Characterisation of reduction state of cystine linkages on wool fibre surface under heterogeneous reaction conditions

As a natural biomaterial with outstanding properties, keratin fibres hold promising potential in many application fields. The reduction of disulphides in keratin to the corresponding thiols plays a central role e.g. in modification and functionalisation of wool, in hair cosmetics, as well as in adva...

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Autores principales:	Madita Buchacher, Thomas Bechtold, Tung Pham
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Keratin fibres Heterogeneous thiol determination Cystine Disulphide Wool Polymers and polymer manufacture TP1080-1185
Acceso en línea:	https://doaj.org/article/cc32ef8cc6bb489eb1f58b44e1c03be9
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spelling	oai:doaj.org-article:cc32ef8cc6bb489eb1f58b44e1c03be92021-11-24T04:26:39ZCharacterisation of reduction state of cystine linkages on wool fibre surface under heterogeneous reaction conditions0142-941810.1016/j.polymertesting.2021.107438https://doaj.org/article/cc32ef8cc6bb489eb1f58b44e1c03be92022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0142941821003810https://doaj.org/toc/0142-9418As a natural biomaterial with outstanding properties, keratin fibres hold promising potential in many application fields. The reduction of disulphides in keratin to the corresponding thiols plays a central role e.g. in modification and functionalisation of wool, in hair cosmetics, as well as in advanced applications using keratin as biomaterial. To achieve controlled conditions, the need for a quantitative determination of thiols on the solid wool fibre is evident. This work presents an advanced method of heterogeneous potentiometric back titration method for thiol detection in intact keratin fibres. First, keratin is reductively treated with NaBH4, then the thiol content in keratin is determined using K3[Fe(CN)6] and K2CrO4 as reagents and potentiometric endpoint detection. Thiol concentration in the range between 6.83 and 26.17 (±2.13) μmol/g can be detected with the heterogeneous back titration. Thus, the method is simple in execution and reliable in performance and of significant potential, both as scientific technique to quantify thiols in insoluble keratin material, as well as for process and quality control in industry.Madita BuchacherThomas BechtoldTung PhamElsevierarticleKeratin fibresHeterogeneous thiol determinationCystineDisulphideWoolPolymers and polymer manufactureTP1080-1185ENPolymer Testing, Vol 106, Iss , Pp 107438- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Keratin fibres Heterogeneous thiol determination Cystine Disulphide Wool Polymers and polymer manufacture TP1080-1185
spellingShingle	Keratin fibres Heterogeneous thiol determination Cystine Disulphide Wool Polymers and polymer manufacture TP1080-1185 Madita Buchacher Thomas Bechtold Tung Pham Characterisation of reduction state of cystine linkages on wool fibre surface under heterogeneous reaction conditions
description	As a natural biomaterial with outstanding properties, keratin fibres hold promising potential in many application fields. The reduction of disulphides in keratin to the corresponding thiols plays a central role e.g. in modification and functionalisation of wool, in hair cosmetics, as well as in advanced applications using keratin as biomaterial. To achieve controlled conditions, the need for a quantitative determination of thiols on the solid wool fibre is evident. This work presents an advanced method of heterogeneous potentiometric back titration method for thiol detection in intact keratin fibres. First, keratin is reductively treated with NaBH4, then the thiol content in keratin is determined using K3[Fe(CN)6] and K2CrO4 as reagents and potentiometric endpoint detection. Thiol concentration in the range between 6.83 and 26.17 (±2.13) μmol/g can be detected with the heterogeneous back titration. Thus, the method is simple in execution and reliable in performance and of significant potential, both as scientific technique to quantify thiols in insoluble keratin material, as well as for process and quality control in industry.
format	article
author	Madita Buchacher Thomas Bechtold Tung Pham
author_facet	Madita Buchacher Thomas Bechtold Tung Pham
author_sort	Madita Buchacher
title	Characterisation of reduction state of cystine linkages on wool fibre surface under heterogeneous reaction conditions
title_short	Characterisation of reduction state of cystine linkages on wool fibre surface under heterogeneous reaction conditions
title_full	Characterisation of reduction state of cystine linkages on wool fibre surface under heterogeneous reaction conditions
title_fullStr	Characterisation of reduction state of cystine linkages on wool fibre surface under heterogeneous reaction conditions
title_full_unstemmed	Characterisation of reduction state of cystine linkages on wool fibre surface under heterogeneous reaction conditions
title_sort	characterisation of reduction state of cystine linkages on wool fibre surface under heterogeneous reaction conditions
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/cc32ef8cc6bb489eb1f58b44e1c03be9
work_keys_str_mv	AT maditabuchacher characterisationofreductionstateofcystinelinkagesonwoolfibresurfaceunderheterogeneousreactionconditions AT thomasbechtold characterisationofreductionstateofcystinelinkagesonwoolfibresurfaceunderheterogeneousreactionconditions AT tungpham characterisationofreductionstateofcystinelinkagesonwoolfibresurfaceunderheterogeneousreactionconditions
_version_	1718416001994850304

Characterisation of reduction state of cystine linkages on wool fibre surface under heterogeneous reaction conditions

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