Detection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues

Abstract Recent studies suggest that quorum-sensing molecules may play a role in gut microbiota-host crosstalk. However, whether microbiota produces quorum-sensing molecules and whether those molecules can trans-kingdom transport to the host are still unknown. Here, we develop a UPLC-MS/MS-based ass...

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Autores principales:	Jingchuan Xue, Liang Chi, Pengcheng Tu, Yunjia Lai, Chih-Wei Liu, Hongyu Ru, Kun Lu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/cc476622ca214d0d9c25d5adcce6cf94
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spelling	oai:doaj.org-article:cc476622ca214d0d9c25d5adcce6cf942021-12-02T14:34:03ZDetection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues10.1038/s41522-021-00224-52055-5008https://doaj.org/article/cc476622ca214d0d9c25d5adcce6cf942021-06-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00224-5https://doaj.org/toc/2055-5008Abstract Recent studies suggest that quorum-sensing molecules may play a role in gut microbiota-host crosstalk. However, whether microbiota produces quorum-sensing molecules and whether those molecules can trans-kingdom transport to the host are still unknown. Here, we develop a UPLC-MS/MS-based assay to screen the 27 N-acyl homoserine lactones (AHLs) in the gut microbiota and host. Various AHL molecules are exclusively detected in the cecal contents, sera and livers from conventionally-raised mice but cannot be detected in germ-free mice. Pathogen-produced C4-HSL is detected in the cecal contents and sera of Citrobacter rodentium (C. rodentium)-infected mice, but not found in uninfected controls. Moreover, C. rodentium infection significantly increases the level of multiple AHL molecules in sera. Our findings demonstrate that both commensal and pathogenic bacteria, can produce AHLs that can be detected in host bodies, suggesting that quorum-sensing molecules could be a group of signaling molecules in trans-kingdom microbiota-host crosstalk.Jingchuan XueLiang ChiPengcheng TuYunjia LaiChih-Wei LiuHongyu RuKun LuNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Jingchuan Xue Liang Chi Pengcheng Tu Yunjia Lai Chih-Wei Liu Hongyu Ru Kun Lu Detection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues
description	Abstract Recent studies suggest that quorum-sensing molecules may play a role in gut microbiota-host crosstalk. However, whether microbiota produces quorum-sensing molecules and whether those molecules can trans-kingdom transport to the host are still unknown. Here, we develop a UPLC-MS/MS-based assay to screen the 27 N-acyl homoserine lactones (AHLs) in the gut microbiota and host. Various AHL molecules are exclusively detected in the cecal contents, sera and livers from conventionally-raised mice but cannot be detected in germ-free mice. Pathogen-produced C4-HSL is detected in the cecal contents and sera of Citrobacter rodentium (C. rodentium)-infected mice, but not found in uninfected controls. Moreover, C. rodentium infection significantly increases the level of multiple AHL molecules in sera. Our findings demonstrate that both commensal and pathogenic bacteria, can produce AHLs that can be detected in host bodies, suggesting that quorum-sensing molecules could be a group of signaling molecules in trans-kingdom microbiota-host crosstalk.
format	article
author	Jingchuan Xue Liang Chi Pengcheng Tu Yunjia Lai Chih-Wei Liu Hongyu Ru Kun Lu
author_facet	Jingchuan Xue Liang Chi Pengcheng Tu Yunjia Lai Chih-Wei Liu Hongyu Ru Kun Lu
author_sort	Jingchuan Xue
title	Detection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues
title_short	Detection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues
title_full	Detection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues
title_fullStr	Detection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues
title_full_unstemmed	Detection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues
title_sort	detection of gut microbiota and pathogen produced n-acyl homoserine in host circulation and tissues
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cc476622ca214d0d9c25d5adcce6cf94
work_keys_str_mv	AT jingchuanxue detectionofgutmicrobiotaandpathogenproducednacylhomoserineinhostcirculationandtissues AT liangchi detectionofgutmicrobiotaandpathogenproducednacylhomoserineinhostcirculationandtissues AT pengchengtu detectionofgutmicrobiotaandpathogenproducednacylhomoserineinhostcirculationandtissues AT yunjialai detectionofgutmicrobiotaandpathogenproducednacylhomoserineinhostcirculationandtissues AT chihweiliu detectionofgutmicrobiotaandpathogenproducednacylhomoserineinhostcirculationandtissues AT hongyuru detectionofgutmicrobiotaandpathogenproducednacylhomoserineinhostcirculationandtissues AT kunlu detectionofgutmicrobiotaandpathogenproducednacylhomoserineinhostcirculationandtissues
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Detection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues

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