Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen

Background: In artificial insemination, chicken egg yolk is added to bovine semen to protect it during the cryopreservation process, although it contains substances that can affect the microbiological quality and metabolism of sperm. Objective: To evaluate post-thaw quality of bovine cryopreserved s...

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Autores principales:	Elizabeth Varela-Giraldo, Alexandra Úsuga-Suárez, Juan E. Duque-Cortés, Jorge Gómez-Oquendo, Giovanni Restrepo-Betancur
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2020
Materias:	bull cryopreservation cryoprotectant egg yolk extender low-density lipoproteins semen trehalose Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/cc3d4baca58c4afab5a886d0e09c20d2
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spelling	oai:doaj.org-article:cc3d4baca58c4afab5a886d0e09c20d22021-12-01T15:02:45ZEffect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen2256-295810.17533/udea.rccp.v34n3a03https://doaj.org/article/cc3d4baca58c4afab5a886d0e09c20d22020-08-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/343399https://doaj.org/toc/2256-2958Background: In artificial insemination, chicken egg yolk is added to bovine semen to protect it during the cryopreservation process, although it contains substances that can affect the microbiological quality and metabolism of sperm. Objective: To evaluate post-thaw quality of bovine cryopreserved semen added with centrifuged and non-centrifuged egg yolk, low-density lipoproteins (LDL), and trehalose (T). Methods: Ten ejaculates from five bulls were cryopreserved under the treatments T1: pure egg yolk (PEY) at 20% v/v, T2: centrifuged egg yolk (CEY) at 20% v/v, T3: LDL at 8% v/v, T4: T at 100 mM, and T5: T at 100 mM plus LDL at 8% v/v (TLDL). Spermatic motility and kinetics, functional membrane integrity (FMI), structural membrane integrity (SMI), sperm vitality (SV) and abnormal morphology (AM) were assessed using the Sperm Class Analyzer (SCA®) system, hypoosmotic test (HOST), SYBR/PI probes, and eosin–nigrosin staining, respectively. A completely randomized design was used. Normal distribution of the variables was validated through the Kolmogórov– Smirnov test. A generalized linear model was used to determine sources of variation. Means were compared using the Tukey test. Results: Inclusion of CEY or LDL had a similar effect on sperm protection, and were superior for motility, kinetics and membrane integrity compared to the other treatments (p<0.05). CEY was superior for progressive motility (p<0.05). The cryoprotective action of LDL was similar to TLDL for motility and kinetics, SMI, SV, and AM (p<0.05). Inclusion of PEY and T resulted in the lowest semen quality (p<0.05). The use of T resulted in a reduction in FMI and SMI (p<0.05). No differences in AM between treatments were found (p>0.05). Conclusions: Egg yolk can be replaced by centrifuged egg yolk or low-density lipoproteins in the freezing extender used for bovine semen used in artificial insemination.Elizabeth Varela-GiraldoAlexandra Úsuga-SuárezJuan E. Duque-CortésJorge Gómez-OquendoGiovanni Restrepo-BetancurUniversidad de Antioquiaarticlebullcryopreservationcryoprotectantegg yolkextenderlow-density lipoproteinssementrehaloseAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 34, Iss 3, Pp 200-211 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bull cryopreservation cryoprotectant egg yolk extender low-density lipoproteins semen trehalose Animal culture SF1-1100
spellingShingle	bull cryopreservation cryoprotectant egg yolk extender low-density lipoproteins semen trehalose Animal culture SF1-1100 Elizabeth Varela-Giraldo Alexandra Úsuga-Suárez Juan E. Duque-Cortés Jorge Gómez-Oquendo Giovanni Restrepo-Betancur Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen
description	Background: In artificial insemination, chicken egg yolk is added to bovine semen to protect it during the cryopreservation process, although it contains substances that can affect the microbiological quality and metabolism of sperm. Objective: To evaluate post-thaw quality of bovine cryopreserved semen added with centrifuged and non-centrifuged egg yolk, low-density lipoproteins (LDL), and trehalose (T). Methods: Ten ejaculates from five bulls were cryopreserved under the treatments T1: pure egg yolk (PEY) at 20% v/v, T2: centrifuged egg yolk (CEY) at 20% v/v, T3: LDL at 8% v/v, T4: T at 100 mM, and T5: T at 100 mM plus LDL at 8% v/v (TLDL). Spermatic motility and kinetics, functional membrane integrity (FMI), structural membrane integrity (SMI), sperm vitality (SV) and abnormal morphology (AM) were assessed using the Sperm Class Analyzer (SCA®) system, hypoosmotic test (HOST), SYBR/PI probes, and eosin–nigrosin staining, respectively. A completely randomized design was used. Normal distribution of the variables was validated through the Kolmogórov– Smirnov test. A generalized linear model was used to determine sources of variation. Means were compared using the Tukey test. Results: Inclusion of CEY or LDL had a similar effect on sperm protection, and were superior for motility, kinetics and membrane integrity compared to the other treatments (p<0.05). CEY was superior for progressive motility (p<0.05). The cryoprotective action of LDL was similar to TLDL for motility and kinetics, SMI, SV, and AM (p<0.05). Inclusion of PEY and T resulted in the lowest semen quality (p<0.05). The use of T resulted in a reduction in FMI and SMI (p<0.05). No differences in AM between treatments were found (p>0.05). Conclusions: Egg yolk can be replaced by centrifuged egg yolk or low-density lipoproteins in the freezing extender used for bovine semen used in artificial insemination.
format	article
author	Elizabeth Varela-Giraldo Alexandra Úsuga-Suárez Juan E. Duque-Cortés Jorge Gómez-Oquendo Giovanni Restrepo-Betancur
author_facet	Elizabeth Varela-Giraldo Alexandra Úsuga-Suárez Juan E. Duque-Cortés Jorge Gómez-Oquendo Giovanni Restrepo-Betancur
author_sort	Elizabeth Varela-Giraldo
title	Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen
title_short	Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen
title_full	Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen
title_fullStr	Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen
title_full_unstemmed	Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen
title_sort	effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen
publisher	Universidad de Antioquia
publishDate	2020
url	https://doaj.org/article/cc3d4baca58c4afab5a886d0e09c20d2
work_keys_str_mv	AT elizabethvarelagiraldo effectoflowdensitylipoproteinsandtrehaloseonthequalityofcryopreservedbovinesemen AT alexandrausugasuarez effectoflowdensitylipoproteinsandtrehaloseonthequalityofcryopreservedbovinesemen AT juaneduquecortes effectoflowdensitylipoproteinsandtrehaloseonthequalityofcryopreservedbovinesemen AT jorgegomezoquendo effectoflowdensitylipoproteinsandtrehaloseonthequalityofcryopreservedbovinesemen AT giovannirestrepobetancur effectoflowdensitylipoproteinsandtrehaloseonthequalityofcryopreservedbovinesemen
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Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen

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