EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis

Zhilin Wu,1 Chen Chen,1 Bo Zhang,2 Liang Tang,2 Wei Shi,2 Danying Liao,2 Gaohong Di,1 Jacques RJ Davis,1 Hui Wang1 1Anesthesiology Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 2Haematology Depa...

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Autores principales:	Wu Z, Chen C, Zhang B, Tang L, Shi W, Liao D, Di G, Davis JR, Wang H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Tissue factor Atherosclerosis Nanoparticle Target delivery EGFP-EGF1 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cc539813add1468a9d6b1b9bfd8a1e9f
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spelling	oai:doaj.org-article:cc539813add1468a9d6b1b9bfd8a1e9f2021-12-02T04:33:56ZEGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis1178-2013https://doaj.org/article/cc539813add1468a9d6b1b9bfd8a1e9f2019-04-01T00:00:00Zhttps://www.dovepress.com/egfp-egf1-conjugated-polylactic-co-glycolic-acid-nanoparticles-a-new-d-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhilin Wu,1 Chen Chen,1 Bo Zhang,2 Liang Tang,2 Wei Shi,2 Danying Liao,2 Gaohong Di,1 Jacques RJ Davis,1 Hui Wang1 1Anesthesiology Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 2Haematology Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China Background: EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) (PLGA) nanoparticle (ENP) has a specific affinity to tissue factor (TF). The aim of this study was to investigate the target delivery of ENP to plaques and its uptake in a mouse model of atherosclerosis in vivo and in vitro. Materials and methods: Coumarin-6- and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR)-loaded ENPs were synthesized using a double-emulsion method. Mouse vascular smooth muscle cells (VSMCs) were induced with MCP-1 to obtain an increased TF expression. Fluorescence microscopy and flow cytometry assay were performed to examine the uptake of coumarin-6-loaded ENPs in cellular models. An animal model of atherosclerosis was established with an ApoE (-/-) mouse fed with continuous high-fat diets for 14 weeks. DiR-loaded ENPs (DiR-ENPs) were injected via the caudal vein. The distribution of DiR-ENPs was examined through organ imaging and confocal laser scanning microscopy. Results: Results indicated TFs were highly expressed in the cellular model. The uptake of coumarin-6-loaded ENPs was significantly higher than that of common PLGA nanoparticles. Thickening of intima and lipid deposition in the aorta could be observed in atherosclerosis mouse models. Confocal laser scanning microscopy organ imaging showed ENPs accumulated in vessels with atherosclerotic plaques, which coincided with high expressions of TF. Conclusion: This study showed that EGFP-EGF1-conjugated PLGA nanoparticles could be effectively delivered to atherosclerotic plaques in vivo and taken up by VSMCs with high TF expressions in vitro. Thus, it could be a promising carrier for targeted therapy of atherosclerosis. Keywords: tissue factor, atherosclerosis, nanoparticle, target delivery, EGFP-EGF1Wu ZChen CZhang BTang LShi WLiao DDi GDavis JRWang HDove Medical PressarticleTissue factorAtherosclerosisNanoparticleTarget deliveryEGFP-EGF1Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 2609-2618 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tissue factor Atherosclerosis Nanoparticle Target delivery EGFP-EGF1 Medicine (General) R5-920
spellingShingle	Tissue factor Atherosclerosis Nanoparticle Target delivery EGFP-EGF1 Medicine (General) R5-920 Wu Z Chen C Zhang B Tang L Shi W Liao D Di G Davis JR Wang H EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis
description	Zhilin Wu,1 Chen Chen,1 Bo Zhang,2 Liang Tang,2 Wei Shi,2 Danying Liao,2 Gaohong Di,1 Jacques RJ Davis,1 Hui Wang1 1Anesthesiology Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 2Haematology Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China Background: EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) (PLGA) nanoparticle (ENP) has a specific affinity to tissue factor (TF). The aim of this study was to investigate the target delivery of ENP to plaques and its uptake in a mouse model of atherosclerosis in vivo and in vitro. Materials and methods: Coumarin-6- and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR)-loaded ENPs were synthesized using a double-emulsion method. Mouse vascular smooth muscle cells (VSMCs) were induced with MCP-1 to obtain an increased TF expression. Fluorescence microscopy and flow cytometry assay were performed to examine the uptake of coumarin-6-loaded ENPs in cellular models. An animal model of atherosclerosis was established with an ApoE (-/-) mouse fed with continuous high-fat diets for 14 weeks. DiR-loaded ENPs (DiR-ENPs) were injected via the caudal vein. The distribution of DiR-ENPs was examined through organ imaging and confocal laser scanning microscopy. Results: Results indicated TFs were highly expressed in the cellular model. The uptake of coumarin-6-loaded ENPs was significantly higher than that of common PLGA nanoparticles. Thickening of intima and lipid deposition in the aorta could be observed in atherosclerosis mouse models. Confocal laser scanning microscopy organ imaging showed ENPs accumulated in vessels with atherosclerotic plaques, which coincided with high expressions of TF. Conclusion: This study showed that EGFP-EGF1-conjugated PLGA nanoparticles could be effectively delivered to atherosclerotic plaques in vivo and taken up by VSMCs with high TF expressions in vitro. Thus, it could be a promising carrier for targeted therapy of atherosclerosis. Keywords: tissue factor, atherosclerosis, nanoparticle, target delivery, EGFP-EGF1
format	article
author	Wu Z Chen C Zhang B Tang L Shi W Liao D Di G Davis JR Wang H
author_facet	Wu Z Chen C Zhang B Tang L Shi W Liao D Di G Davis JR Wang H
author_sort	Wu Z
title	EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis
title_short	EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis
title_full	EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis
title_fullStr	EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis
title_full_unstemmed	EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis
title_sort	egfp-egf1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/cc539813add1468a9d6b1b9bfd8a1e9f
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EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis

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