Metabolic response of Candida albicans to phenylethyl alcohol under hyphae-inducing conditions.

Phenylethyl alcohol was one of the first quorum sensing molecules (QSMs) identified in C. albicans. This extracellular signalling molecule inhibits the hyphal formation of C. albicans at high cell density. Little is known, however, about the underlying mechanisms by which this QSM regulates the morp...

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Autores principales:	Ting-Li Han, Sergey Tumanov, Richard D Cannon, Silas G Villas-Boas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cc436d9e3eac42c6bb44856176c228b7
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spelling	oai:doaj.org-article:cc436d9e3eac42c6bb44856176c228b72021-11-18T09:00:10ZMetabolic response of Candida albicans to phenylethyl alcohol under hyphae-inducing conditions.1932-620310.1371/journal.pone.0071364https://doaj.org/article/cc436d9e3eac42c6bb44856176c228b72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23951145/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Phenylethyl alcohol was one of the first quorum sensing molecules (QSMs) identified in C. albicans. This extracellular signalling molecule inhibits the hyphal formation of C. albicans at high cell density. Little is known, however, about the underlying mechanisms by which this QSM regulates the morphological switches of C. albicans. Therefore, we have applied metabolomics and isotope labelling experiments to investigate the metabolic changes that occur in C. albicans in response to phenylethyl alcohol under defined hyphae-inducing conditions. Our results showed a global upregulation of central carbon metabolism when hyphal development was suppressed by phenylethyl alcohol. By comparing the metabolic changes in response to phenylethyl alcohol to our previous metabolomic studies, we were able to short-list 7 metabolic pathways from central carbon metabolism that appear to be associated with C. albicans morphogenesis. Furthermore, isotope-labelling data showed that phenylethyl alcohol is indeed taken up and catabolised by yeast cells. Isotope-labelled carbon atoms were found in the majority of amino acids as well as in lactate and glyoxylate. However, isotope-labelled carbon atoms from phenylethyl alcohol accumulated mainly in the pyridine ring of NAD(+)/NADH and NADP(-/)NADPH molecules, showing that these nucleotides were the main products of phenylethyl alcohol catabolism. Interestingly, two metabolic pathways where these nucleotides play an important role, nitrogen metabolism and nicotinate/nicotinamide metabolism, were also short-listed through our previous metabolomics works as metabolic pathways likely to be closely associated with C. albicans morphogenesis.Ting-Li HanSergey TumanovRichard D CannonSilas G Villas-BoasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71364 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ting-Li Han Sergey Tumanov Richard D Cannon Silas G Villas-Boas Metabolic response of Candida albicans to phenylethyl alcohol under hyphae-inducing conditions.
description	Phenylethyl alcohol was one of the first quorum sensing molecules (QSMs) identified in C. albicans. This extracellular signalling molecule inhibits the hyphal formation of C. albicans at high cell density. Little is known, however, about the underlying mechanisms by which this QSM regulates the morphological switches of C. albicans. Therefore, we have applied metabolomics and isotope labelling experiments to investigate the metabolic changes that occur in C. albicans in response to phenylethyl alcohol under defined hyphae-inducing conditions. Our results showed a global upregulation of central carbon metabolism when hyphal development was suppressed by phenylethyl alcohol. By comparing the metabolic changes in response to phenylethyl alcohol to our previous metabolomic studies, we were able to short-list 7 metabolic pathways from central carbon metabolism that appear to be associated with C. albicans morphogenesis. Furthermore, isotope-labelling data showed that phenylethyl alcohol is indeed taken up and catabolised by yeast cells. Isotope-labelled carbon atoms were found in the majority of amino acids as well as in lactate and glyoxylate. However, isotope-labelled carbon atoms from phenylethyl alcohol accumulated mainly in the pyridine ring of NAD(+)/NADH and NADP(-/)NADPH molecules, showing that these nucleotides were the main products of phenylethyl alcohol catabolism. Interestingly, two metabolic pathways where these nucleotides play an important role, nitrogen metabolism and nicotinate/nicotinamide metabolism, were also short-listed through our previous metabolomics works as metabolic pathways likely to be closely associated with C. albicans morphogenesis.
format	article
author	Ting-Li Han Sergey Tumanov Richard D Cannon Silas G Villas-Boas
author_facet	Ting-Li Han Sergey Tumanov Richard D Cannon Silas G Villas-Boas
author_sort	Ting-Li Han
title	Metabolic response of Candida albicans to phenylethyl alcohol under hyphae-inducing conditions.
title_short	Metabolic response of Candida albicans to phenylethyl alcohol under hyphae-inducing conditions.
title_full	Metabolic response of Candida albicans to phenylethyl alcohol under hyphae-inducing conditions.
title_fullStr	Metabolic response of Candida albicans to phenylethyl alcohol under hyphae-inducing conditions.
title_full_unstemmed	Metabolic response of Candida albicans to phenylethyl alcohol under hyphae-inducing conditions.
title_sort	metabolic response of candida albicans to phenylethyl alcohol under hyphae-inducing conditions.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cc436d9e3eac42c6bb44856176c228b7
work_keys_str_mv	AT tinglihan metabolicresponseofcandidaalbicanstophenylethylalcoholunderhyphaeinducingconditions AT sergeytumanov metabolicresponseofcandidaalbicanstophenylethylalcoholunderhyphaeinducingconditions AT richarddcannon metabolicresponseofcandidaalbicanstophenylethylalcoholunderhyphaeinducingconditions AT silasgvillasboas metabolicresponseofcandidaalbicanstophenylethylalcoholunderhyphaeinducingconditions
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Metabolic response of Candida albicans to phenylethyl alcohol under hyphae-inducing conditions.

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