Ethanol Extraction of Polar Lipids from <i>Nannochloropsis oceanica</i> for Food, Feed, and Biotechnology Applications Evaluated Using Lipidomic Approaches

<i>Nannochloropsis oceanica</i> can accumulate lipids and is a good source of polar lipids, which are emerging as new value-added compounds with high commercial value for the food, nutraceutical, and pharmaceutical industries. Some applications may limit the extraction solvents, such as...

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Autores principales:	Tânia Melo, Ana R. P. Figueiredo, Elisabete da Costa, Daniela Couto, Joana Silva, M. Rosário Domingues, Pedro Domingues
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Nannochloropsis oceanica</i> solvent extraction lipidomics glycolipids phospholipids betaine lipids Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cc1affb9686e400d8cd9e9389d9810be
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spelling	oai:doaj.org-article:cc1affb9686e400d8cd9e9389d9810be2021-11-25T18:12:39ZEthanol Extraction of Polar Lipids from <i>Nannochloropsis oceanica</i> for Food, Feed, and Biotechnology Applications Evaluated Using Lipidomic Approaches10.3390/md191105931660-3397https://doaj.org/article/cc1affb9686e400d8cd9e9389d9810be2021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/593https://doaj.org/toc/1660-3397<i>Nannochloropsis oceanica</i> can accumulate lipids and is a good source of polar lipids, which are emerging as new value-added compounds with high commercial value for the food, nutraceutical, and pharmaceutical industries. Some applications may limit the extraction solvents, such as food applications that require safe food-grade solvents, such as ethanol. However, the effect of using ethanol as an extraction solvent on the quality of the extracted polar lipidome, compared to other more traditional methods, is not yet well established. In this study, the polar lipid profile of <i>N. oceanica</i> extracts was obtained using different solvents, including chloroform/methanol (CM), dichloromethane/methanol (DM), dichloromethane/ethanol (DE), and ethanol (E), and evaluated by modern lipidomic methods using LC-MS/MS. Ultrasonic bath (E + USB)- and ultrasonic probe (E + USP)-assisted methodologies were implemented to increase the lipid extraction yields using ethanol. The polar lipid signature and antioxidant activity of DM, E + USB, and E + USP resemble conventional CM, demonstrating a similar extraction efficiency, while the DE and ethanol extracts were significantly different. Our results showed the impact of different extraction solvents in the polar lipid composition of the final extracts and demonstrated the feasibility of E + USB and E + USP as safe and food-grade sources of polar lipids, with the potential for high-added-value biotechnological applications.Tânia MeloAna R. P. FigueiredoElisabete da CostaDaniela CoutoJoana SilvaM. Rosário DominguesPedro DominguesMDPI AGarticle<i>Nannochloropsis oceanica</i>solvent extractionlipidomicsglycolipidsphospholipidsbetaine lipidsBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 593, p 593 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Nannochloropsis oceanica</i> solvent extraction lipidomics glycolipids phospholipids betaine lipids Biology (General) QH301-705.5
spellingShingle	<i>Nannochloropsis oceanica</i> solvent extraction lipidomics glycolipids phospholipids betaine lipids Biology (General) QH301-705.5 Tânia Melo Ana R. P. Figueiredo Elisabete da Costa Daniela Couto Joana Silva M. Rosário Domingues Pedro Domingues Ethanol Extraction of Polar Lipids from <i>Nannochloropsis oceanica</i> for Food, Feed, and Biotechnology Applications Evaluated Using Lipidomic Approaches
description	<i>Nannochloropsis oceanica</i> can accumulate lipids and is a good source of polar lipids, which are emerging as new value-added compounds with high commercial value for the food, nutraceutical, and pharmaceutical industries. Some applications may limit the extraction solvents, such as food applications that require safe food-grade solvents, such as ethanol. However, the effect of using ethanol as an extraction solvent on the quality of the extracted polar lipidome, compared to other more traditional methods, is not yet well established. In this study, the polar lipid profile of <i>N. oceanica</i> extracts was obtained using different solvents, including chloroform/methanol (CM), dichloromethane/methanol (DM), dichloromethane/ethanol (DE), and ethanol (E), and evaluated by modern lipidomic methods using LC-MS/MS. Ultrasonic bath (E + USB)- and ultrasonic probe (E + USP)-assisted methodologies were implemented to increase the lipid extraction yields using ethanol. The polar lipid signature and antioxidant activity of DM, E + USB, and E + USP resemble conventional CM, demonstrating a similar extraction efficiency, while the DE and ethanol extracts were significantly different. Our results showed the impact of different extraction solvents in the polar lipid composition of the final extracts and demonstrated the feasibility of E + USB and E + USP as safe and food-grade sources of polar lipids, with the potential for high-added-value biotechnological applications.
format	article
author	Tânia Melo Ana R. P. Figueiredo Elisabete da Costa Daniela Couto Joana Silva M. Rosário Domingues Pedro Domingues
author_facet	Tânia Melo Ana R. P. Figueiredo Elisabete da Costa Daniela Couto Joana Silva M. Rosário Domingues Pedro Domingues
author_sort	Tânia Melo
title	Ethanol Extraction of Polar Lipids from <i>Nannochloropsis oceanica</i> for Food, Feed, and Biotechnology Applications Evaluated Using Lipidomic Approaches
title_short	Ethanol Extraction of Polar Lipids from <i>Nannochloropsis oceanica</i> for Food, Feed, and Biotechnology Applications Evaluated Using Lipidomic Approaches
title_full	Ethanol Extraction of Polar Lipids from <i>Nannochloropsis oceanica</i> for Food, Feed, and Biotechnology Applications Evaluated Using Lipidomic Approaches
title_fullStr	Ethanol Extraction of Polar Lipids from <i>Nannochloropsis oceanica</i> for Food, Feed, and Biotechnology Applications Evaluated Using Lipidomic Approaches
title_full_unstemmed	Ethanol Extraction of Polar Lipids from <i>Nannochloropsis oceanica</i> for Food, Feed, and Biotechnology Applications Evaluated Using Lipidomic Approaches
title_sort	ethanol extraction of polar lipids from <i>nannochloropsis oceanica</i> for food, feed, and biotechnology applications evaluated using lipidomic approaches
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cc1affb9686e400d8cd9e9389d9810be
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Ethanol Extraction of Polar Lipids from <i>Nannochloropsis oceanica</i> for Food, Feed, and Biotechnology Applications Evaluated Using Lipidomic Approaches

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