3 approved]
publisher	F1000 Research Ltd
publishDate	2021
url	https://doaj.org/article/cc06c03fd97f4ff98783240a18b8208b
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Molecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan. [version 3; peer review: 3 approved]

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