Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division

Abstract Caenorhabditis briggsae has emerged as a model for comparative biology against model organism C. elegans. Most of its cell fate specifications are completed during embryogenesis whereas its cell growth is achieved mainly in larval stages. The molecular mechanism underlying the drastic devel...

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Autores principales:	Xiaomeng An, Jiaofang Shao, Huoming Zhang, Xiaoliang Ren, Vincy Wing Sze Ho, Runsheng Li, Ming-Kin Wong, Zhongying Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cc4ba9723a49479fa0672c789a64dd3d
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spelling	oai:doaj.org-article:cc4ba9723a49479fa0672c789a64dd3d2021-12-02T15:06:13ZComparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division10.1038/s41598-017-04533-82045-2322https://doaj.org/article/cc4ba9723a49479fa0672c789a64dd3d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04533-8https://doaj.org/toc/2045-2322Abstract Caenorhabditis briggsae has emerged as a model for comparative biology against model organism C. elegans. Most of its cell fate specifications are completed during embryogenesis whereas its cell growth is achieved mainly in larval stages. The molecular mechanism underlying the drastic developmental changes is poorly understood. To gain insights into the molecular changes between the two stages, we compared the proteomes between the two stages using iTRAQ. We identified a total of 2,791 proteins in the C. briggsae embryos and larvae, 247 of which undergo up- or down-regulation between the two stages. The proteins that are upregulated in the larval stages are enriched in the Gene Ontology categories of energy production, protein translation, and cytoskeleton; whereas those upregulated in the embryonic stage are enriched in the categories of chromatin dynamics and posttranslational modification, suggesting a more active chromatin modification in the embryos than in the larva. Perturbation of a subset of chromatin modifiers followed by cell lineage analysis suggests their roles in controlling cell division pace. Taken together, we demonstrate a general molecular switch from chromatin modification to metabolism during the transition from C. briggsae embryonic to its larval stages using iTRAQ approach. The switch might be conserved across metazoans.Xiaomeng AnJiaofang ShaoHuoming ZhangXiaoliang RenVincy Wing Sze HoRunsheng LiMing-Kin WongZhongying ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaomeng An Jiaofang Shao Huoming Zhang Xiaoliang Ren Vincy Wing Sze Ho Runsheng Li Ming-Kin Wong Zhongying Zhao Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division
description	Abstract Caenorhabditis briggsae has emerged as a model for comparative biology against model organism C. elegans. Most of its cell fate specifications are completed during embryogenesis whereas its cell growth is achieved mainly in larval stages. The molecular mechanism underlying the drastic developmental changes is poorly understood. To gain insights into the molecular changes between the two stages, we compared the proteomes between the two stages using iTRAQ. We identified a total of 2,791 proteins in the C. briggsae embryos and larvae, 247 of which undergo up- or down-regulation between the two stages. The proteins that are upregulated in the larval stages are enriched in the Gene Ontology categories of energy production, protein translation, and cytoskeleton; whereas those upregulated in the embryonic stage are enriched in the categories of chromatin dynamics and posttranslational modification, suggesting a more active chromatin modification in the embryos than in the larva. Perturbation of a subset of chromatin modifiers followed by cell lineage analysis suggests their roles in controlling cell division pace. Taken together, we demonstrate a general molecular switch from chromatin modification to metabolism during the transition from C. briggsae embryonic to its larval stages using iTRAQ approach. The switch might be conserved across metazoans.
format	article
author	Xiaomeng An Jiaofang Shao Huoming Zhang Xiaoliang Ren Vincy Wing Sze Ho Runsheng Li Ming-Kin Wong Zhongying Zhao
author_facet	Xiaomeng An Jiaofang Shao Huoming Zhang Xiaoliang Ren Vincy Wing Sze Ho Runsheng Li Ming-Kin Wong Zhongying Zhao
author_sort	Xiaomeng An
title	Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division
title_short	Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division
title_full	Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division
title_fullStr	Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division
title_full_unstemmed	Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division
title_sort	comparative proteome analysis between c . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cc4ba9723a49479fa0672c789a64dd3d
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Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division

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