Two-point magnitude MRI for rapid mapping of brown adipose tissue and its application to the R6/2 mouse model of Huntington disease.

The recent discovery of active brown fat in human adults has led to renewed interest in the role of this key metabolic tissue. This is particularly true for neurodegenerative conditions like Huntington disease (HD), an adult-onset heritable disorder with a prominent energy deficit phenotype. Current...

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Autores principales:	Katrin S Lindenberg, Patrick Weydt, Hans-Peter Müller, Axel Bornstedt, Albert C Ludolph, G Bernhard Landwehrmeyer, Wolfgang Rottbauer, Jan Kassubek, Volker Rasche
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cc4968a994c54fe38c0855de15fcb15f
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spelling	oai:doaj.org-article:cc4968a994c54fe38c0855de15fcb15f2021-11-25T06:03:45ZTwo-point magnitude MRI for rapid mapping of brown adipose tissue and its application to the R6/2 mouse model of Huntington disease.1932-620310.1371/journal.pone.0105556https://doaj.org/article/cc4968a994c54fe38c0855de15fcb15f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25144457/?tool=EBIhttps://doaj.org/toc/1932-6203The recent discovery of active brown fat in human adults has led to renewed interest in the role of this key metabolic tissue. This is particularly true for neurodegenerative conditions like Huntington disease (HD), an adult-onset heritable disorder with a prominent energy deficit phenotype. Current methods for imaging brown adipose tissue (BAT) are in limited use because they are equipment-wise demanding and often prohibitively expensive. This prompted us to explore how a standard MRI set-up can be modified to visualize BAT in situ by taking advantage of its characteristic fat/water content ratio to differentiate it from surrounding white fat. We present a modified MRI protocol for use on an 11.7 T small animal MRI scanner to visualize and quantify BAT in wild-type and disease model laboratory mice. In this application study using the R6/2 transgenic mouse model of HD we demonstrate a significantly reduced BAT volume in HD mice vs. matched controls (n = 5 per group). This finding provides a plausible structural explanation for the previously described temperature phenotype of HD mice and underscores the significance of peripheral tissue pathology for the HD phenotype. On a more general level, the results demonstrate the feasibility of MR-based BAT imaging in rodents and open the path towards transferring this imaging approach to human patients. Future studies are needed to determine if this method can be used to track disease progression in HD and other disease entities associated with BAT abnormalities, including metabolic conditions such as obesity, cachexia, and diabetes.Katrin S LindenbergPatrick WeydtHans-Peter MüllerAxel BornstedtAlbert C LudolphG Bernhard LandwehrmeyerWolfgang RottbauerJan KassubekVolker RaschePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105556 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katrin S Lindenberg Patrick Weydt Hans-Peter Müller Axel Bornstedt Albert C Ludolph G Bernhard Landwehrmeyer Wolfgang Rottbauer Jan Kassubek Volker Rasche Two-point magnitude MRI for rapid mapping of brown adipose tissue and its application to the R6/2 mouse model of Huntington disease.
description	The recent discovery of active brown fat in human adults has led to renewed interest in the role of this key metabolic tissue. This is particularly true for neurodegenerative conditions like Huntington disease (HD), an adult-onset heritable disorder with a prominent energy deficit phenotype. Current methods for imaging brown adipose tissue (BAT) are in limited use because they are equipment-wise demanding and often prohibitively expensive. This prompted us to explore how a standard MRI set-up can be modified to visualize BAT in situ by taking advantage of its characteristic fat/water content ratio to differentiate it from surrounding white fat. We present a modified MRI protocol for use on an 11.7 T small animal MRI scanner to visualize and quantify BAT in wild-type and disease model laboratory mice. In this application study using the R6/2 transgenic mouse model of HD we demonstrate a significantly reduced BAT volume in HD mice vs. matched controls (n = 5 per group). This finding provides a plausible structural explanation for the previously described temperature phenotype of HD mice and underscores the significance of peripheral tissue pathology for the HD phenotype. On a more general level, the results demonstrate the feasibility of MR-based BAT imaging in rodents and open the path towards transferring this imaging approach to human patients. Future studies are needed to determine if this method can be used to track disease progression in HD and other disease entities associated with BAT abnormalities, including metabolic conditions such as obesity, cachexia, and diabetes.
format	article
author	Katrin S Lindenberg Patrick Weydt Hans-Peter Müller Axel Bornstedt Albert C Ludolph G Bernhard Landwehrmeyer Wolfgang Rottbauer Jan Kassubek Volker Rasche
author_facet	Katrin S Lindenberg Patrick Weydt Hans-Peter Müller Axel Bornstedt Albert C Ludolph G Bernhard Landwehrmeyer Wolfgang Rottbauer Jan Kassubek Volker Rasche
author_sort	Katrin S Lindenberg
title	Two-point magnitude MRI for rapid mapping of brown adipose tissue and its application to the R6/2 mouse model of Huntington disease.
title_short	Two-point magnitude MRI for rapid mapping of brown adipose tissue and its application to the R6/2 mouse model of Huntington disease.
title_full	Two-point magnitude MRI for rapid mapping of brown adipose tissue and its application to the R6/2 mouse model of Huntington disease.
title_fullStr	Two-point magnitude MRI for rapid mapping of brown adipose tissue and its application to the R6/2 mouse model of Huntington disease.
title_full_unstemmed	Two-point magnitude MRI for rapid mapping of brown adipose tissue and its application to the R6/2 mouse model of Huntington disease.
title_sort	two-point magnitude mri for rapid mapping of brown adipose tissue and its application to the r6/2 mouse model of huntington disease.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/cc4968a994c54fe38c0855de15fcb15f
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Two-point magnitude MRI for rapid mapping of brown adipose tissue and its application to the R6/2 mouse model of Huntington disease.

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