Virulence Comparison of Salmonella enterica Subsp. enterica Isolates from Chicken and Whole Genome Analysis of the High Virulent Strain S. Enteritidis 211

Background: Salmonellaenterica is one of the common pathogens in both humans and animals that causes salmonellosis and threatens public health all over the world. Methods and Results: Here we determined the virulence phenotypes of nine Salmonella</i&...

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Autores principales:	Luqing Cui, Xiangru Wang, Yue Zhao, Zhong Peng, Pan Gao, Zhengzheng Cao, Jiawei Feng, Fan Zhang, Kaixuan Guo, Min Wu, Huanchun Chen, Menghong Dai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Salmonella enterica</i> chicken virulence whole genome sequencing <i>S</i>. Enteritidis comparative genomic analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cc27b3b50585454485dbe4050964ba12
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spelling	oai:doaj.org-article:cc27b3b50585454485dbe4050964ba122021-11-25T18:24:30ZVirulence Comparison of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Isolates from Chicken and Whole Genome Analysis of the High Virulent Strain <i>S</i>. Enteritidis 21110.3390/microorganisms91122392076-2607https://doaj.org/article/cc27b3b50585454485dbe4050964ba122021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2239https://doaj.org/toc/2076-2607Background: <i>Salmonella</i><i>enterica</i> is one of the common pathogens in both humans and animals that causes salmonellosis and threatens public health all over the world. Methods and Results: Here we determined the virulence phenotypes of nine <i>Salmonella</i><i>enterica</i> subsp. <i>enterica</i> (<i>S. enterica</i>) isolates in vitro and in vivo, including pathogenicity to chicken, cell infection, biofilm formation and virulence gene expressions. <i>S</i>. Enteritidis 211 (SE211) was highly pathogenic with notable virulence features among the nine isolates. The combination of multiple virulence genes contributed to the conferring of the high virulence in SE211. Importantly, many mobile genetic elements (MGEs) were found in the genome sequence of SE211, including a virulence plasmid, genomic islands, and prophage regions. The MGEs and CRISPR-Cas system might function synergistically for gene transfer and immune defense. In addition, the neighbor joining tree and the minimum spanning tree were constructed in this study. Conclusions: This study provided both the virulence phenotypes and genomic features, which might contribute to the understanding of bacterial virulence mechanisms in <i>Salmonella enterica</i> subsp. <i>enterica</i>. The first completed genomic sequence for the high virulent <i>S</i>. Enteritidis isolate SE211 and the comparative genomics and phylogenetic analyses provided a preliminary understanding of <i>S. enterica</i> genetics and laid the foundation for further study.Luqing CuiXiangru WangYue ZhaoZhong PengPan GaoZhengzheng CaoJiawei FengFan ZhangKaixuan GuoMin WuHuanchun ChenMenghong DaiMDPI AGarticle<i>Salmonella enterica</i>chickenvirulencewhole genome sequencing<i>S</i>. Enteritidiscomparative genomic analysisBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2239, p 2239 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Salmonella enterica</i> chicken virulence whole genome sequencing <i>S</i>. Enteritidis comparative genomic analysis Biology (General) QH301-705.5
spellingShingle	<i>Salmonella enterica</i> chicken virulence whole genome sequencing <i>S</i>. Enteritidis comparative genomic analysis Biology (General) QH301-705.5 Luqing Cui Xiangru Wang Yue Zhao Zhong Peng Pan Gao Zhengzheng Cao Jiawei Feng Fan Zhang Kaixuan Guo Min Wu Huanchun Chen Menghong Dai Virulence Comparison of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Isolates from Chicken and Whole Genome Analysis of the High Virulent Strain <i>S</i>. Enteritidis 211
description	Background: <i>Salmonella</i><i>enterica</i> is one of the common pathogens in both humans and animals that causes salmonellosis and threatens public health all over the world. Methods and Results: Here we determined the virulence phenotypes of nine <i>Salmonella</i><i>enterica</i> subsp. <i>enterica</i> (<i>S. enterica</i>) isolates in vitro and in vivo, including pathogenicity to chicken, cell infection, biofilm formation and virulence gene expressions. <i>S</i>. Enteritidis 211 (SE211) was highly pathogenic with notable virulence features among the nine isolates. The combination of multiple virulence genes contributed to the conferring of the high virulence in SE211. Importantly, many mobile genetic elements (MGEs) were found in the genome sequence of SE211, including a virulence plasmid, genomic islands, and prophage regions. The MGEs and CRISPR-Cas system might function synergistically for gene transfer and immune defense. In addition, the neighbor joining tree and the minimum spanning tree were constructed in this study. Conclusions: This study provided both the virulence phenotypes and genomic features, which might contribute to the understanding of bacterial virulence mechanisms in <i>Salmonella enterica</i> subsp. <i>enterica</i>. The first completed genomic sequence for the high virulent <i>S</i>. Enteritidis isolate SE211 and the comparative genomics and phylogenetic analyses provided a preliminary understanding of <i>S. enterica</i> genetics and laid the foundation for further study.
format	article
author	Luqing Cui Xiangru Wang Yue Zhao Zhong Peng Pan Gao Zhengzheng Cao Jiawei Feng Fan Zhang Kaixuan Guo Min Wu Huanchun Chen Menghong Dai
author_facet	Luqing Cui Xiangru Wang Yue Zhao Zhong Peng Pan Gao Zhengzheng Cao Jiawei Feng Fan Zhang Kaixuan Guo Min Wu Huanchun Chen Menghong Dai
author_sort	Luqing Cui
title	Virulence Comparison of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Isolates from Chicken and Whole Genome Analysis of the High Virulent Strain <i>S</i>. Enteritidis 211
title_short	Virulence Comparison of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Isolates from Chicken and Whole Genome Analysis of the High Virulent Strain <i>S</i>. Enteritidis 211
title_full	Virulence Comparison of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Isolates from Chicken and Whole Genome Analysis of the High Virulent Strain <i>S</i>. Enteritidis 211
title_fullStr	Virulence Comparison of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Isolates from Chicken and Whole Genome Analysis of the High Virulent Strain <i>S</i>. Enteritidis 211
title_full_unstemmed	Virulence Comparison of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Isolates from Chicken and Whole Genome Analysis of the High Virulent Strain <i>S</i>. Enteritidis 211
title_sort	virulence comparison of <i>salmonella enterica</i> subsp. <i>enterica</i> isolates from chicken and whole genome analysis of the high virulent strain <i>s</i>. enteritidis 211
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cc27b3b50585454485dbe4050964ba12
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Virulence Comparison of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Isolates from Chicken and Whole Genome Analysis of the High Virulent Strain <i>S</i>. Enteritidis 211

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