Investigation of biofilm formation on a charged intravenous catheter relative to that on a similar but uncharged catheter

Guy A Richards,1,2 Adrian J Brink,3 Ross McIntosh,4 Helen C Steel,5,6 Riana Cockeran5,61Department of Critical Care, Charlotte Maxeke Johannesburg Academic Hospital, 2Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; 3Department of Clinical Microbiology, Ampat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Richards GA, Brink AJ, McIntosh R, Steel HC, Cockeran R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/cc40c2af820344139496b52182f29ae5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cc40c2af820344139496b52182f29ae5
record_format	dspace
spelling	oai:doaj.org-article:cc40c2af820344139496b52182f29ae52021-12-02T01:05:38ZInvestigation of biofilm formation on a charged intravenous catheter relative to that on a similar but uncharged catheter1179-1470https://doaj.org/article/cc40c2af820344139496b52182f29ae52014-06-01T00:00:00Zhttp://www.dovepress.com/investigation-of-biofilm-formation-on-a-charged-intravenous-catheter-r-a17291https://doaj.org/toc/1179-1470 Guy A Richards,1,2 Adrian J Brink,3 Ross McIntosh,4 Helen C Steel,5,6 Riana Cockeran5,61Department of Critical Care, Charlotte Maxeke Johannesburg Academic Hospital, 2Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; 3Department of Clinical Microbiology, Ampath National Laboratory Services, Milpark Hospital, Johannesburg, South Africa; 4Nano-Scale Transport Physics Laboratory, School of Physics, University of the Witwatersrand, Johannesburg, South Africa; 5Medical Research Council Unit for Inflammation and Immunity, Department of Immunology, University of Pretoria, Pretoria, South Africa; 6National Health Laboratory Service, Pretoria, South AfricaAbstract: Catheter-related blood stream infections increase morbidity, mortality, and costs. This study investigated whether Certofix® protect antimicrobial catheters carry a surface charge and whether this inhibits biofilm formation. The capacitance of the catheter surfaces was measured and, to determine if the catheters released ions, distilled water was passed through and current measured as a function of voltage. With probes touching the inner and outer surfaces, capacitance was not voltage-dependent, indicating surfaces were uncharged or carried a similar charge. When one probe penetrated the catheter wall, capacitance was weakly voltage-dependent, indicating the presence of a surface charge. Standard and charged catheters were also exposed to phosphate buffered saline as controls or 2×106 colony forming units/mL (in phosphate buffered saline) of six different microorganisms for 60 or 120 minutes. When the growth of detached bacteria was measured, biofilm formation was significantly reduced, (P<0.05), for charged catheters for all organisms.Keywords: central venous catheters, electrical charge, biofilmRichards GABrink AJMcIntosh RSteel HCCockeran RDove Medical PressarticleMedical technologyR855-855.5ENMedical Devices: Evidence and Research, Vol 2014, Iss default, Pp 219-224 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medical technology R855-855.5
spellingShingle	Medical technology R855-855.5 Richards GA Brink AJ McIntosh R Steel HC Cockeran R Investigation of biofilm formation on a charged intravenous catheter relative to that on a similar but uncharged catheter
description	Guy A Richards,1,2 Adrian J Brink,3 Ross McIntosh,4 Helen C Steel,5,6 Riana Cockeran5,61Department of Critical Care, Charlotte Maxeke Johannesburg Academic Hospital, 2Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; 3Department of Clinical Microbiology, Ampath National Laboratory Services, Milpark Hospital, Johannesburg, South Africa; 4Nano-Scale Transport Physics Laboratory, School of Physics, University of the Witwatersrand, Johannesburg, South Africa; 5Medical Research Council Unit for Inflammation and Immunity, Department of Immunology, University of Pretoria, Pretoria, South Africa; 6National Health Laboratory Service, Pretoria, South AfricaAbstract: Catheter-related blood stream infections increase morbidity, mortality, and costs. This study investigated whether Certofix® protect antimicrobial catheters carry a surface charge and whether this inhibits biofilm formation. The capacitance of the catheter surfaces was measured and, to determine if the catheters released ions, distilled water was passed through and current measured as a function of voltage. With probes touching the inner and outer surfaces, capacitance was not voltage-dependent, indicating surfaces were uncharged or carried a similar charge. When one probe penetrated the catheter wall, capacitance was weakly voltage-dependent, indicating the presence of a surface charge. Standard and charged catheters were also exposed to phosphate buffered saline as controls or 2×106 colony forming units/mL (in phosphate buffered saline) of six different microorganisms for 60 or 120 minutes. When the growth of detached bacteria was measured, biofilm formation was significantly reduced, (P<0.05), for charged catheters for all organisms.Keywords: central venous catheters, electrical charge, biofilm
format	article
author	Richards GA Brink AJ McIntosh R Steel HC Cockeran R
author_facet	Richards GA Brink AJ McIntosh R Steel HC Cockeran R
author_sort	Richards GA
title	Investigation of biofilm formation on a charged intravenous catheter relative to that on a similar but uncharged catheter
title_short	Investigation of biofilm formation on a charged intravenous catheter relative to that on a similar but uncharged catheter
title_full	Investigation of biofilm formation on a charged intravenous catheter relative to that on a similar but uncharged catheter
title_fullStr	Investigation of biofilm formation on a charged intravenous catheter relative to that on a similar but uncharged catheter
title_full_unstemmed	Investigation of biofilm formation on a charged intravenous catheter relative to that on a similar but uncharged catheter
title_sort	investigation of biofilm formation on a charged intravenous catheter relative to that on a similar but uncharged catheter
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/cc40c2af820344139496b52182f29ae5
work_keys_str_mv	AT richardsga investigationofbiofilmformationonachargedintravenouscatheterrelativetothatonasimilarbutunchargedcatheter AT brinkaj investigationofbiofilmformationonachargedintravenouscatheterrelativetothatonasimilarbutunchargedcatheter AT mcintoshr investigationofbiofilmformationonachargedintravenouscatheterrelativetothatonasimilarbutunchargedcatheter AT steelhc investigationofbiofilmformationonachargedintravenouscatheterrelativetothatonasimilarbutunchargedcatheter AT cockeranr investigationofbiofilmformationonachargedintravenouscatheterrelativetothatonasimilarbutunchargedcatheter
_version_	1718403345051287552

Investigation of biofilm formation on a charged intravenous catheter relative to that on a similar but uncharged catheter

Ejemplares similares