First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)

Abstract. Pissaparn M, Phimphan S, Chaiyasan P, Tanoamtong A, Liehr T, Suwannapoom C, Reungsing M, Supiwong W. 2020. First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866). Biodiversitas 21: 4309-4316. Here first analysis of chromosomes and nucleolar organizer region (...

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Autores principales:	MANACHAYA PISSAPARN, SUMALEE PHIMPHAN, PATCHARAPORN CHAIYASAN, ALONGKLOD TANOAMTONG, THOMAS LIEHR, CHATMONGKON SUWANNAPOOM, MONTRI REUNGSING, Weerayuth Supiwong
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	chromosome, fish, karyotype, nucleolar organizer region, pao cochinchinensis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cc2ceabc7d814309b8fa6a76edebb14b
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spelling	oai:doaj.org-article:cc2ceabc7d814309b8fa6a76edebb14b2021-11-22T00:41:43ZFirst chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)1412-033X2085-472210.13057/biodiv/d210949https://doaj.org/article/cc2ceabc7d814309b8fa6a76edebb14b2020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6003https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Pissaparn M, Phimphan S, Chaiyasan P, Tanoamtong A, Liehr T, Suwannapoom C, Reungsing M, Supiwong W. 2020. First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866). Biodiversitas 21: 4309-4316. Here first analysis of chromosomes and nucleolar organizer region (NOR) pattern in pufferfish Pao cochinchinensis (Steindachner, 1866) was undertaken. Chromosomal preparations were obtained from kidney of P. cochinchinensis from Chi River basin in Thailand. Chromosomal characteristics were analyzed by Giemsa staining, Ag-NOR banding as well as fluorescence in situ hybridization (FISH) using microsatellites d(CA)15 and d(CGG)10 probes. P. cochinchinensis had 2n = 40 with the fundamental number (NF) 74, both in male and female. The karyotype exhibited 12 metacentric (m), 10 submetacentric (sm), 12 acrocentric (a) and 6 telocentric (t) chromosomes. No differentiated heteromorphic sex chromosomes were observed. NORs were located on short arms adjacent to telomere of the metacentric chromosome pair 4, which coincide with signals of d(CGG)10 probe. FISH with d(CGG)10 sequences were also displayed at the telomeres of most other chromosomes, whereas d(CA)15 repeats highly accumulated throughout almost all entire chromosomes except for centromeric regions. The results of conventional Giemsa staining presented the differentiation even the same genus. The localization of NORs on one pair of chromosomes only is a common characteristic found in many fish groups as well as other vertebrates. Mapping of two distinct microsatellites demonstrated the remarkable chromosomal diversification that characterizes evolution in the genus Pao. Both, conventional and molecular cytogenetics are excellent tools to study, and better understand chromosomal evolution, as well as to uncover biodiversity among fishes.MANACHAYA PISSAPARNSUMALEE PHIMPHANPATCHARAPORN CHAIYASANALONGKLOD TANOAMTONGTHOMAS LIEHRCHATMONGKON SUWANNAPOOMMONTRI REUNGSINGWeerayuth SupiwongMBI & UNS Soloarticlechromosome, fish, karyotype, nucleolar organizer region, pao cochinchinensisBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	chromosome, fish, karyotype, nucleolar organizer region, pao cochinchinensis Biology (General) QH301-705.5
spellingShingle	chromosome, fish, karyotype, nucleolar organizer region, pao cochinchinensis Biology (General) QH301-705.5 MANACHAYA PISSAPARN SUMALEE PHIMPHAN PATCHARAPORN CHAIYASAN ALONGKLOD TANOAMTONG THOMAS LIEHR CHATMONGKON SUWANNAPOOM MONTRI REUNGSING Weerayuth Supiwong First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)
description	Abstract. Pissaparn M, Phimphan S, Chaiyasan P, Tanoamtong A, Liehr T, Suwannapoom C, Reungsing M, Supiwong W. 2020. First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866). Biodiversitas 21: 4309-4316. Here first analysis of chromosomes and nucleolar organizer region (NOR) pattern in pufferfish Pao cochinchinensis (Steindachner, 1866) was undertaken. Chromosomal preparations were obtained from kidney of P. cochinchinensis from Chi River basin in Thailand. Chromosomal characteristics were analyzed by Giemsa staining, Ag-NOR banding as well as fluorescence in situ hybridization (FISH) using microsatellites d(CA)15 and d(CGG)10 probes. P. cochinchinensis had 2n = 40 with the fundamental number (NF) 74, both in male and female. The karyotype exhibited 12 metacentric (m), 10 submetacentric (sm), 12 acrocentric (a) and 6 telocentric (t) chromosomes. No differentiated heteromorphic sex chromosomes were observed. NORs were located on short arms adjacent to telomere of the metacentric chromosome pair 4, which coincide with signals of d(CGG)10 probe. FISH with d(CGG)10 sequences were also displayed at the telomeres of most other chromosomes, whereas d(CA)15 repeats highly accumulated throughout almost all entire chromosomes except for centromeric regions. The results of conventional Giemsa staining presented the differentiation even the same genus. The localization of NORs on one pair of chromosomes only is a common characteristic found in many fish groups as well as other vertebrates. Mapping of two distinct microsatellites demonstrated the remarkable chromosomal diversification that characterizes evolution in the genus Pao. Both, conventional and molecular cytogenetics are excellent tools to study, and better understand chromosomal evolution, as well as to uncover biodiversity among fishes.
format	article
author	MANACHAYA PISSAPARN SUMALEE PHIMPHAN PATCHARAPORN CHAIYASAN ALONGKLOD TANOAMTONG THOMAS LIEHR CHATMONGKON SUWANNAPOOM MONTRI REUNGSING Weerayuth Supiwong
author_facet	MANACHAYA PISSAPARN SUMALEE PHIMPHAN PATCHARAPORN CHAIYASAN ALONGKLOD TANOAMTONG THOMAS LIEHR CHATMONGKON SUWANNAPOOM MONTRI REUNGSING Weerayuth Supiwong
author_sort	MANACHAYA PISSAPARN
title	First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)
title_short	First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)
title_full	First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)
title_fullStr	First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)
title_full_unstemmed	First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)
title_sort	first chromosome analysis of thai pufferfish pao cochinchinensis (steindachner, 1866)
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/cc2ceabc7d814309b8fa6a76edebb14b
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First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)

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