Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation

Several significant advancements in the field of bone regenerative medicine have been made in recent years. However, therapeutic options, such as bone grafts, have several drawbacks. There is a need to develop an adequate bone substitute. As a result, significant bone defects/injuries pose a severe...

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Autores principales:	Muhammad Umar Aslam Khan, Saiful Izwan Abd Razak, Mohamed Nainar Mohamed Ansari, Razauden Mohamed Zulkifli, Nurliyana Ahmad Zawawi, Muhammad Arshad
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biopolymer biomaterials biodegradations polysaccharide bone tissue engineering Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/cc195d0033b14b468233d2d515c0acba
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spelling	oai:doaj.org-article:cc195d0033b14b468233d2d515c0acba2021-11-11T18:41:16ZDevelopment of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation10.3390/polym132136112073-4360https://doaj.org/article/cc195d0033b14b468233d2d515c0acba2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3611https://doaj.org/toc/2073-4360Several significant advancements in the field of bone regenerative medicine have been made in recent years. However, therapeutic options, such as bone grafts, have several drawbacks. There is a need to develop an adequate bone substitute. As a result, significant bone defects/injuries pose a severe challenge for orthopaedic and reconstructive bone tissue. We synthesized polymeric composite material from arabinoxylan (ARX), β-glucan (BG), nano-hydroxyapatite (nHAp), graphene oxide (GO), acrylic acid (AAc) through free radical polymerization and porous scaffold fabricated using the freeze-drying technique. These fabricated porous scaffolds were then coated with chitosan solution to enhance their biological activities. The complex structure of BG, nHAp, GO was studied through various characterization and biological assays. The structural, morphological, wetting and mechanical analyses were determined using FT-IR, XRD, XPS, SEM/EXD, water contact angle and UTM. The swelling (aqueous and PBS media) and degradation (PBS media) observed their behavior in contact with body fluid. The biological activities were conducted against mouse pre-osteoblast cell lines. The result found that BGH3 has desirable morphological, structural with optimum swelling, degradation, and mechanical behavior. It was also found to be cytocompatible against MC3T3-E1 cell lines. The obtained results confirmed that the fabricated polymeric scaffolds would be a potential bone substitute to regenerate defective bone with different loading bearing applications for bone tissue engineering.Muhammad Umar Aslam KhanSaiful Izwan Abd RazakMohamed Nainar Mohamed AnsariRazauden Mohamed ZulkifliNurliyana Ahmad ZawawiMuhammad ArshadMDPI AGarticlebiopolymerbiomaterialsbiodegradationspolysaccharidebone tissue engineeringOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3611, p 3611 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biopolymer biomaterials biodegradations polysaccharide bone tissue engineering Organic chemistry QD241-441
spellingShingle	biopolymer biomaterials biodegradations polysaccharide bone tissue engineering Organic chemistry QD241-441 Muhammad Umar Aslam Khan Saiful Izwan Abd Razak Mohamed Nainar Mohamed Ansari Razauden Mohamed Zulkifli Nurliyana Ahmad Zawawi Muhammad Arshad Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
description	Several significant advancements in the field of bone regenerative medicine have been made in recent years. However, therapeutic options, such as bone grafts, have several drawbacks. There is a need to develop an adequate bone substitute. As a result, significant bone defects/injuries pose a severe challenge for orthopaedic and reconstructive bone tissue. We synthesized polymeric composite material from arabinoxylan (ARX), β-glucan (BG), nano-hydroxyapatite (nHAp), graphene oxide (GO), acrylic acid (AAc) through free radical polymerization and porous scaffold fabricated using the freeze-drying technique. These fabricated porous scaffolds were then coated with chitosan solution to enhance their biological activities. The complex structure of BG, nHAp, GO was studied through various characterization and biological assays. The structural, morphological, wetting and mechanical analyses were determined using FT-IR, XRD, XPS, SEM/EXD, water contact angle and UTM. The swelling (aqueous and PBS media) and degradation (PBS media) observed their behavior in contact with body fluid. The biological activities were conducted against mouse pre-osteoblast cell lines. The result found that BGH3 has desirable morphological, structural with optimum swelling, degradation, and mechanical behavior. It was also found to be cytocompatible against MC3T3-E1 cell lines. The obtained results confirmed that the fabricated polymeric scaffolds would be a potential bone substitute to regenerate defective bone with different loading bearing applications for bone tissue engineering.
format	article
author	Muhammad Umar Aslam Khan Saiful Izwan Abd Razak Mohamed Nainar Mohamed Ansari Razauden Mohamed Zulkifli Nurliyana Ahmad Zawawi Muhammad Arshad
author_facet	Muhammad Umar Aslam Khan Saiful Izwan Abd Razak Mohamed Nainar Mohamed Ansari Razauden Mohamed Zulkifli Nurliyana Ahmad Zawawi Muhammad Arshad
author_sort	Muhammad Umar Aslam Khan
title	Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title_short	Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title_full	Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title_fullStr	Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title_full_unstemmed	Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title_sort	development of biodegradable bio-based composite for bone tissue engineering: synthesis, characterization and in vitro biocompatible evaluation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cc195d0033b14b468233d2d515c0acba
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Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation

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