<sup>1</sup>H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations

Cisplatin (cisPt) is an important drug that is used against various cancers, including advanced lung cancer. However, drug resistance is still a major ongoing problem and its investigation is of paramount interest. Here, a high-resolution magic angle spinning (HR-MAS) NMR study is presented decipher...

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Autores principales:	Martina Vermathen, Hendrik von Tengg-Kobligk, Martin Nils Hungerbühler, Peter Vermathen, Nico Ruprecht
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cisplatin cisPt resistance non-small cell lung cancer NSCLC HR-MAS NMR metabolomics Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/cc18b6b72226465cb564fa292e25efdd
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spelling	oai:doaj.org-article:cc18b6b72226465cb564fa292e25efdd2021-11-25T18:27:06Z<sup>1</sup>H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations10.3390/molecules262267661420-3049https://doaj.org/article/cc18b6b72226465cb564fa292e25efdd2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6766https://doaj.org/toc/1420-3049Cisplatin (cisPt) is an important drug that is used against various cancers, including advanced lung cancer. However, drug resistance is still a major ongoing problem and its investigation is of paramount interest. Here, a high-resolution magic angle spinning (HR-MAS) NMR study is presented deciphering the metabolic profile of non-small cell lung cancer (NSCLC) cells and metabolic adaptations at different levels of induced cisPt-resistance, as well as in their de-induced counterparts (cells cultivated in absence of cisPt). In total, fifty-three metabolites were identified and quantified in the <sup>1</sup>H-HR-MAS NMR cell spectra. Metabolic adaptations to cisPt-resistance were detected, which correlated with the degree of resistance. Importantly, de-induced cell lines demonstrated similar metabolic adaptations as the corresponding cisPt-resistant cell lines. Metabolites predominantly changed in cisPt resistant cells and their de-induced counterparts include glutathione and taurine. Characteristic metabolic patterns for cisPt resistance may become relevant as biomarkers in cancer medicine.Martina VermathenHendrik von Tengg-KobligkMartin Nils HungerbühlerPeter VermathenNico RuprechtMDPI AGarticlecisplatincisPt resistancenon-small cell lung cancerNSCLCHR-MAS NMRmetabolomicsOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6766, p 6766 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cisplatin cisPt resistance non-small cell lung cancer NSCLC HR-MAS NMR metabolomics Organic chemistry QD241-441
spellingShingle	cisplatin cisPt resistance non-small cell lung cancer NSCLC HR-MAS NMR metabolomics Organic chemistry QD241-441 Martina Vermathen Hendrik von Tengg-Kobligk Martin Nils Hungerbühler Peter Vermathen Nico Ruprecht <sup>1</sup>H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations
description	Cisplatin (cisPt) is an important drug that is used against various cancers, including advanced lung cancer. However, drug resistance is still a major ongoing problem and its investigation is of paramount interest. Here, a high-resolution magic angle spinning (HR-MAS) NMR study is presented deciphering the metabolic profile of non-small cell lung cancer (NSCLC) cells and metabolic adaptations at different levels of induced cisPt-resistance, as well as in their de-induced counterparts (cells cultivated in absence of cisPt). In total, fifty-three metabolites were identified and quantified in the <sup>1</sup>H-HR-MAS NMR cell spectra. Metabolic adaptations to cisPt-resistance were detected, which correlated with the degree of resistance. Importantly, de-induced cell lines demonstrated similar metabolic adaptations as the corresponding cisPt-resistant cell lines. Metabolites predominantly changed in cisPt resistant cells and their de-induced counterparts include glutathione and taurine. Characteristic metabolic patterns for cisPt resistance may become relevant as biomarkers in cancer medicine.
format	article
author	Martina Vermathen Hendrik von Tengg-Kobligk Martin Nils Hungerbühler Peter Vermathen Nico Ruprecht
author_facet	Martina Vermathen Hendrik von Tengg-Kobligk Martin Nils Hungerbühler Peter Vermathen Nico Ruprecht
author_sort	Martina Vermathen
title	<sup>1</sup>H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations
title_short	<sup>1</sup>H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations
title_full	<sup>1</sup>H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations
title_fullStr	<sup>1</sup>H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations
title_full_unstemmed	<sup>1</sup>H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations
title_sort	<sup>1</sup>h hr-mas nmr based metabolic profiling of lung cancer cells with induced and de-induced cisplatin resistance to reveal metabolic resistance adaptations
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cc18b6b72226465cb564fa292e25efdd
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<sup>1</sup>H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations

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