SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner

Abstract Neurotransmitter release is mediated by the SNARE complex, but the role of its phosphorylation has scarcely been elucidated. Although PKC activators are known to facilitate synaptic transmission, there has been a heated debate on whether PKC mediates facilitation of neurotransmitter release...

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Autores principales:	Norikazu Katayama, Saori Yamamori, Masahiro Fukaya, Shizuka Kobayashi, Masahiko Watanabe, Masami Takahashi, Toshiya Manabe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cc0ee8ec1b914d1793536b31dc7dac10
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spelling	oai:doaj.org-article:cc0ee8ec1b914d1793536b31dc7dac102021-12-02T16:06:56ZSNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner10.1038/s41598-017-08237-x2045-2322https://doaj.org/article/cc0ee8ec1b914d1793536b31dc7dac102017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08237-xhttps://doaj.org/toc/2045-2322Abstract Neurotransmitter release is mediated by the SNARE complex, but the role of its phosphorylation has scarcely been elucidated. Although PKC activators are known to facilitate synaptic transmission, there has been a heated debate on whether PKC mediates facilitation of neurotransmitter release through phosphorylation. One of the SNARE proteins, SNAP-25, is phosphorylated at the residue serine-187 by PKC, but its physiological significance has been unclear. To examine these issues, we analyzed mutant mice lacking the phosphorylation of SNAP-25 serine-187 and found that they exhibited reduced release probability and enhanced presynaptic short-term plasticity, suggesting that not only the release process, but also the dynamics of synaptic vesicles was regulated by the phosphorylation. Furthermore, it has been known that the release probability changes with development, but the precise mechanism has been unclear, and we found that developmental changes in release probability of neurotransmitters were regulated by the phosphorylation. These results indicate that SNAP-25 phosphorylation developmentally facilitates neurotransmitter release but strongly inhibits presynaptic short-term plasticity via modification of the dynamics of synaptic vesicles in presynaptic terminals.Norikazu KatayamaSaori YamamoriMasahiro FukayaShizuka KobayashiMasahiko WatanabeMasami TakahashiToshiya ManabeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Norikazu Katayama Saori Yamamori Masahiro Fukaya Shizuka Kobayashi Masahiko Watanabe Masami Takahashi Toshiya Manabe SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner
description	Abstract Neurotransmitter release is mediated by the SNARE complex, but the role of its phosphorylation has scarcely been elucidated. Although PKC activators are known to facilitate synaptic transmission, there has been a heated debate on whether PKC mediates facilitation of neurotransmitter release through phosphorylation. One of the SNARE proteins, SNAP-25, is phosphorylated at the residue serine-187 by PKC, but its physiological significance has been unclear. To examine these issues, we analyzed mutant mice lacking the phosphorylation of SNAP-25 serine-187 and found that they exhibited reduced release probability and enhanced presynaptic short-term plasticity, suggesting that not only the release process, but also the dynamics of synaptic vesicles was regulated by the phosphorylation. Furthermore, it has been known that the release probability changes with development, but the precise mechanism has been unclear, and we found that developmental changes in release probability of neurotransmitters were regulated by the phosphorylation. These results indicate that SNAP-25 phosphorylation developmentally facilitates neurotransmitter release but strongly inhibits presynaptic short-term plasticity via modification of the dynamics of synaptic vesicles in presynaptic terminals.
format	article
author	Norikazu Katayama Saori Yamamori Masahiro Fukaya Shizuka Kobayashi Masahiko Watanabe Masami Takahashi Toshiya Manabe
author_facet	Norikazu Katayama Saori Yamamori Masahiro Fukaya Shizuka Kobayashi Masahiko Watanabe Masami Takahashi Toshiya Manabe
author_sort	Norikazu Katayama
title	SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner
title_short	SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner
title_full	SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner
title_fullStr	SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner
title_full_unstemmed	SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner
title_sort	snap-25 phosphorylation at ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cc0ee8ec1b914d1793536b31dc7dac10
work_keys_str_mv	AT norikazukatayama snap25phosphorylationatser187regulatessynapticfacilitationandshorttermplasticityinanagedependentmanner AT saoriyamamori snap25phosphorylationatser187regulatessynapticfacilitationandshorttermplasticityinanagedependentmanner AT masahirofukaya snap25phosphorylationatser187regulatessynapticfacilitationandshorttermplasticityinanagedependentmanner AT shizukakobayashi snap25phosphorylationatser187regulatessynapticfacilitationandshorttermplasticityinanagedependentmanner AT masahikowatanabe snap25phosphorylationatser187regulatessynapticfacilitationandshorttermplasticityinanagedependentmanner AT masamitakahashi snap25phosphorylationatser187regulatessynapticfacilitationandshorttermplasticityinanagedependentmanner AT toshiyamanabe snap25phosphorylationatser187regulatessynapticfacilitationandshorttermplasticityinanagedependentmanner
_version_	1718384805724291072

SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner

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