Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation

Abstract Biological systems interact with nanostructured materials on a sub–cellular level. These interactions may govern cell behaviour and the precise control of a nanomaterial's structure and surface chemistry allow for a high degree of tunability to be achieved. Cells are surrounded by an e...

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Autores principales:	Alice C. Taylor, Citlali Helenes González, Benjamin S. Miller, Robert J. Edgington, Patrizia Ferretti, Richard B. Jackman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cc0ddc786f6b4edf914983d18a9643d2
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spelling	oai:doaj.org-article:cc0ddc786f6b4edf914983d18a9643d22021-12-02T15:06:19ZSurface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation10.1038/s41598-017-07361-y2045-2322https://doaj.org/article/cc0ddc786f6b4edf914983d18a9643d22017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07361-yhttps://doaj.org/toc/2045-2322Abstract Biological systems interact with nanostructured materials on a sub–cellular level. These interactions may govern cell behaviour and the precise control of a nanomaterial's structure and surface chemistry allow for a high degree of tunability to be achieved. Cells are surrounded by an extra–cellular matrix with nano–topographical properties. Diamond based materials, and specifically nanostructured diamond has attracted much attention due to its extreme electrical and mechanical properties, chemical inertness and biocompatibility. Here the interaction of nanodiamond monolayers with human Neural Stem Cells (hNSCs) has been investigated. The effect of altering surface functionalisation of nanodiamonds on hNSC adhesion and proliferation has shown that confluent cellular attachment occurs on oxygen terminated nanodiamonds (O–NDs), but not on hydrogen terminated nanodiamonds (H–NDs). Analysis of H and O–NDs by Atomic Force Microscopy, contact angle measurements and protein adsorption suggests that differences in topography, wettability, surface charge and protein adsorption of these surfaces may underlie the difference in cellular adhesion of hNSCs reported here.Alice C. TaylorCitlali Helenes GonzálezBenjamin S. MillerRobert J. EdgingtonPatrizia FerrettiRichard B. JackmanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alice C. Taylor Citlali Helenes González Benjamin S. Miller Robert J. Edgington Patrizia Ferretti Richard B. Jackman Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation
description	Abstract Biological systems interact with nanostructured materials on a sub–cellular level. These interactions may govern cell behaviour and the precise control of a nanomaterial's structure and surface chemistry allow for a high degree of tunability to be achieved. Cells are surrounded by an extra–cellular matrix with nano–topographical properties. Diamond based materials, and specifically nanostructured diamond has attracted much attention due to its extreme electrical and mechanical properties, chemical inertness and biocompatibility. Here the interaction of nanodiamond monolayers with human Neural Stem Cells (hNSCs) has been investigated. The effect of altering surface functionalisation of nanodiamonds on hNSC adhesion and proliferation has shown that confluent cellular attachment occurs on oxygen terminated nanodiamonds (O–NDs), but not on hydrogen terminated nanodiamonds (H–NDs). Analysis of H and O–NDs by Atomic Force Microscopy, contact angle measurements and protein adsorption suggests that differences in topography, wettability, surface charge and protein adsorption of these surfaces may underlie the difference in cellular adhesion of hNSCs reported here.
format	article
author	Alice C. Taylor Citlali Helenes González Benjamin S. Miller Robert J. Edgington Patrizia Ferretti Richard B. Jackman
author_facet	Alice C. Taylor Citlali Helenes González Benjamin S. Miller Robert J. Edgington Patrizia Ferretti Richard B. Jackman
author_sort	Alice C. Taylor
title	Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation
title_short	Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation
title_full	Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation
title_fullStr	Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation
title_full_unstemmed	Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation
title_sort	surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cc0ddc786f6b4edf914983d18a9643d2
work_keys_str_mv	AT alicectaylor surfacefunctionalisationofnanodiamondsforhumanneuralstemcelladhesionandproliferation AT citlalihelenesgonzalez surfacefunctionalisationofnanodiamondsforhumanneuralstemcelladhesionandproliferation AT benjaminsmiller surfacefunctionalisationofnanodiamondsforhumanneuralstemcelladhesionandproliferation AT robertjedgington surfacefunctionalisationofnanodiamondsforhumanneuralstemcelladhesionandproliferation AT patriziaferretti surfacefunctionalisationofnanodiamondsforhumanneuralstemcelladhesionandproliferation AT richardbjackman surfacefunctionalisationofnanodiamondsforhumanneuralstemcelladhesionandproliferation
_version_	1718388541846716416

Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation

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