Astigmatic traction force microscopy (aTFM)

Quantifying rapidly progressing three-dimensional forces generated by cells remains a major challenge in mechanobiology. Here, the authors show that combining traction force microscopy with astigmatic imaging permits sensitive out-of-plane force estimation on the second timescale.

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Autores principales: Di Li, Huw Colin-York, Liliana Barbieri, Yousef Javanmardi, Yuting Guo, Kseniya Korobchevskaya, Emad Moeendarbary, Dong Li, Marco Fritzsche
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/cc4958160dce4580b716a2a465783980
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spelling oai:doaj.org-article:cc4958160dce4580b716a2a4657839802021-12-02T14:25:17ZAstigmatic traction force microscopy (aTFM)10.1038/s41467-021-22376-w2041-1723https://doaj.org/article/cc4958160dce4580b716a2a4657839802021-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22376-whttps://doaj.org/toc/2041-1723Quantifying rapidly progressing three-dimensional forces generated by cells remains a major challenge in mechanobiology. Here, the authors show that combining traction force microscopy with astigmatic imaging permits sensitive out-of-plane force estimation on the second timescale.Di LiHuw Colin-YorkLiliana BarbieriYousef JavanmardiYuting GuoKseniya KorobchevskayaEmad MoeendarbaryDong LiMarco FritzscheNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Di Li
Huw Colin-York
Liliana Barbieri
Yousef Javanmardi
Yuting Guo
Kseniya Korobchevskaya
Emad Moeendarbary
Dong Li
Marco Fritzsche
Astigmatic traction force microscopy (aTFM)
description Quantifying rapidly progressing three-dimensional forces generated by cells remains a major challenge in mechanobiology. Here, the authors show that combining traction force microscopy with astigmatic imaging permits sensitive out-of-plane force estimation on the second timescale.
format article
author Di Li
Huw Colin-York
Liliana Barbieri
Yousef Javanmardi
Yuting Guo
Kseniya Korobchevskaya
Emad Moeendarbary
Dong Li
Marco Fritzsche
author_facet Di Li
Huw Colin-York
Liliana Barbieri
Yousef Javanmardi
Yuting Guo
Kseniya Korobchevskaya
Emad Moeendarbary
Dong Li
Marco Fritzsche
author_sort Di Li
title Astigmatic traction force microscopy (aTFM)
title_short Astigmatic traction force microscopy (aTFM)
title_full Astigmatic traction force microscopy (aTFM)
title_fullStr Astigmatic traction force microscopy (aTFM)
title_full_unstemmed Astigmatic traction force microscopy (aTFM)
title_sort astigmatic traction force microscopy (atfm)
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/cc4958160dce4580b716a2a465783980
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AT lilianabarbieri astigmatictractionforcemicroscopyatfm
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AT yutingguo astigmatictractionforcemicroscopyatfm
AT kseniyakorobchevskaya astigmatictractionforcemicroscopyatfm
AT emadmoeendarbary astigmatictractionforcemicroscopyatfm
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